Chromogranin-A Regulates Macrophage Function and the Apoptotic Pathway in Murine DSS colitis

Eissa, Nour; Hussein, Hayam; Kermarrec, Laëtitia; Ali, Ahmed; Marshall, Aaron J.; Metz‐Boutigue, Marie‐Hélène; Hendy, Geoffrey N.; Bernstein, Çharles N.; Ghia, Jean–Eric

doi:10.1007/s00109-017-1613-6

Cited by 33 publications

(25 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several reports also linked the beneficial effects of probiotics with the upregulation of immune related genes, which refer to appropriate functions of the innate immune response 8 , 9 , 21 , 22 . However, an aberrant immune activation results in detrimental effects that impact overall health 11 , 23 – 26 supporting the potential immunomodulatory of mixed bacillus species by accelerating the recovery from the acute phase innate immune response in this study.…”

Section: Discussionsupporting

confidence: 73%

Mixed Bacillus Species Enhance the Innate Immune Response and Stress Tolerance in Yellow Perch Subjected to Hypoxia and Air-Exposure Stress

Eissa

Wang

Yao

et al. 2018

Sci Rep

Self Cite

View full text Add to dashboard Cite

Stress enhances the disease susceptibility in fish by altering the innate immune responses, which are essential defense mechanisms. The use of probiotics is increasingly popular in the aquaculture industry. Yellow perch is a promising candidate for aquaculture. We investigated the efficiency of a mixed Bacillus species in minimizing the potential problems resulting from husbandry practices such as hypoxia and exposure to air in yellow perch. We showed that hypoxia and air exposure conditions induced a significant reduction in the early innate immune response (lysozyme activity, interferon-induced-GTP-binding protein-Mx1 [mx], interleukin-1β [il1β], serum amyloid-A [saa]), and a substantial increase in cortisol, heat shock protein (Hsp70), glutathione peroxidase (Gpx), superoxide dismutase (Sod1) that associated with a decline in insulin-like growth factor-1 (Igf1). Mixed Bacillus species administration improved the early innate responses, reduced cortisol, Hsp70, Gpx and Sod1, and elevated Igf1 levels. Bacillus species treated group showed faster recovery to reach the baseline levels during 24 h compared to untreated group. Therefore, mixed Bacillus species may enhance yellow perch welfare by improving the stress tolerance and early innate immune response to counterbalance the various husbandry stressors. Further studies are warranted to investigate the correlations between the aquaculture practices and disease resistance in yellow perch.

show abstract

Section: Discussionsupporting

confidence: 73%

Mixed Bacillus Species Enhance the Innate Immune Response and Stress Tolerance in Yellow Perch Subjected to Hypoxia and Air-Exposure Stress

Eissa

Wang

Yao

et al. 2018

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The experiments were performed as previously described [6,14,22]. Briefly, colonic tissue was lysed in RIPA buffer (50 mmol/L Tris pH 8, 0.1% SDS, 0.5% deoxycholate, 1% NP-40, 150 mmol/L NaCl, 1 tablet complete mini protease inhibitor/10 mL, (Roche Diagnostics GmbH, Mannheim, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Intestinal epithelial and neuroendocrine cells throughout the GI tract express a large number of peptides that have complementary activities and play fundamental roles in regulating the host-microbe interactions and mucosal immune responses that underlie the pathogenesis of IBD [11,12,13]. In the gastrointestinal tract, enterochromaffin cells secrete the neuroendocrine pro-hormone CHGA [11], which is elevated in patients with IBD and plays a crucial role in the development of IBD [11,14]. Processing of CHGA gives rise to several peptides that play a significant role in intestinal mucosal immunity [6,11,15,16].…”

Section: Introductionmentioning

confidence: 99%

Catestatin Regulates Epithelial Cell Dynamics to Improve Intestinal Inflammation

et al. 2018

Self Cite

View full text Add to dashboard Cite

Ulcerative colitis (UC) is characterized by aberrant regulation of tight junctions (TJ), signal transducer and activator of transcription 3 (STAT3), and interleukin (IL)-8/18, which lead to intestinal barrier defects. Catestatin (CST), an enterochromaffin-derived peptide, regulates immune communication and STAT-3 in the inflamed intestine. Here, we investigated the effects of CST during the development of inflammation using human biopsies from patients with active UC, human colonic epithelial cells (Caco2), and an experimental model of UC (dextran sulfate sodium [DSS]-colitis). In UC patients, the protein and mRNA level of CST was significantly decreased. Colonic expression of CST showed a strong positive linear relationship with TJ proteins and STAT3, and a strong negative correlation with IL-8 and IL-18. Intra-rectal administration of CST reduced the severity of experimental colitis, IL-18 colonic levels, maintained TJ proteins and enhanced the phosphorylation of STAT3. CST administration increased proliferation, viability, migration, TJ proteins, and p-STAT3 levels, and reduced IL-8 & IL-18 in LPS- & DSS-induced Caco2 cell epithelial injury, and the presence of STAT-3 inhibitor abolished the beneficial effect of CST. In inflammatory conditions, we conclude that CST could regulate intestinal mucosal dynamic via a potential STAT3-dependent pathway that needs to be further defined. Targeting CST in intestinal epithelial cells (IECs) should be a promising therapeutic approach such as when intestinal epithelial cell homeostasis is compromised in UC patients.

