Chromosomal aberration arises during somatic reprogramming to pluripotent stem cells

Liu, Xinyu; Li, Conghui; Zheng, Kang; Zhao, Xiaofeng; Xu, Xiaofeng; Yang, Anli; Yi, Min; Tao, Huaping; Xie, Binghua; Qiu, Mengsheng; Yang, Junlin

doi:10.1186/s13008-020-00068-z

Cited by 17 publications

(12 citation statements)

References 47 publications

(59 reference statements)

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“…Western blotting was carried out as previously described (Liu et al 2020 ). Primary antibodies were used as follows: anti-Olig2 (1:3000), anti-GFAP (1:1000), anti-Nestin (1:1000, MA1-110, Invitrogen), anti-Erk1/2 (1:5000, ab184699, Abcam), and anti-p-Erk1/2 (1:5000, ab76299, Abcam).…”

Section: Methodsmentioning

confidence: 99%

EGF signaling promotes the lineage conversion of astrocytes into oligodendrocytes

Liu

et al. 2022

Mol Med

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Background The conversion of astrocytes activated by nerve injuries to oligodendrocytes is not only beneficial to axonal remyelination, but also helpful for reversal of glial scar. Recent studies have shown that pathological niche promoted the Sox10-mediated astrocytic transdifferentiation to oligodendrocytes. The extracellular factors underlying the cell fate switching are not known. Methods Astrocytes were obtained from mouse spinal cord dissociation culture and purified by differential adherent properties. The lineage conversion of astrocytes into oligodendrocyte lineage cells was carried out by Sox10-expressing virus infection both in vitro and in vivo, meanwhile, epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) inhibitor Gefitinib were adopted to investigate the function of EGF signaling in this fate transition process. Pharmacological inhibition analyses were performed to examine the pathway connecting the EGF with the expression of oligodendrogenic genes and cell fate transdifferentiation. Results EGF treatment facilitated the Sox10-induced transformation of astrocytes to O4+ induced oligodendrocyte precursor cells (iOPCs) in vitro. The transdifferentiation of astrocytes to iOPCs went through two distinct but interconnected processes: (1) dedifferentiation of astrocytes to astrocyte precursor cells (APCs); (2) transformation of APCs to iOPCs, EGF signaling was involved in both processes. And EGF triggered astrocytes to express oligodendrogenic genes Olig1 and Olig2 by activating extracellular signal-regulated kinase 1 and 2 (Erk1/2) pathway. In addition, we discovered that EGF can enhance astrocyte transdifferentiation in injured spinal cord tissues. Conclusions These findings provide strong evidence that EGF facilitates the transdifferentiation of astrocytes to oligodendrocytes, and suggest that targeting the EGF-EGFR-Erk1/2 signaling axis may represent a novel therapeutic strategy for myelin repair in injured central nervous system (CNS) tissues.

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Section: Methodsmentioning

confidence: 99%

EGF signaling promotes the lineage conversion of astrocytes into oligodendrocytes

Liu

et al. 2022

Mol Med

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“…Chromosomal mutations were detected in iPSCs following long-term culture. Therefore, karyotype analysis is a standard procedure and has become a crucial step to determine the normal numbers of chromosomes of the iPS cell line (Liu et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Molecular signature and colony morphology affect in vitro pluripotency of porcine induced pluripotent stem cells

Setthawong

Phakdeedindan

Techakumphu

et al. 2021

Reprod Domestic Animals

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Pig is a potential animal model for biomedical research as the organ size, physiology and immunology of pigs are resemblance to humans (Ye et al., 2014). Moreover, long lifespan of pigs has advantage for long-term study of human diseases following cell transplantation and experimental surgery (Ezashi et al., 2016;Shiue et al., 2016).The genetic modifications via cell reprogramming technology have also held a great promise to improve the efficiency of regenerative medicine and pig production (Gutierrez et al., 2015). However,

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“…The process of generating a hPSC line is lengthy and can be stressful for the cell, potentially allowing for the accumulation of deleterious genomic and phenotypic changes along the way [ 84 ]. Therefore, it is important that quality checks are performed on each cell line to ensure any phenotypes are not merely a consequence of the generation process.…”

Section: Generation Of Human Pluripotent Stem Cell Mitochondrial Disease Modelsmentioning

confidence: 99%

Modelling Mitochondrial Disease in Human Pluripotent Stem Cells: What Have We Learned?

McKnight

Low

Elliott

et al. 2021

IJMS

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Mitochondrial diseases disrupt cellular energy production and are among the most complex group of inherited genetic disorders. Affecting approximately 1 in 5000 live births, they are both clinically and genetically heterogeneous, and can be highly tissue specific, but most often affect cell types with high energy demands in the brain, heart, and kidneys. There are currently no clinically validated treatment options available, despite several agents showing therapeutic promise. However, modelling these disorders is challenging as many non-human models of mitochondrial disease do not completely recapitulate human phenotypes for known disease genes. Additionally, access to disease-relevant cell or tissue types from patients is often limited. To overcome these difficulties, many groups have turned to human pluripotent stem cells (hPSCs) to model mitochondrial disease for both nuclear-DNA (nDNA) and mitochondrial-DNA (mtDNA) contexts. Leveraging the capacity of hPSCs to differentiate into clinically relevant cell types, these models permit both detailed investigation of cellular pathomechanisms and validation of promising treatment options. Here we catalogue hPSC models of mitochondrial disease that have been generated to date, summarise approaches and key outcomes of phenotypic profiling using these models, and discuss key criteria to guide future investigations using hPSC models of mitochondrial disease.

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Chromosomal aberration arises during somatic reprogramming to pluripotent stem cells

Cited by 17 publications

References 47 publications

EGF signaling promotes the lineage conversion of astrocytes into oligodendrocytes

EGF signaling promotes the lineage conversion of astrocytes into oligodendrocytes

Molecular signature and colony morphology affect in vitro pluripotency of porcine induced pluripotent stem cells

Modelling Mitochondrial Disease in Human Pluripotent Stem Cells: What Have We Learned?

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