Chromobacterium vaccinii sp. nov., isolated from native and cultivated cranberry (Vaccinium macrocarpon Ait.) bogs and irrigation ponds

Abstract

A large number of Gram-negative, motile, mesophilic, violacein-producing bacteria were isolated from the soils and roots of Vaccinium macrocarpon Ait. and Kalmia angustifolia L. plants and from irrigation ponds associated with wild and cultivated cranberry bogs in Massachusetts, USA. Phylogenetic analyses of 16S rRNA gene sequences placed these isolates in a clade with Chromobacterium species, but the specialized environment from which they were isolated, their low genomic DNA relatedness with Chromobacterium violaceum ATCC 12472T and C. subtsugae PRAA4-1T, significant differences in fatty acid composition and colony morphology indicate that the cranberry and Kalmia isolates comprise a separate species of Chromobacterium , for which the name Chromobacterium vaccinii sp. nov. is proposed. Strain MWU205T ( = ATCC BAA-2314T = DSM 25150T) is proposed as the type strain for the novel species. Phenotypic analysis of 26 independent isolates of C. vaccinii sp. nov. indicates that, despite close geographical and biological proximity, there is considerable metabolic diversity among individuals within the population.

Chromobacterium haemolyticum-induced bacteremia in a healthy young man

“…The genus Chromobacterium consists of 7 recognized species: C. violaceum , C. subtsugae , C. aquaticum , C. haemolyticum , C. pseudoviolaceum , C. piscinae [1], and C. vaccinii [2]. Of these species, only C. violaceum, commonly found in the soil and water of tropical and subtropical regions, has been shown to cause human infection.…”

Isolation and Identification of Chromobacterium Sp. From Different Ecosystems

“…The pigments were produced in colonies but not secreted when cells were grown on agar media. Soby et al [23] discussed that the colonies were initially cream-colored, turning deep purple medial to a distal in the colony, the whole colony becoming deeply pigmented by the third day. Some isolates produce light purple color colonies.…”

“…This study mainly revealed the isolation of C. violaceum CV4 and new strain C. vaccinii CV5 in the Indian ecosystems. Previously this strain, C. vaccinii was discussed in the Soby et al [23], they isolated it from the irrigation ponds associated with the wild and cultivated cranberry bogs in Massachusetts, USA. Here, we also isolated this novel strain similar to those ecosystems (well water near paddy field from Nedumangad).…”

“…Here, we also isolated this novel strain similar to those ecosystems (well water near paddy field from Nedumangad). The key point is that the violacein-violet pigment produced by the various species is yet to be exploited and great potential in the various fields of cancer biology, biotechnological, industrial and clinical microbiology [23]. The selected two isolates would be used for …”

The genome of the insecticidal Chromobacterium subtsugae PRAA4-1 and its comparison with that of Chromobacterium violaceum ATCC 12472

“…More recently, an extract of C. subtsugae was approved by the EPA for use as an organic insecticide that is now commercially available as Grandevo® (Marrone BioInnovations Incorporated, Davis, CA, USA). Since the initial description of C. subtsugae , six additional species of Chromobacterium have been described: C. aquaticum , C. haemolyticum , C. piscinae , C. pseudoviolaceum , C. vaccinii , and C. amazonense [3], [4], [5], [6], [7]. The biology of these new species, and the metabolites they produce, remains largely unexplored.…”