Chromium induced stress conditions in heterotrophic and auxotrophic strains of Euglena gracilis

Rocchetta, Iara; Mazzuca, Marcia; Conforti, Visitación; Balzaretti, V.; Molina, Marı́a del Carmen

doi:10.1016/j.ecoenv.2012.07.020

Cited by 16 publications

(9 citation statements)

References 52 publications

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“…Consistent with the result obtained, similar observations have also been reported in the literature regarding the influence of HMs on the lipidomic profile of certain microalgal strains (Rocchetta et al 2012(Rocchetta et al , 2006Sibi et al 2014). For instance, the lipid profiles of C. sorokiniana under Pb(II) stress increased the levels of C14, C16, and C18:1.…”

Section: Metabolomics Profiling Of Control and Pb(ii)-loaded Cellssupporting

confidence: 91%

Mechanisms of Lead (II) Phycoremediation Using Scenedesmus Obliquus: Cell Physicochemical Properties, Biochemical Composition and Metabolomic Profiling

Danouche

Aasfar

et al. 2021

Preprint

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This study highlights the mechanisms involved in Pb(II)-phycoremediation using the Pb(II) tolerant strain of Scenedesmus obliquus. Firstly, monitoring of cell growth kinetics in control and Pb(II)-doped medium revealed a significant growth inhibition, while the analyses through flow cytometry and Zetasizer revealed no difference in cell viability and size. Residual weights of control and Pb(II)-loaded cells assessed by thermogravimetric analysis, were 31.34% and 57.8%, respectively, indicating the uptake of Pb(II) into S. obliquus cells. Next, the use of chemical extraction to distinguish between the intracellular and extracellular uptake indicated the involvement of both biosorption (85.5%) and bioaccumulation (14.5%) mechanisms. Biosorption interaction of the cell wall and Pb(II) was confirmed by SEM, EDX, FTIR, zeta potential, zero-charge pH, and contact angle analyses. Besides, the biochemical characterization of control and Pb(II)-loaded cells revealed that the bioaccumulation of Pb(II) induces significant increases in the carotenoids and lipids content and decreases in the proportions of chlorophyll, carbohydrates, and proteins. Finally, the metabolomic analysis indicated an increase in the relative abundance of fatty acid methyl esters, alkanes, aromatic compounds, and sterols. However, the alkenes and monounsaturated fatty acids decreased. Such metabolic adjustment may represent an adaptive strategy, that preventing high Pb(II)-bioaccumulation in cellular compartments.

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Section: Metabolomics Profiling Of Control and Pb(ii)-loaded Cellssupporting

confidence: 91%

Mechanisms of Lead (II) Phycoremediation Using Scenedesmus Obliquus: Cell Physicochemical Properties, Biochemical Composition and Metabolomic Profiling

Danouche

Aasfar

et al. 2021

Preprint

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“…The pronounced increase in gill and digestive gland lipid peroxidation as well as the decreased humoral TOSC caused by starvation in EG mussels may be explained by the loss of such exogenous antioxidants upon starvation. Furthermore, an increase in lipid susceptibly to oxidation due to the incorporation of high amounts of unsaturated fatty acids from E. gracilis cells [74], may not be discarded.…”

Section: Oxidative Balancementioning

confidence: 99%

Long-term feeding with Euglena gracilis cells modulates immune responses, oxidative balance and metabolic condition in Diplodon chilensis (Mollusca, Bivalvia, Hyriidae) exposed to living Escherichia coli

Bianchi

Castro

Rocchetta

et al. 2015

Fish & Shellfish Immunology

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“…The primary target of heavy metals is suggested to be the PSII reaction center in chloroplasts and malfunctioning photosynthesis may be a source of increased ROS production (Rocchetta and Küpper 2009). Rocchetta et al (2012) showed that Euglena cells isolated from a polluted river had higher IC50 values for Cr 6+ , together with a higher GSH content and SOD activity than cells obtained from public culture collection indicating that the upregulation of antioxidative components is important for acclimation to harsh environments.…”

Section: Environmental Conditions and Oxidative Stress In Euglenamentioning

confidence: 98%

Biochemistry and Physiology of Reactive Oxygen Species in Euglena

Ishikawa¹,

Tamaki²,

Maruta³

et al. 2017

Advances in Experimental Medicine and Biology

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Reactive oxygen species (ROS) such as superoxide and hydrogen peroxide are by-products of various metabolic processes in aerobic organisms including Euglena. Chloroplasts and mitochondria are the main sites of ROS generation by photosynthesis and respiration, respectively, through the active electron transport chain. An efficient antioxidant network is required to maintain intracellular ROS pools at optimal conditions for redox homeostasis. A comparison with the networks of plants and animals revealed that Euglena has acquired some aspects of ROS metabolic process. Euglena lacks catalase and a typical selenocysteine containing animal-type glutathione peroxidase for hydrogen peroxide scavenging, but contains enzymes involved in ascorbate-glutathione cycle solely in the cytosol. Ascorbate peroxidase in Euglena, which plays a central role in the ascorbate-glutathione cycle, forms a unique intra-molecular dimer structure that is related to the recognition of peroxides. We recently identified peroxiredoxin and NADPH-dependent thioredoxin reductase isoforms in cellular compartments including chloroplasts and mitochondria, indicating the physiological significance of the thioredoxin system in metabolism of ROS. Besides glutathione, Euglena contains the unusual thiol compound trypanothione, an unusual form of glutathione involving two molecules of glutathione joined by a spermidine linker, which has been identified in pathogenic protists such as Trypanosomatida and Schizopyrenida. Furthermore, in contrast to plants, photosynthesis by Euglena is not susceptible to hydrogen peroxide because of resistance of the Calvin cycle enzymes fructose-1,6-bisphosphatse, NADP-glyceraldehyde-3-phosphatase, sedoheptulose-1,7-bisphosphatase, and phosphoribulokinase to hydrogen peroxide. Consequently, these characteristics of Euglena appear to exemplify a strategy for survival and adaptation to various environmental conditions during the evolutionary process of euglenoids.

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Chromium induced stress conditions in heterotrophic and auxotrophic strains of Euglena gracilis

Cited by 16 publications

References 52 publications

Mechanisms of Lead (II) Phycoremediation Using Scenedesmus Obliquus: Cell Physicochemical Properties, Biochemical Composition and Metabolomic Profiling

Mechanisms of Lead (II) Phycoremediation Using Scenedesmus Obliquus: Cell Physicochemical Properties, Biochemical Composition and Metabolomic Profiling

Long-term feeding with Euglena gracilis cells modulates immune responses, oxidative balance and metabolic condition in Diplodon chilensis (Mollusca, Bivalvia, Hyriidae) exposed to living Escherichia coli

Biochemistry and Physiology of Reactive Oxygen Species in Euglena

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