Chromomycin A 3 Stains Nucleolus Organizer Regions of Fish Chromosomes

Amemiya, Chris T.; Gold, John R.

doi:10.2307/1444915

Cited by 132 publications

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“…We found that the Ag-NOR sites were positively stained by the CMA 3 fluorochrome (Figure 2b), suggesting that the rDNA loci of both Characidium species studied in may contain spacer sequences or NOR-associated heterochromatin rich in GC base pairs. On the other hand, these data suggest that the C-band positive segments found in both species are not rich in GC base pairs, a characteristic which has been commonly found in several fish species (Amemiya and Gold, 1986;Maistro et al, 2002;Fonseca et al, 2003;among others).…”

Section: Discussionsupporting

confidence: 55%

Cytogenetic divergence between two sympatric species of Characidium (Teleostei, Characiformes, Crenuchidae) from the Machado River, Minas Gerais, Brazil

Silva

Maistro

2006

Genet. Mol. Biol.

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Cytogenetic studies were performed on two sympatric species of Characidium, C. gomesi and C. cf. zebra, from the Grande River basin, Minas Gerais State, Brazil. Although both species had a chromosome number of 50 with a karyotype exclusively consisting of meta-and submetacentric chromosomes, interspecific diversity was detected concerning the size of the two first chromosome pairs of the karyotypes. Active nucleolus organizer regions (NORs) were located at the terminal position on the long arm of the 17 th pair of C. gomesi and at subterminal position on the long arm of the 23 rd pair of C. cf. zebra. For both species the fluorochrome CMA 3 stained only the NOR-bearing pair of chromosomes. The heterochromatin pattern also showed some differentiation between these species restricted to the centromeric or pericentromeric region of C. cf. zebra and practically absent in C. gomesi. These data are discussed concerning chromosome diversification in this fish group.

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Section: Discussionsupporting

confidence: 55%

Cytogenetic divergence between two sympatric species of Characidium (Teleostei, Characiformes, Crenuchidae) from the Machado River, Minas Gerais, Brazil

Silva

Maistro

2006

Genet. Mol. Biol.

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“…Such an association between GC-rich heterochromatin and ribosomal genes seems to be a feature of NORs quite common in teleosts, as originally proposed by Amemiya & Gold (1986), although the nature of the association has to be clarified, i.e.…”

Section: Resultsmentioning

confidence: 71%

“…Such heteromorphism supports the current opinion that the NOR-associated heterochromatin might be the cause of higher rates of rearrangements of these chromosome regions. On the other hand, all populations showed the same pattern of number of NORs and locations shared also by specimens from Texas (Amemiya & Gold, 1986), and such homogeneity in specimens from locales so far apart is undoubtedly worth noting, considering that variability of NORs can be very conspicuous in some species of fish, as in salmonids (Phillips & Hartley, 1988;Phillips et a!., 1989;Pendas et a!., 1993a) and in the genus Poecilia (Sola eta!., 1992a,b;1993).…”

Section: Resultsmentioning

confidence: 95%

“…Although the karyotype of M cephalus is already known (Table 1), there are no data from differential staining techniques, except for observations on nucleolar organizer regions (NOR5) by Amemiya & Gold (1986). Moreover, previous studies cover only few localities from the wide species range and specimens from more than one collecting site have never been observed in the same laboratory.…”

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confidence: 99%

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Cytogenetic analysis of global populations of Mugil cephalus (striped mullet) by different staining techniques and fluorescent in situ hybridization

Rossi¹,

Crosetti

Gornung

et al. 1996

Heredity

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The present paper reports the results of cytogenetic analysis carried out on several scattered populations of the striped mullet, Mugil cephalus, the most widespead among mugilid species. The karyotype was investigated through Ag-staining, C-banding, fluorochrome-staining (chromomycin A3/DAPI) and fluorescent in situ hybridization with rDNA genes. All populations showed the same chromosome number and morphology and no changes were detected in heterochromatin and NORs. Therefore, neither population-nor sex-specific marker chromosomes were identified. In some of the specimens, NOR size heteromorphism was detected. Results are discussed with respect to karyotype and ribosomal cistrons organization and to cytotaxonomic implications.

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“…Relationships between NORs and GC-specific fluorochromes have been reported in several groups of fish (Amemiya and Gold, 1986). In most cases, CMA 3 staining produces markings that are only coincident with the NORs observed in Pimelodella sp, P. taenioptera, and I. schubarti.…”

Section: Discussionmentioning

confidence: 87%

Cytogenetic characterization of species of the family Heptapteridae (Teleostei: Siluriformes) from the Cuiabá and Ivaí River Basins, Brazil

Barbosa¹,

Ferreira²,

Prizon³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. We cytogenetically characterized three species of Heptapteridae (Pimelodella sp, Pimelodella taenioptera, and Imparfinis schubarti) by investigating the distribution of constitutive heterochromatin and nucleolar organizer regions by silver nitrate impregnation (Ag-NOR) and fluorescence in situ hybridization. Pimelodella sp showed had a diploid number (2n) = 46 chromosomes, 26m + 10sm + 10st, and FN = 92; P. taenioptera, 2n = 52 chromosomes, 26m + 22sm + 4st, and FN = 104; and I. schubarti, 2n = 58 chromosomes, 28m + 28sm + 2st, and FN = 116. The two Pimelodella species had Ag-NORs sites on the submetacentric pair 14, located on the short arm in terminal position. In I. schubarti, the Ag-NORs sites were in an interstitial position on the long arm of the metacentric pair 1. C-banding revealed that Pimelodella sp contained a small amount of constitutive heterochroma- Cytogenetics of species of the family Heptapteridae tin, whereas P. taenioptera contained a higher number of heterochromatic regions, in the pericentromeric, interstitial, and telomeric positions. I. schubarti had markers in centromeric and telomeric regions of a few chromosomes, and a large pericentromeric block on pair 1. Fluorochrome chromomycin A 3 (CMA 3 ) staining revealed positive signals on pair 14 in both Pimelodella species. Treatment with 4ꞌ,6-diamidino-2-phenylindole (DAPI) revealed no markings in P. taenioptera, but an interstitial marking on the long arm of pair 14 in Pimelodella sp. In I. schubarti, positive signals of CMA 3 were detected in the first pair, but negative signals were detected for DAPI staining. These results contribute to the karyotypic description of the less-studied species in the Brazilian Midwest.

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Chromomycin A 3 Stains Nucleolus Organizer Regions of Fish Chromosomes

Cited by 132 publications

References 0 publications

Cytogenetic divergence between two sympatric species of Characidium (Teleostei, Characiformes, Crenuchidae) from the Machado River, Minas Gerais, Brazil

Cytogenetic divergence between two sympatric species of Characidium (Teleostei, Characiformes, Crenuchidae) from the Machado River, Minas Gerais, Brazil

Cytogenetic analysis of global populations of Mugil cephalus (striped mullet) by different staining techniques and fluorescent in situ hybridization

Cytogenetic characterization of species of the family Heptapteridae (Teleostei: Siluriformes) from the Cuiabá and Ivaí River Basins, Brazil

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