Chromosomal Analysis of First-Cleavage Mouse Eggs Fertilized in Caffeine-Containing Medium.

Yoshizawa, Midori; Nakamoto, S.; Fukui, Emiko; Muramatsu, Takashi; Okamoto, Akira

doi:10.1262/jrd.38.107

Cited by 18 publications

(15 citation statements)

References 32 publications

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“…The incidences of hyperdiploidy and hypodiploidy are close to those reported by Yoshizawa et al [22]: 0.7% for hyperdiploidy and 1.3% for hypodiploidy in F1 mouse eggs fertilized in caffeine-free medium. The incidence of aneuploidy is within the range 0 to 3% which has been reported in mouse eggs fertilized in vitro [15,[17][18][19]21].…”

supporting

confidence: 89%

“…The incidences of structural aberrations in F1 and ICR mouse eggs are within the range 0.9 to 2.2% reported in mouse eggs fertilized in vitro [15,20,22] and that (0.4 to 2.2%) in mouse eggs fertilized in vivo [15,27,30,31].…”

mentioning

confidence: 60%

“…The incidence of triploidy in F1 mouse eggs was about the same as that (15.8%) in F1 mouse eggs fertilized in a caffeine-free medium [22]. The triploidy observed in both F1 and ICR eggs is considered to be caused by dispermy because their sexchromosome combinations, XXX, XXY and XYY, appeared in the ratio of 1: 2: 1.…”

mentioning

confidence: 63%

“…The agreement between the observed and the expected values was so complete that the involvement of other cases of triploidy could be excluded. The incidence of polyspermy in fertilization in vitro is known to be affected by some experimental factors, such as the sperm concentration in the fertilization medium [12,13], the dose of PMSG being used for superovulation [17] and the addition of caffeine to the fertilization medium [22]. The present series of experiments were designed to minimize these influences, the difference in the incidence of triploidy observed between F1 eggs and ICR eggs should be ascribed to the difference in the genome.…”

mentioning

confidence: 99%

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Analyses of Developmental Ability of (BALB/c*C57BL/6) F1 and ICR Mouse Embryos Fertilized In Vitro and Their Chromosome at the First Cleavage Division.

Yoshizawa

Takada

Nakamoto

et al. 1993

Journal of Reproduction and Development

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supporting

confidence: 89%

mentioning

confidence: 60%

mentioning

confidence: 63%

mentioning

confidence: 99%

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Analyses of Developmental Ability of (BALB/c*C57BL/6) F1 and ICR Mouse Embryos Fertilized In Vitro and Their Chromosome at the First Cleavage Division.

Yoshizawa

Takada

Nakamoto

et al. 1993

Journal of Reproduction and Development

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“…IVF using RF spermatozoa was conducted according to our previously described methods [17,18]. Electrically fused oocytes and control group oocytes were introduced into insemination media and cultured for 6 h in a 5% CO 2 incubator.…”

Section: In Vitro Fertilization (Ivf)mentioning

confidence: 99%

Cytogenetic Analysis and Developmental Assessment of Mouse Embryos Derived from In Vitro Fertilization of Oocytes Reconstructed by Meiosis-II Chromosome Transplantation

Mitsui

Yoshizawa

2007

Journal of Reproduction and Development

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Abstract. An electrofusion methodology for transferring meiosis-II chromosomes (M-II-t) has not been completely established. The present study compared the use of two temperatures (fusion at 37 C for Group A and 25 C for Group B) during an electrofusion procedure for mouse oocyte M-II-t and investigated the cytogenetic normality and developmental competence of embryos derived from in vitro fertilization using oocytes reconstructed by M-II-t. The M-II-t oocytes were fertilized in vitro and cultured to the blastocyst stage; the resultant embryos were analyzed cytogenetically. Subsequently, chromosomal normality of the resultant embryos at the prometaphase stage of first cleavage division and the integrity of the meiosis-II spindles of the reconstructed oocytes were analyzed. The success rate of electrofusion in Group B was 92.1%, which was significantly different from that in Group A (49.2%) (P<0.05). The fertilization rates (A, 80.7%; B, 77.2%) and development rates (A, 70.9%; B, 65.5%) in the M-II-t groups were significantly lower than those in the control group (95.0 and 92.2%, respectively) (P<0.05). The incidence of chromosomal abnormalities in the Group A embryos (20.5%) at the blastocyst stage was significantly higher than that in the control group embryos (8.5%) (P<0.05), but the incidence of chromosomal abnormalities in Group B (12.5%) was not significantly different compared with the other groups. A temperature of 25 C during the electrofusion procedure for M-IIt resulted in a good fusion rate, good development rate, and efficient production of chromosomally normal blastocysts. Furthermore, the incidence of chromosomal abnormalities in the first cleavage embryos at the prometaphase stage in Group B (9.6%) did not differ significantly from that in the control group (6.6%). The spindle morphology of the M-II-t oocytes in Group B was normal.

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Improvement of embryonic development and production of offspring by transferring meiosis-II chromosomes of senescent mouse oocytes into cytoplasts of young mouse oocytes

Mitsui

Yoshizawa

Matsumoto

et al. 2008

J Assist Reprod Genet

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Chromosomal Analysis of First-Cleavage Mouse Eggs Fertilized in Caffeine-Containing Medium.

Cited by 18 publications

References 32 publications

Analyses of Developmental Ability of (BALB/c*C57BL/6) F1 and ICR Mouse Embryos Fertilized In Vitro and Their Chromosome at the First Cleavage Division.

Analyses of Developmental Ability of (BALB/c*C57BL/6) F1 and ICR Mouse Embryos Fertilized In Vitro and Their Chromosome at the First Cleavage Division.

Cytogenetic Analysis and Developmental Assessment of Mouse Embryos Derived from In Vitro Fertilization of Oocytes Reconstructed by Meiosis-II Chromosome Transplantation

Improvement of embryonic development and production of offspring by transferring meiosis-II chromosomes of senescent mouse oocytes into cytoplasts of young mouse oocytes

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