Chromosomal and regional localization of the loci for IGKC, IGGC, ALDB, HOXB, GPT, and PRNP in the American mink (Mustela vison): comparisons with human and mouse

Abstract

Chromosomal localization of the genes for gamma- and kappa-immunoglobulins (IGGC and IGKC, respectively), aldolase B (ALDB), prion protein (PRNP), homeo box B (HOXB), and glutamate pyruvate transaminase (GPT) were determined with the use of mink-rodent hybrid cells. Analysis of segregation of the mink markers and chromosomes in these hybrid cells allowed us to assign the gene for HOXB to Chromosome (Chr) 8, IGGC to Chr 10, PRNP and IGKC to Chr 11, ALDB to Chr 12, and GPT to Chr 14 in mink. Furthermore, using a set of mink-mouse hybrid cells carrying fragments of mink Chr 8 of different sizes, we assigned the gene for HOXB to the pter-p26 region of the short arm of Chr 8. Comparative mapping of the genes of mink, human, and mouse, as well as other mammalian species, demonstrated that the mink genes HOXB, PRNP, ALDB, and IGGC are members of a conserved region shared by many mammalian species in common; the IGKC gene is a member of a conserved region common to carnivores and primates, not rodents; the GPT gene is a member of a syntenic gene group probably unique to the Mustelidae family or carnivores.

70 years of research on the American mink (Neovison vison schreb., 1777) genetics - where are we now?

“…On the basis of structural homology analysis of N. vison chromosomes and dozens of other mammalian species, consisting mainly of G band patterns comparison, fluorescence in situ hybridization, interspecies fluorescence in situ hybridization and comparative mapping of genes, the presence of 32-33 conserved segments has been demonstrated in mink chromosomes (HAMEISTER et al, 1997;CHOWDHARY et al, 1998), and a high degree of synteny and collinearity of gene arrangements on the chromosomes, indicating a high evolutionary conservation of the mammalian carnivore genomes (GRADOV et al, 1983;PERELMAN et al, 2005;FERGUSON-SMITH and TRIFONOV, 2007). KHLEBODAROVA et al (1995) have identified characteristic of American mink syntenic region on chromosome 14, encompassing gene loci for glutamic-pyruvate transaminase (GPT, EC 2.6.1.2), phosphoglycolate phosphatase (PGP, EC 3.1.3.18) and phosphoserine phosphatase (PSP, EC 3.1.3.3 ). It is assumed that this region, which was also found in mammals of other families, evolved in Mustelidae de novo (SEROV, 1998;SEROV and RUBSTOV, 1998).…”