Chromosomal banding patterns and karyotype evolution in three pig kidney cell strains (PK15, F and RP)

Echard, G.

doi:10.1007/bf00362307

Cited by 10 publications

(6 citation statements)

References 28 publications

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“…G-banding analysis confirmed that the haploid blastomeres did contain intact haploid genomes (Fig. 1C, D) [27], [28]. These results verified that haploid cells with intact genomes existed in the early-stage PA embryos.…”

Section: Resultssupporting

confidence: 67%

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Karyotype Characterization of In Vivo- and In Vitro-Derived Porcine Parthenogenetic Cell Lines

et al. 2014

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Mammalian haploid cell lines provide useful tools for both genetic studies and transgenic animal production. To derive porcine haploid cells, three sets of experiments were conducted. First, genomes of blastomeres from 8-cell to 16-cell porcine parthenogenetically activated (PA) embryos were examined by chromosome spread analysis. An intact haploid genome was maintained by 48.15% of blastomeres. Based on this result, two major approaches for amplifying the haploid cell population were tested. First, embryonic stem-like (ES-like) cells were cultured from PA blastocyst stage embryos, and second, fetal fibroblasts from implanted day 30 PA fetuses were cultured. A total of six ES-like cell lines were derived from PA blastocysts. No chromosome spread with exactly 19 chromosomes (the normal haploid complement) was found. Four cell lines showed a tendency to develop to polyploidy (more than 38 chromosomes). The karyotypes of the fetal fibroblasts showed different abnormalities. Cells with 19–38 chromosomes were the predominant karyotype (59.48–60.91%). The diploid cells were the second most observed karyotype (16.17%–22.73%). Although a low percentage (3.45–8.33%) of cells with 19 chromosomes were detected in 18.52% of the fetus-derived cell lines, these cells were not authentic haploid cells since they exhibited random losses or gains of some chromosomes. The haploid fibroblasts were not efficiently enriched via flow cytometry sorting. On the contrary, the diploid cells were efficiently enriched. The enriched parthenogenetic diploid cells showed normal karyotypes and expressed paternally imprinted genes at extremely low levels. We concluded that only a limited number of authentic haploid cells could be obtained from porcine cleavage-stage parthenogenetic embryos. Unlike mouse, the karyotype of porcine PA embryo-derived haploid cells is not stable, long-term culture of parthenogenetic embryos, either in vivo or in vitro, resulted in abnormal karyotypes. The porcine PA embryo-derived diploid fibroblasts enriched from sorting might be candidate cells for paternally imprinted gene research.

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Section: Resultssupporting

confidence: 67%

“…5E, F). Normally, the intact haploid porcine genome should contain six telocentric chromosomes [28]. The diploid chromosome spread from pPEF3-2 2N and pPEF3-5 2N cell lines showed a normal diploid karyotype (Table 4) (Fig.…”

Section: Resultsmentioning

confidence: 98%

Karyotype Characterization of In Vivo- and In Vitro-Derived Porcine Parthenogenetic Cell Lines

et al. 2014

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“…PK-15 is a non-neoplastic porcine kidney tubular epithelial cell line [28] , cultured as described earlier [74] .…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial toxicity of triclosan on mammalian cells

et al. 2015

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“…Two clones derived from a library of the porcine cell line PK15 were also characterized (164), but they were not localized chromosomally. It is not clear whether these clones are different from the BAC clones or whether the genomic flanking sequences are different due to massive rearrangements during passaging of the cells over decades, as PK15 cells are malignant and transformed (95). For these six PERV clones, their distribution among individual pigs as well as different breeds, i.e., Large White pigs, miniature pigs, Westran pigs, and wild boars, was analyzed (Table 5).…”

Section: Pervsmentioning

confidence: 99%

Infection Barriers to Successful Xenotransplantation Focusing on Porcine Endogenous Retroviruses

2012

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SUMMARY Xenotransplantation may be a solution to overcome the shortage of organs for the treatment of patients with organ failure, but it may be associated with the transmission of porcine microorganisms and the development of xenozoonoses. Whereas most microorganisms may be eliminated by pathogen-free breeding of the donor animals, porcine endogenous retroviruses (PERVs) cannot be eliminated, since these are integrated into the genomes of all pigs. Human-tropic PERV-A and -B are present in all pigs and are able to infect human cells. Infection of ecotropic PERV-C is limited to pig cells. PERVs may adapt to host cells by varying the number of LTR-binding transcription factor binding sites. Like all retroviruses, they may induce tumors and/or immunodeficiencies. To date, all experimental, preclinical, and clinical xenotransplantations using pig cells, tissues, and organs have not shown transmission of PERV. Highly sensitive and specific methods have been developed to analyze the PERV status of donor pigs and to monitor recipients for PERV infection. Strategies have been developed to prevent PERV transmission, including selection of PERV-C-negative, low-producer pigs, generation of an effective vaccine, selection of effective antiretrovirals, and generation of animals transgenic for a PERV-specific short hairpin RNA inhibiting PERV expression by RNA interference.

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Chromosomal banding patterns and karyotype evolution in three pig kidney cell strains (PK15, F and RP)

Cited by 10 publications

References 28 publications

Karyotype Characterization of In Vivo- and In Vitro-Derived Porcine Parthenogenetic Cell Lines

Karyotype Characterization of In Vivo- and In Vitro-Derived Porcine Parthenogenetic Cell Lines

Mitochondrial toxicity of triclosan on mammalian cells

Infection Barriers to Successful Xenotransplantation Focusing on Porcine Endogenous Retroviruses

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