Chromogenic Agar Assisted Screening of Pathogenic Bacteria from Butcher shops Wastewater Effluent and their Antibiotic Susceptibility Profiling

Abstract

The research utilized sophisticated technique called chromogenic agar and the Vitek 2 system to examine the bacterial content of fourteen samples of butcher’s shops effluent water, revealing thirty different bacterial species with varying levels of antibiotic resistance, including Staphylococcus aureus, Enterococcus faecalis, Citrobacter freundii, Escherichia coli, and Klebsiella pneumoniae,. The Vitek 2 system verified the initial identification of bacteria with 100% accuracy, providing additional evidence for the reliability of the chromogenic agar method. The Gram staining results revealed the presence of both gram-positive and gram-negative bacteria. These findings emphasize the importance of proper sanitation and hygiene practices to minimize the potential health risks associated with bacterial contamination in effluent water from butcher’s shops.