Chromosomal assignment of porcine microsatellites by use of a somatic cell hybrid mapping panel

Rettenberger, G.; Fredholm, Merete; Fries, R.

doi:10.1111/j.1365-2052.1994.tb00369.x

Animal Genetics

1994

DOI: 10.1111/j.1365-2052.1994.tb00369.x

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Chromosomal assignment of porcine microsatellites by use of a somatic cell hybrid mapping panel

G. Rettenberger

Merete Fredholm²,

R. Fries

Abstract: Summary A well‐established and characterized somatic cell hybrid panel was used to map three polymorphic microsatellites. Microsatellite S0072, representing the linkage group S0007‐S0072, was assigned to porcine chromosome 14. Micro‐satellite S0009, representing the unassigned linkage group EAM‐S0009‐S0071, was assigned tentatively to porcine chromosome 11. Finally, S0062 was tentatively mapped to chromosome 18. S0062 may represent the first marker for porcine chromosome 18.

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Cited by 9 publications

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References 16 publications

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“…Statistical evaluation for concordance C and correlation coefficient cp was performed according to Chevalet and Corpet (1986). The distribution pattern of the amplification product of the OBS gene was compared with the distribution patterns of the porcine reference loci in each somatic cell hybrid (Rettenberger et al 1995a).…”

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Partial characterization of porcine obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids

Neuenschwander¹,

Rettenberger²,

Meijerink³

et al. 1996

Animal Genetics

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Degenerate primers based on human and mouse obesity gene (OBS) sequencing data were used in the reverse transcriptase-polymerase chain reaction (RT-PCR) of total RNA from pig white adipose tissue. Both strands of the resultant pig-specific 325bp DNA fragment were sequenced. Comparison of the obtained sequence with known sequences revealed an 86% identity with the human and 84% identity with the mouse OBS cDNA. The OBS gene was physically mapped to pig chromosome 18 by PCR analysis of somatic cell hybrids, using pig-specific primers. This result is consistent with the recent assignment of the human OBS gene to chromosome 7 and the observation made by comparative mapping that by using a human chromosome 7 specific library two segments of conserved synteny were detected on porcine chromosomes 9 and 18. We conclude the border of conserved synteny to be in the 7q31-7q32 region of the human chromosome.

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mentioning

confidence: 99%

Partial characterization of porcine obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids

Neuenschwander¹,

Rettenberger²,

Meijerink³

et al. 1996

Animal Genetics

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The PiGMaP consortium linkage map of the pig (Sus scrofa)

Haley²,

et al. 1995

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A linkage map of the porcine genome has been developed by segregation analysis of 239 genetic markers. Eighty-one of these markers correspond to known genes. Linkage groups have been assigned to all 18 autosomes plus the X Chromosome (Chr). As 69 of the markers on the linkage map have also been mapped physically (by others), there is significant integration of linkage and physical map data. Six informative markers failed to show linkage to these maps. As in other species, the genetic map of the heterogametic sex (male) was significantly shorter (approximately 16.5 Morgans) than the genetic map of the homogametic sex (female) (approximately 21.5 Morgans). The sex-averaged genetic map of the pig was estimated to be approximately 18 Morgans in length. Mapping information for 61 Type I loci (genes) enhances the contribution of the pig gene map to comparative gene mapping. Because the linkage map incorporates both highly polymorphic Type II loci, predominantly microsatellites, and Type I loci, it will be useful both for large experiments to map quantitative trait loci and for the subsequent isolation of trait genes following a comparative and candidate gene approach.

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Construction of a porcine YAC library and mapping of the cardiac muscle ryanodine receptor gene to Chromosome 14q22?q23

et al. 1995

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Large-scale physical mapping of the porcine genome has been limited because up to now no suitable genomic libraries for this purpose have been available. Therefore, we have constructed a yeast artificial chromosome (YAC) library from porcine lymphocytes. The library was cloned in the amplifiable vector pCGS966. A total of 10080 YAC clones was obtained and has been ordered into 105 96-well microtiter plates. An average insert size of 300 kb was calculated from the analysis of 78 randomly selected clones, giving a one-fold coverage of the porcine genome. To analyze the complexity, we have screened the library for five different genes and isolated four different clones containing parts of three of these genes. One YAC clone harboring parts of the porcine cardiac muscle ryanodine receptor (RYR2) gene allowed us to assign this locus to Chromosome (Chr) 14q22-q23. The data were confirmed by PCR analysis of a rodent-porcine hybrid cell panel.

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Chromosomal assignment of porcine microsatellites by use of a somatic cell hybrid mapping panel

Cited by 9 publications

References 16 publications

Partial characterization of porcine obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids

Partial characterization of porcine obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids

The PiGMaP consortium linkage map of the pig (Sus scrofa)

Construction of a porcine YAC library and mapping of the cardiac muscle ryanodine receptor gene to Chromosome 14q22?q23

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