Chromosomal assignment of a glutamic acid transfer RNA (tRNAGlu) gene to 1p36

Boyd, E.; Theriault, A.; Goddard, John P.; Kalaitsidaki, Marianna; Spathas, D. H.; Connor, J. M.

doi:10.1007/bf00293892

Cited by 21 publications

(15 citation statements)

References 24 publications

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“…Each of these genes is encoded by 15-30 copies on chromosomal band lp36 (Naylor et al, 1984;Boyd et al, 1989;Buckland, 1989) with intergenic distances of up to 125 kb (Van der Drift et al, 1994).…”

Section: Resultsmentioning

confidence: 99%

Balanced translocation in a neuroblastoma patient disrupts a cluster of small nuclear RNA UI and tRNA genes in chromosomal band Ip36

Drift

Chan

Laureys

et al. 1995

Genes Chromosomes & Cancer

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Chromosomal band 1p36 probably harbours several neuroblastoma suppressor genes. A neuroblastoma patient has been described with a constitutional balanced translocation, t(1;17)(p36;q12-21). Cytogenetically, no loss of chromosomal material was visible. The 1p36 translocation breakpoint could therefore have inactivated one allele of a tumour suppressor gene, thus predisposing the patient to develop neuroblastoma. We localized this breakpoint by pulsed field gel electrophoresis, analysis of yeast artificial chromosomes, and fluorescence in situ hybridization. Here we report that the breakpoint is within a large cluster of small nuclear RNA U1 (RNU1) and some tRNA genes (TRE, TRN) on chromosomal band 1p36. The size of this cluster is over two megabases and it contains many other locally repeated sequences. Polyadenylated transcripts were identified for some of these sequences. In addition, the cluster is the target for integration of an adenovirus 5/SV40 hybrid virus. The translocation breakpoint maps distal of this viral integration site and proximal of marker PND.

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Section: Resultsmentioning

confidence: 99%

Balanced translocation in a neuroblastoma patient disrupts a cluster of small nuclear RNA UI and tRNA genes in chromosomal band Ip36

Drift

Chan

Laureys

et al. 1995

Genes Chromosomes & Cancer

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“…At least part of the multicopy genes for RNU1 and the tRNA genes TRE and TRN are functional (Lindgren et al, 1985;Boyd et al, 1989;Buckland, 1989). These genes are found in the 1p as well as in the 1q clusters, indicating that they belong to the core region of an ancestral cluster.…”

Section: Discussionmentioning

confidence: 98%

“…These FISH data show that all MSP and MSPL containing PACs and YACs map to 1p36.2 or 3p21. The weak cross-hybridization of MSP/MSPL PACs to 1q21, 1q42, 1q12, and 1p36.33 most likely results from other sequences in these PACs (like TRE, TRN, and RNU1), which are known to have homologues in these chromosomal regions (Lindgren et al, 1985;Boyd et al, 1989;Buckland, 1989;Van der Drift et al, 1994). Southern blots with DNA of these YACs and PACs were hybridized with probes for TRE and RNU1.…”

Section: Homologues Of the Macrophage Stimulating Protein Gene Are Lomentioning

confidence: 99%

Multiple MSP Pseudogenes in a Local Repeat Cluster on 1p36.2: An Expanding Genomic Graveyard?

et al. 1999

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“…1989). In another report, a human tRNAGlu probe also hybridised to these two sites on human chromosome 1 (Boyd et al, 1989), andMcBride et al (1989) have reported data which suggest that human tRNAGI> genes could also be located at these sites. Therefore, these DNA repeat units, which may be as large as 180 kb in size (Buckland, 1989), could be a major site of the genes for small, untranslated RNA species in the human genome.…”

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confidence: 99%

A primate transfer RNA gene cluster and the evolution of human chromosome 1

Buckland

1992

Cytogenet Genome Res

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The localisation of tRNA^Asn gene clusters in the karyotypes of primates has been studied by means of in situ hybridisation. In the human and orangutan (Pongo pygmaeus) karyotypes there are two such gene clusters, one each on the long and short arms of chromosome 1. Old World monkeys, however, contain both gene clusters on their equivalent of the human chromosome 1 short arm, which can be explained by a pericentric inversion which (amongst other chromosome changes) distinguishes the human and Old World monkey chromosomes 1. The capuchin (Cebus appella), however, a New World monkey, has only one tRNA^Asn gene cluster, at least on the elements equivalent to human chromosome 1. This cluster is located proximal to the centromere on a chromosome that has been tentatively identified (by others) as the equivalent of the long arm of human chromosome 1. Should this prove to be correct, it would indicate that the large primate metacentric came into being in the form found today in the great apes, rather than in the form currently found in Old World monkeys. These data further show that the tRNA^Asn gene cluster has been split in two since before the Old World monkeys and hominids diverged, i.e., over 30 million years ago, and also that the original transfer of these genes from one arm of chromosome 1 to the other was unlikely to have involved a pericentric inversion but, rather, some form of replicative transposition.

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Chromosomal assignment of a glutamic acid transfer RNA (tRNAGlu) gene to 1p36

Cited by 21 publications

References 24 publications

Balanced translocation in a neuroblastoma patient disrupts a cluster of small nuclear RNA UI and tRNA genes in chromosomal band Ip36

Balanced translocation in a neuroblastoma patient disrupts a cluster of small nuclear RNA UI and tRNA genes in chromosomal band Ip36

Multiple MSP Pseudogenes in a Local Repeat Cluster on 1p36.2: An Expanding Genomic Graveyard?

A primate transfer RNA gene cluster and the evolution of human chromosome 1

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