Chromogenic media for MRSA diagnostics

Abstract

For the past decade, a number of chromogenic media for methicillin-resistance Staphylococcus aureus (MRSA) detection have been developed and applied, including Oxoid Brilliance™ MRSA, CHROMagar™ MRSA, BBL™ CHROMagar™ MRSA, MRSASelect and chromID MRSA. The advantages of these chromogenic media offers direct detection of visible staphylococcal colonies, coupled with the use of chromogenic enzymatic substrates that can be hydrolyzed by S. aureus to confirm species or strain identification. BBL™ CHROMagar™ MRSA and MRSASelect are designed for detection of nasal colonization by MRSA, while CHROMagar™ MRSA, Oxoid Brilliance™ MRSA and chromID MRSA are readily applied in bacterial screening. This review summarizes the characteristics, principles and capacities of these selective media, and focuses on comparison of different chromogenic media.

Çeşitli Klinik Örneklerden Izole Edilen Staphylococcus aureus Suşlarında Metisilin Direncinin Araştırılmasında Konvansiyonel PCR Yöntemi ile Sefoksitin Disk Difüzyon, Otomatize Sistem ve Chromogenic MRSA Agar Yöntemlerinin Karşılaştırılması

“…Chromogenic media have been preferred because they are faster than conventional methods and appropriate to use statistically according to many articles in the literature (Xu et al, 2016, Malhotra-Kumar et al, 2008, Sürücüoğlu et al, 2011, Denys et al, 2013. There are many studies that have investigated whether various chromogenic media are comparable and suitable for use (Strulens et al, Uzun et al, 2013, Cesur et al, 2014Von Eiff et al, 2008, Denys et al, 2013 .…”

“…Most studies revealed that the use of chromogenic media for MRSA identification can generally, provide acceptable diagnostic performance, although specificity and sensitivity of chromogenic media varies amongst suppliers. The performance of chromogenic media is influenced by several variables, including specimen type, incubation time, broth enrichment step, or investigator perception (Xu Z, et al, 2016).…”

“…The aim of using these media is to diagnose MRSA infection in one step and to start the treatment as soon as possible. The first developed chromogenic medium for MRSA identification is Chromagar ™ MRSA (Chromagar microbiology, Paris, France) (Xu et al, 2016). Samples were inoculated onto sheep-blood agar and incubated at 37°C for 24 hours.…”

On the challenges of detecting wholeStaphylococcus aureuscells with biosensors

“…Most methods used so far for the detection of bacterial pathogens rely on microbial culture on selective or chromogenic media (Xu et al . ), ELISA immunoassays (Yazdankhah et al . ), selective gene amplification and detection (Tombelli et al .…”

An epidemic CC1-MRSA-IV clone yields false negative test results in molecular MRSA identification assays: a note of caution

“…Effective IPC implementation requires the rapid detection of MRSA. Traditionally, this has been achieved using selective growth media containing methicillin or cefoxitin, salt and a chromogenic substrate (for an overview, see (8)). The latter facilitates the quick differentiation of MRSA from other, clinically less relevant staphylococcal species that may also harbour mec genes.…”