Chromosomal aberrations in a fish, Channa punctata after in vivo exposure to three heavy metals

Yadav, Khushboo; Trivedi, Sunil P.

doi:10.1016/j.mrgentox.2009.05.021

Cited by 42 publications

(15 citation statements)

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“…This observation is consistent with the findings reported by Yadav and Trivedi (2009) in which the exposure of Channa punctatus to mercuric chloride, arsenic trioxide and copper sulphate pentahydrate for a week caused chromatid and chromosome breaks, chromatid and chromosome gaps, along with the ring and di-centric chromosomes in the kidney cells. The findings indicate the genotoxic potential of these metals, even in sub-lethal concentrations (Rakshit and Gangopadhyay, 2015).…”

Section: Discussionsupporting

confidence: 93%

Solvent-less Synthesis, Antimalarial and Toxicity Evaluation of Lumefantrine-Copper Complex in Swiss Mice

Arise¹,

Tella²,

Iyiola³

et al. 2016

Not Sci Biol

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The antimalarial efficacy and safety of mechanically induced solventlessly synthesized lumefantrine-copper complex were investigated in experimental mice. Parasite level in Plasmodium berghei-infected mice treated with lumefantrine -copper complex (LCC) significantly declined (p < 0.05) at day 3 and was comparable with that of chloroquine-treated mice. LCC attained a percentage chemo-suppression which was significantly higher than those of pure lumefantrine and comparable with chloroquine. Pure lumefantrine attained a clearance of 88.52%, chloroquine was 91.95%, while LCC was 95.10%. Administration of lumefantrine, LCC and chloroquine to mice for 7 days caused a significant increase (p < 0.05) in the activities of alkaline phosphatase, acid phosphatase and lactate dehydrogenase in the liver when compared with the control, and a significant reduction (p < 0.05) in the liver and kidney activities of alanine and aspartate aminotransferases when compared with the control. Also, there was a significant decrease (p < 0.05) in the levels of PCV, Hb, RBC and lymphocytes and a significant increase (p < 0.05) in the white blood cells count and neutrophil counts in all the treatment groups when compared with control. Alterations in the biochemical parameters and chromosomal aberration in the organs investigated suggested selective, chromosomal and functional toxicity of the tested drugs.Keywords: chloroquine, chromosomal aberration, lumefantrine-copper complex, malaria, solvent-less synthesis Abbreviations: ALT -alanine aminotransferase, AST -aspartate aminotransferase, b.wt. -body weight, LCC -lumefantrinecopper complex, PCV -packed cell volume, Hb -haemoglobin level, WBC -white blood cells count, RBC -red blood cell count, MCV -mean corpuscular volume, MCH -mean corpuscular, TNMA -total number of metaphases analysed, MCAmetaphases with chromosomal aberration, MI -mitotic index, CA -chromosomal aberration Available online: www.notulaebiologicae.ro

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Section: Discussionsupporting

confidence: 93%

Solvent-less Synthesis, Antimalarial and Toxicity Evaluation of Lumefantrine-Copper Complex in Swiss Mice

Arise¹,

Tella²,

Iyiola³

et al. 2016

Not Sci Biol

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“…They do not only deteriorate the physicochemical equilibrium of the aquatic body, but they also disrupt the food web and bring about morphological, physiological and cytogenetic changes in aquatic inhabitants. Genotoxic studies on aquatic organisms exposed to polluted waters containing heavy metals have implicated DNA damage Matsumoto et al, 2006;Yadav and Trivedi, 2009;Barbosa et al, 2009). The number of fish analysed in each sample is presented between brackets.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Sinos River water genotoxicity using the comet assay in fish

