Chromium (VI) Reduction by Cell Free Extract of Ochrobactrum anthropi Isolated from Tannery Effluent

Sultan, Sikander; Hasnain, Shahida

doi:10.1007/s00128-012-0648-1

Cited by 11 publications

(4 citation statements)

References 33 publications

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“…However, the psychrophilic temperature (e.g., 20 °C) was not beneficial to the Cr(VI) removal efficiency of O. anthropi YC152. The optimal pH and temperature for Cr(VI) removal by using O. anthropi observed in this study were consistent with those reported by Sultan and Hasnain (2012) [ 32 ]. Electrolytes, such as Na + and Cl − , exist at a high concentration in wastewater and can potentially affect the activity of microbes and the function of ion-exchange membranes in MFCs [ 23 ].…”

Section: Resultssupporting

confidence: 92%

“…Furthermore, Figure 4 B indicates that the other regression equation was determined to be y = −15.027 x + 156.8 ( r 2 = 0.9914) when the Cr(VI) concentration ranged from 0.3 to 5 mg/L. The different relationships observed in different Cr(VI) concentrations may be because of Cr(VI) reduction rates and bacterial characteristics [ 32 ]. Hence, on the basis of these two equations, the Cr(VI) concentration in the water samples can be rapidly determined using the developed MFC biosensor.…”

Section: Resultsmentioning

confidence: 99%

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Utility of Ochrobactrum anthropi YC152 in a Microbial Fuel Cell as an Early Warning Device for Hexavalent Chromium Determination

Wang¹,

Cheng

Liu

et al. 2016

Sensors

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Fast hexavalent chromium (Cr(VI)) determination is important for environmental risk and health-related considerations. We used a microbial fuel cell-based biosensor inoculated with a facultatively anaerobic, Cr(VI)-reducing, and exoelectrogenic Ochrobactrum anthropi YC152 to determine the Cr(VI) concentration in water. The results indicated that O. anthropi YC152 exhibited high adaptability to pH, temperature, salinity, and water quality under anaerobic conditions. The stable performance of the microbial fuel cell (MFC)-based biosensor indicated its potential as a reliable biosensor system. The MFC voltage decreased as the Cr(VI) concentration in the MFC increased. Two satisfactory linear relationships were observed between the Cr(VI) concentration and voltage output for various Cr(VI) concentration ranges (0.0125–0.3 mg/L and 0.3–5 mg/L). The MFC biosensor is a simple device that can accurately measure Cr(VI) concentrations in drinking water, groundwater, and electroplating wastewater in 45 min with low deviations (<10%). The use of the biosensor can help in preventing the violation of effluent regulations and the maximum allowable concentration of Cr(VI) in water. Thus, the developed MFC biosensor has potential as an early warning detection device for Cr(VI) determination even if O. anthropi YC152 is a possible opportunistic pathogen.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Utility of Ochrobactrum anthropi YC152 in a Microbial Fuel Cell as an Early Warning Device for Hexavalent Chromium Determination

Wang¹,

Cheng

Liu

et al. 2016

Sensors

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“…Most of reported Cr(VI)-reducing strains directly enzymatically reduce Cr(VI) to Cr(III) (Camargo et al 2003;Thacker et al 2007;Mistry et al 2010;Chen et al 2012;Focardi et al 2012). Chromate reductase activity is associated with the soluble cell fraction (Pal et al 2005;Sultan and Hasnain 2012) or membrane cell fraction (Wang et al 1990;Mangaiyarkarasi et al 2011). The purified soluble flavoproteins showed the activity of enzymatic Cr(VI) reduction in Pseudomonas putida and Escherichia coli (Ackerley et al 2004).…”

Section: Introductionmentioning

confidence: 94%

Bioreduction of Chromate by an Isolated Bacillus anthracis Cr-4 with Soluble Cr(III) Product

Jian

Liu

et al. 2015

Water Air Soil Pollut

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Microbial Cr(VI) reduction is a significant process in detoxification of Cr(VI) pollution. In this study, a new Cr(VI)-reducing bacterial strain, Cr-4, was isolated from soil around the chromiumcontaining slag. The analysis of the 16S ribosomal RNA (rRNA) gene sequence revealed that the newly isolated strain was closely related to Bacillus anthracis. The response to Cr(VI) stress and reduction capacity of the isolate were investigated. Cell growth decreased with the increase of Cr(VI) concentration. Cell morphology varied and cell growth was inhibited remarkably in the presence of 125 mg/L Cr(VI). The strain grew well and removed Cr(VI) effectively at a Cr(VI) concentration lower than 50 mg/L. Cr(VI)-reducing activity was inhibited by Zn 2+ , while significantly stimulated by Cu 2+ . The activity of Cr(VI) reduction by cell-free extract was demonstrated. Total chromium analysis and the energy-dispersive X-ray analysis (EDAX) spectrum revealed that Cr(VI) removal was caused mainly by microbial reduction rather than by biosorption and the main part of the reduced Cr(III) existed as soluble form in solutions.

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“…The routine abatement mechanism for chromium pollution generally involves reduction of Cr 6+ to Cr 3+ , and subsequent precipitation of less soluble chromium at or near neutral pH [8]. There are several methods for removal of chromium from the tanning wastewater.…”

Section: +mentioning

confidence: 99%

Microbial Treatment of Tannery Effluent by Augmenting Psychrotrophic Pseudomonas Putida Isolate

Garg¹,

Garg²,

Tripathi³

et al. 2018

EPP

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Abstract:In this study, Pseudomonas putida, a psychrotrophic isolate, has been employed for biological treatment of tannery effluent. The physicochemical parameters of tannery effluent were analysed. The dilution (1:1) of effluent, its supplementation with nutrients (e.g., glucose, yeast extract, peptone, each at 0.5%, w/v) and augmentation with P. putida test strain was done for bioremediation studies at natural pH 8.0. The efficient growth (OD 0.582), Cr 6+ removal (61.0%), decolorization (60.1%) and dechlorination (87.5%) were observed in diluted effluent during 96-120 h incubation in shake flask trial. Box-Behnkem design suggested simultaneous dechlorination (95.8%) and Cr 6+ removal (63.5%) in nutrients supplemented plus P. putida augmented only within 72 h incubation. The efficiency of bioremediation further enhanced in bioreactor. A direct correlation existed between chromate reductase activity and distribution of reduced chromium in different cell fractions and culture supernatant. Further enhanced dechlorination (68.2%) by 14.7% and Cr 6+ removal (72.7%) by 6.5% employing immobilized co-culture (P. putida and Bacillus cereus) as compared to bioremediation by single immobilized P. putida biomass. High extent of effluent bioremediation by immobilized biomass offer an attractive future of P. putida biomass for eco-friendly in situ bioremediation of tannery wastewater.

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Chromium (VI) Reduction by Cell Free Extract of Ochrobactrum anthropi Isolated from Tannery Effluent

Cited by 11 publications

References 33 publications

Utility of Ochrobactrum anthropi YC152 in a Microbial Fuel Cell as an Early Warning Device for Hexavalent Chromium Determination

Utility of Ochrobactrum anthropi YC152 in a Microbial Fuel Cell as an Early Warning Device for Hexavalent Chromium Determination

Bioreduction of Chromate by an Isolated Bacillus anthracis Cr-4 with Soluble Cr(III) Product

Microbial Treatment of Tannery Effluent by Augmenting Psychrotrophic Pseudomonas Putida Isolate

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