Chromosomal aberrations and micronuclei induced in onion (Allium cepa) by gamma-radiation

Effect of Gamma Radiation on Cytogenetic and Growth Endpoints of Allium cepa Seedlings in Long-Term Experiments

“…Previously, it was noted that DNA damage in the cells of living organisms can lead to termination of their growth, as well as to long-term consequences [ 12 ]. However, in our experiments, at a high level of chromosome aberrations, no effect of radiation on root growth was revealed [ 6 ]. The absence of inhibition of growth processes can be explained by both relatively low doses of γ-irradiation and the short duration of our experiments (24 h) [ 6 ].…”

“…However, in our experiments, at a high level of chromosome aberrations, no effect of radiation on root growth was revealed [ 6 ]. The absence of inhibition of growth processes can be explained by both relatively low doses of γ-irradiation and the short duration of our experiments (24 h) [ 6 ]. A number of authors described the inhibition of plant growth parameters after irradiation; however, they used high doses [ 12 – 15 ].…”

“…In toxicological experiments, we used the onion biotest ( Allium -test) [ 6 , 7 ], which has shown itself well in assessing the toxicity of environmental samples [ 8 – 11 ]. For onion seedlings, an increase in the frequency of cytogenetic disorders in cells was found at all doses of γ-irradiation for 24 h, including low doses [ 6 ].…”

“…In toxicological experiments, we used the onion biotest ( Allium -test) [ 6 , 7 ], which has shown itself well in assessing the toxicity of environmental samples [ 8 – 11 ]. For onion seedlings, an increase in the frequency of cytogenetic disorders in cells was found at all doses of γ-irradiation for 24 h, including low doses [ 6 ]. At the maximum absorbed dose of 13 Gy, chromosome aberrations were observed in the majority of onion cells at the anaphase and telophase stages of mitosis, and the number of cells with multiple disorders increased.…”

“…2, plants were irradiated at four dose levels, where the absorbed dose was 1.0, 2.6, 5.2, and 10.7 Gy. The selection of radiation doses was based on the previously obtained dose dependence for cytogenetic disorders of Allium cepa [ 6 ]. The absorbed dose for control plants was 10 –4 Gy.…”