et al. 2010

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The Sinos River, in southern Brazil, is polluted by industrial discharges and untreated urban wastes. Fish genotoxicity biomarkers are valuable parameters for environmental risk assessment. In this study, we used the comet assay to detect genotoxicity due to multiple sources of pollution in the peripheral blood of a native fish species (Hyphessobrycon luetkenii). In addition, we analysed possible DNA damage from aluminum, lead, chromium, copper, nickel, iron and zinc contamination. Water samples were collected seasonally from three sampling sites and the fish were assessed under laboratory conditions. Water chemical analysis showed an increased level of aluminum and iron in most of the samples at sites 2 and 3, located in the middle and lower river course, respectively. The index of DNA damage assessed by the comet assay demonstrated no significant differences in different seasons or at the different sampling sites, while the frequency of cells with DNA damage was higher in water samples collected at sites 1 and 2 during the spring season. None of the metals studied seems to be associated with the increase in the frequency of cells with DNA damage observed during the spring season. The results of this study indicate that the Sinos River is contaminated with substances that are genotoxic to fish, including the waters near the river spring.Keywords: genotoxicity, river pollution, biomarkers, metals. Avaliação da genotoxicidade da água do rio dosSinos utilizando o ensaio cometa em peixes ResumoO rio dos Sinos, no sul do Brasil, é poluído tanto por descargas industriais como por resíduos urbanos não tratados. Os biomarcadores de genotoxicidade em peixes são parâmetros valiosos para a determinação de risco ambiental. Neste estudo, utilizamos o ensaio cometa em sangue periférico de um peixe nativo (Hyphessobrycon luetkenii) para detectar a genotoxicidade devido a múltiplas fontes de poluição. Além disso, analisamos a possível influência da contaminação por alumínio, chumbo, cromo, cobre, níquel, ferro e zinco sobre o dano de DNA. Amostras de água foram coletadas sazonalmente em três pontos de coleta e os peixes foram expostos sob condições de laboratório. A análise química da água mostrou níveis elevados de alumínio e ferro na maioria das amostras dos pontos 2 e 3, localizados nos cursos médio e inferior do rio, respectivamente. O índice de dano de DNA estimado pelo ensaio cometa não demonstrou diferenças significativas tanto entre estações como entre pontos de coleta, enquanto que a frequência de células com dano de DNA apresentou valores superiores nas amostras de água coletadas durante a primavera nos pontos 1 e 2. Nenhum dos metais estudados parece estar associado com o aumento na frequência de células com dano de DNA observado durante a primavera. Os resultados deste estudo indicam que o rio dos Sinos está contaminado com substâncias genotóxicas para os peixes, incluindo as águas próximas à nascente do rio. results. Moreover, genotoxicity assays in fish erythrocytes has not been used to evaluate the genot...

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“…Também foi observada significante redução da toxicidade subcrônica em algas, após a remoção de metais pelo tratamento de efluentes em diferentes localidades dos EUA (Butler et al, 2011). Em conjunto, estes dados corroboram a importante contribuição dos elementos metálicos no efeito tóxico (Magdaleno et al, 2008;Villatoro-Pulido et al, 2008;Asakura et al, 2009;Yadav & Trivedi, 2009). …”

Section: Discussionunclassified

Toxicity Bioassay Validation as a Support for Monitoring Effluent Discharge

Sousa¹,

Padula

Silva

et al. 2013

Ecotoxicol. Environ. Contam.

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Toxicity Bioassay Validation as a Support for Monitoring Effluent Discharge -The present work aimed evaluating the impact of precipitation with sodium hydroxide and sodium sulfide, that clearly reduce the chemical contaminants of the effluent, leading to toxicity reduction. Metal analysis by ICP/AES (Inductively Coupled Plasma/Atomic Emission Spectroscopy), physicochemical analysis (pH) and toxicological analysis (by measuring the production of micronuclei in Allium cepa) were performed in the effluent studied before and after the precipitation. The analysis before treatment showed the extreme acidity of the effluent (pH<1,0), metals above the acceptable level for discharging according to the standards (especially zinc and iron) and significant mutagenicity. After precipitation with sodium sulfide, there was enough reduction in metals concentration to allow the effluent discharging within the limits specified by Conama Resolutions 357/2005 and 430/2011, and with both precipitants the discharging was possible according to the limits specified by 8468/76 Decree (São Paulo State/Brazil) (for the elements studied). There was also significant reduction in mutagenicity after treatment. The use of mutagenicity bioassay with A. cepa as a supplementary protocol to the ones already recommended for effluent analysis is emphasized in the present work.

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Chromosomal aberrations in a fish, Channa punctata after in vivo exposure to three heavy metals

Cited by 42 publications

References 50 publications

Solvent-less Synthesis, Antimalarial and Toxicity Evaluation of Lumefantrine-Copper Complex in Swiss Mice

Solvent-less Synthesis, Antimalarial and Toxicity Evaluation of Lumefantrine-Copper Complex in Swiss Mice

Evaluation of Sinos River water genotoxicity using the comet assay in fish

Toxicity Bioassay Validation as a Support for Monitoring Effluent Discharge

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