Chromosomal analysis of unfertilized human oocytes

Martin, Renée H.; Mahadevan, Maha M.; Taylor, Patrick J.; Hildebrand, Kathy; Long-Simpson, L.; Peterson, D; Yamamoto, Judy; Fleetham, Judy

doi:10.1530/jrf.0.0780673

Cited by 69 publications

(39 citation statements)

References 22 publications

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“…In our observations, two oocytes with structural anomalies (3.6%) were detected, a percentage similar to that observed by others (Bongso et al, 1988;Martin et al, 1986;Plachot et al, 1986;Wramsby and Fredga, 1987). Fragmentation of chromosomes is the most common structural anomaly, accounting for 20% of all anomalies (Papadopoulos etal., 1989).…”

Section: Discussionsupporting

confidence: 91%

“…It has been suggested that anaphase lag is a source for induction of the hypohaploid oocytes, by loss of chromosomes within the cytoplasm. An excess of hypohaploidy was found in studies of the one-cell embryo in the Chinese hamster (Mikamo and Kamiguchi, 1983), in Syrian hamster oocytes (Martin, 1984) and human oocytes (Wramsby and Fredga, 1987;Martin et al, 1986). One of the oocytes in our study showed 18 polar body and 18 second meiotic chromosomes (Figure 4).…”

Section: Discussionsupporting

confidence: 65%

“…The data available on aneuploidy in human oocytes on the other hand gives an extremely variable range of chromosomal anomaly (Table Ila.b). Wramsby and Fredga (1987), Papadopoulos et al (1989) and Ma et al (1989) detected similar percentages of abnormal numbers of chromosomes, while Martin et al (1986), using a conservative protocol for the evaluation of the frequency of aneuploidy, found only 4% numerical aberrations. In contrast, Veiga et al (1987), applying the same criteria, found values about four times higher.…”

Section: Discussionmentioning

confidence: 86%

“…Under certain conditions, when using various superovulation methods during IVF treatment, anaphase lag occurs more frequently than does non-disjunction. This could be an explanation for the high incidence of hypohaploidy reported by Wramsby and Fredga (1987) and Martin et al (1986) (see Table Ha). At any rate, the incidence of aneuploidy in preimplanation embryos appears to be very high indeed, meaning that a large proportion of such embryos will never develop further (Angell et al, 1983(Angell et al, , 1986Plachot et al, 1988).…”

Section: Discussionmentioning

confidence: 92%

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Abnormal chromosomal arrangements in human oocytes

et al. 1990

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Ninety-one human oocytes, lacking signs of fertilization 50 h after insemination in vitro, were investigated cytogenetically to assess the frequency and type of chromosomal abnormalities. Chromosome spreading permitted adequate karyotyping in 55 oocytes. Non-determined numerical aberrations occurred with the following frequencies: hypohaploidy, 10.9% (6/55), hyperhapJoidy, 14.5% (8/55) and hyperdiploidy, 3.6% (2/55). Total aneuploidy occurred with a frequency of 29.1% and was observed in oocytes from 30 patients. No correlation was found between specific chromosomal aberrations and type of infertility, stimulation treatment or gonadotrophin levels. On the other hand, the frequency of aneuploidy was significantly higher (P < 0.05) in patients > 35 years of age. Two chromosomal complements (3.6%) had structural rearrangements; one oocyte had both structural and numerical chromosomal abnormalities and the other had differently condensed regions on the long arms of three chromosomes from group C. The overall frequency of chromosomal aberrations was 32.7%. Only two samples contained an additional set of polar body chromosomes. Thirteen oocytes presented sperm chromosomes in an arrested stage of premature chromosome condensation of the G, phase and four oocytes showed asynchronous condensation of pronuclear chromosomes. Finally, it was concluded that the high proportion of chromosomal aberrations observed in human oocytes may contribute significantly to abnormal embryonic development in vitro.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 92%

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Abnormal chromosomal arrangements in human oocytes

et al. 1990

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“…45 In noninseminated oocytes, the mean frequency of aneuploidy was 37.3%, ranging from 25 to 57.1%. 46,47 Other authors found that only 4.7% of oocytes had additional material. 37 Recently, testing the 1PBs for chromosomes 13, 16, 18, 21 and 22 in fresh oocytes from women 37 -41 years old, 38.2% were found to be aneuploid.…”

Section: Aneuploidy Ratementioning

confidence: 98%

Analysis of nine chromosome probes in first polar bodies and metaphase II oocytes for the detection of aneuploidies

et al. 2003

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We used fluorescent in situ hybridisation (FISH) to detect nine chromosomes (1,13,15,16,17,18,21,22 and X) in 89 first Polar Bodies (1PBs), from in vitro matured oocytes discarded from IVF cycles. In 54 1PBs, we also analysed the corresponding oocyte in metaphase II (MII) to confirm the results; the other 35 1PBs were analysed alone as when preimplantation genetic diagnosis using 1PB (PGD-1PB) is performed. The frequency of aneuploid oocytes found was 47.5%; if the risk of aneuploidy for 23 chromosomes is estimated, the percentage rises to 57.2%. Missing chromosomes or chromatids found in 1PBs of 1PB/MII doublets were confirmed by MII results in 74.2%, indicating that only 25.8% of them were artefactual. Abnormalities observed in 1PBs were 55.8% whole-chromosome alterations and 44.2% chromatid anomalies. We observed a balanced predivision of chromatids for all chromosomes analysed. Differences between balanced predivision in 1PB and MII were statistically significant (Po0.0001, v 2 test); the 1PB was most affected. The mean abnormal segregation frequency for each chromosome was 0.89% (range 0.52-1.70%); so, each of the 23 chromosomes of an oocyte has a risk of 0.89% to be involved in aneuploidy. No significant differences were observed regarding age, type of abnormality (chromosome or chromatid alterations) or frequency of aneuploidy. Nine of the 35 patients (25.7%) whose 1PB and MII were studied presented abnormalities (extra chromosomes) that probably originated in early oogenesis. Analysis of 1PBs to select euploid oocytes could help patients of advanced age undergoing in vitro fertilization (IVF) treatment.

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Parental age-related aneuploidy in human germ cells and offspring: A story of past and present

Eichenlaub-Ritter

1996

Environ. Mol. Mutagen.

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Chromosomal analysis of unfertilized human oocytes

Cited by 69 publications

References 22 publications

Abnormal chromosomal arrangements in human oocytes

Abnormal chromosomal arrangements in human oocytes

Analysis of nine chromosome probes in first polar bodies and metaphase II oocytes for the detection of aneuploidies

Parental age-related aneuploidy in human germ cells and offspring: A story of past and present

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