show abstract

“…Relative gene expression was calculated using the differences in the threshold cycle (ΔCt) number between the reference gene and the optimal target gene (Eissa et al, ; Eissa, Kermarrec, et al, ). Differences in the threshold cycle (ΔCt) number between the target genes and mouse eukaryotic elongation factor 2 and human TATA‐box binding protein (optimal reference genes) were used to normalize expression (Eissa et al, ). Human and mouse primer sequences for the target gene markers are provided in Tables and .…”

Section: Methodsmentioning

confidence: 99%

Semaphorin‐3E attenuates intestinal inflammation through the regulation of the communication between splenic CD11C⁺ and CD4⁺CD25⁻ T‐cells

Kermarrec

Eissa

Wang

et al. 2019

British J Pharmacology

Self Cite

View full text Add to dashboard Cite

Background and Purpose: An alteration in the communication between the innate and adaptive immune cells is a hallmark of ulcerative colitis (UC). Semaphorin-3E (SEMA3E), a secreted guidance protein, regulates various immune responses. Experimental Approach: We investigated the expression of SEMA3E in colonic biopsies of active UC patients and its mechanisms in Sema3e −/− mice using an experimental model of UC.Key Results: SEMA3E level was decreased in active UC patients and negatively correlated with pro-inflammatory mediators. Colonic expression of SEMA3E was reduced in colitic Sema3e +/+ mice, and recombinant (rec-) Plexin-D1 treatment exacerbated disease severity. In vivo rec-SEMA3E treatment restored SEMA3E level in colitic Sema3e +/+ mice. In Sema3e −/− mice, disease severity was increased, and rec-SEMA3E ameliorated these effects. Lack of Sema3e increased the expression of CD11c and CD86 markers. Colitic Sema3e −/− splenocytes and splenic CD11c + cells produced more IL-12/23 and IFN-γ compared to Sema3e +/+ , and rec-SEMA3E reduced their release as much as NF-κB inhibitors, whereas an NF-κB activator increased their production and attenuated the effect of rec-SEMA3E. Colitic Sema3e −/− splenic CD11c + /CD4 + CD25 − T-cell co-cultures produced higher concentrations of IFN-γ and IL-17 when compared to colitic Sema3e +/+ splenic cell co-cultures, and rec-SEMA3E decreased these effects. In vitro, anti-IL-12p19 and -12p35 antibodies and rec-IL-12 and -23 treatment confirmed the crosstalk between CD11c + and CD4 + CD25 − T-cells.Conclusion and Implications: SEMA3E is reduced in colitis and modulates colonic inflammation by regulating the interaction between CD11c + and CD4 + CD25 − T-cells via an NF-κB-dependent mechanism. Thus, SEMA3E could be a potential therapeutic target for UC patients.

show abstract

Chromogranin-A Regulates Macrophage Function and the Apoptotic Pathway in Murine DSS colitis

Cited by 33 publications

References 47 publications

Mixed Bacillus Species Enhance the Innate Immune Response and Stress Tolerance in Yellow Perch Subjected to Hypoxia and Air-Exposure Stress

Mixed Bacillus Species Enhance the Innate Immune Response and Stress Tolerance in Yellow Perch Subjected to Hypoxia and Air-Exposure Stress

Catestatin Regulates Epithelial Cell Dynamics to Improve Intestinal Inflammation

Semaphorin‐3E attenuates intestinal inflammation through the regulation of the communication between splenic CD11C⁺ and CD4⁺CD25⁻ T‐cells

Contact Info

Product

Resources

About

Chromogranin-A Regulates Macrophage Function and the Apoptotic Pathway in Murine DSS colitis

Cited by 33 publications

References 47 publications

Mixed Bacillus Species Enhance the Innate Immune Response and Stress Tolerance in Yellow Perch Subjected to Hypoxia and Air-Exposure Stress

Mixed Bacillus Species Enhance the Innate Immune Response and Stress Tolerance in Yellow Perch Subjected to Hypoxia and Air-Exposure Stress

Catestatin Regulates Epithelial Cell Dynamics to Improve Intestinal Inflammation

Semaphorin‐3E attenuates intestinal inflammation through the regulation of the communication between splenic CD11C+ and CD4+CD25− T‐cells

Contact Info

Product

Resources

About

Semaphorin‐3E attenuates intestinal inflammation through the regulation of the communication between splenic CD11C⁺ and CD4⁺CD25⁻ T‐cells