Chromosomal Aberrations in Normal Human Cells Induced by the Auger Effect via Ca Atoms

The aim was to develop a simple biodosimetry method for as rapid as possible estimation of absorbed radiation doses in victims of radiation accidents, in particular after high-dose exposure. Human peripheral blood lymphocytes (PBL) were gamma-irradiated in vitro with several doses up to 40 Gy stimulated with phytohaemagglutinin-P (PHA-P) for 2 days and their chromosomes condensed prematurely using 50 nm calyculin A. Chromosome lengths of Giemsa-stained G2 prematurely condensed chromosomes (PCC) were measured using image analysing software and the ratio of the longest/shortest chromosome length was calculated. The length ratio (LR) of the longest/shortest Giemsa-stained chromosome s increased with a good correlation to the square root of the radiation dose (D) up to 40 Gy, i.e. LR = (4.90 x D0.5) + 2.14. The LR of the longest/shortest chromosome might be used as an index for estimating the radiation dose. The blood samples should not be cooled until the start of separation/stimulation of the lymphocytes. A rapid and easy estimation of large doses after whole-body exposure was identified by measuring the ratio of the longest/shortest length of Giemsa-stained G2-PCC induced by calyculin A. This simple protocol will be particularly useful for making therapy decisions for victims of ionizing radiation exposure and has potential for use as a biodosimeter for partial-body exposure accidents.

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Chromosomal Aberrations in Normal Human Cells Induced by the Auger Effect via Ca Atoms

Cited by 5 publications

References 7 publications

Comment to the paper “Efficacy of intracerebral delivery of cisplatin in combination with photon irradiation for treatment of brain tumors” from Rousseau et al., in press

Comment to the paper “Efficacy of intracerebral delivery of cisplatin in combination with photon irradiation for treatment of brain tumors” from Rousseau et al., in press

Aspects radiobiologiques des traitements anticancéreux par rayonnement synchrotron : bilan et perspectives

Simple biodosimetry method for cases of high-dose radiation exposure using the ratio of the longest/shortest length of Giemsa-stained drug-induced prematurely condensed chromosomes (PCC)

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