Chromosomal Aberration of Fasciola Hepatica Acetone Powder: In Vitro Study on Human Lymphocytes

Abstract

Fasciola hepatica metazoan trematodes parasite that is living in the liver of their host (sheep, goat and human). It usually cause pathological changes in the parasitized organs. Acetone powder technique is used to prepare partially purified organic material of adult worms. Various concentrations of Fasciola hepatica acetone extract (5 mg/ml, 10 mg /ml , 15mg/ml , 20 mg/ml and 40 mg /ml) are used on human lymphocyte culture for studying some chromosomal aberration. Chromosomal aberrations includes Isogap, Breaks and Dicentrics for chromosomes; Gaps and deletion for chromatids; and mitotic index (MI). This In-vitro study revealed that acetone powder extract of Fasciola hepatica parasites causing various genotoxicity effects in the human lymphocyte cells. These effects are ascending in their increasing in significance differences (P< 0.001) for all treatment against control as concentration of acetone extract is increased.