Chromium accumulation by two Streptomyces spp. isolated from riverine sediments

Amoroso, Maria Julia del R.; Castro, Guillermo R.; Duran, Ali; Peraud, Olivier; Oliver, G.; Hill, Russell T.

doi:10.1038/sj.jim.7000112

Cited by 58 publications

(35 citation statements)

References 25 publications

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“…AB0 grown in a copper supplemented MM were taken. Aliquots were removed for whole cell copper sorption analyses by nitric acid digestion while the remaining cells were broken in a French pressure cell at 20,000 psi (1,38 × 10 5 KN m -2 ) for sub-fractional sorption studies by differential centrifugation as described previously [8]. Samples of each fraction were removed for copper analysis.…”

Section: Subcellular Fractioningmentioning

confidence: 99%

“…Among the most common histochemical techniques, the Timm's reagent histochemical treatment has proved to be the most sensitive procedure for diagnosis of copper accumulation in liver when compared to orcein and rhodanine methods [24]. On the other hand, subcellular fractioning is a very common and valid technique for detecting metal distribution within the cell as it has been successfully applied to prove Cr and Cd accumulation in Streptomyces, Pseudomonas stutzeri and Bacillus subtilis, respectively [8,25,26].…”

Section: Introduction *mentioning

confidence: 99%

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Copper bioaccumulation by the actinobacterium Amycolatopsis sp. AB0

Albarracín

Winik

Kothe

et al. 2008

J. Basic Microbiol.

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Amycolatopsis sp. AB0, a copper resistant actinobacterium isolated from polluted sediments, has shown high copper specific biopsortion ability (25 mg g(-1)). Two approaches were used to confirm metal accumulation in growing cells of Amycolatopsis sp. AB0; we performed subcellular fractioning assays which showed that the retained copper was associated with the extra-cellular fraction (exopolymer, 40%), but mainly within the cells. Intracellular distribution of copper was: 86% in the cytosolic fraction, 11% at the cell wall and 3% associated with the ribosome/membrane fraction. Its copper bioaccumulation ability was corroborated by using silver enhanced staining of copper with the Timm's reagent technique, which has not been used to detect metal deposits in bacteria before. In addition, we constructed specific oligonucleotides for targeting genes coding for copper P-Type ATPases that could be involved in the copper uptake ability of this strain. A 607 bp DNA fragment was amplified and sequenced from Amycolatopsis sp AB0. BLAST search analysis showed 71% protein homology of the deduced sequence with a putative cation-transporting ATPase of Nocardia farcinica and 65% with a copper translocating ATPase of Mycobacterium flavescens. To our knowledge this is the first report of the presence of copper P-type ATPase genes in the Amycolotopsis genus.

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Section: Subcellular Fractioningmentioning

confidence: 99%

Section: Introduction *mentioning

confidence: 99%

Copper bioaccumulation by the actinobacterium Amycolatopsis sp. AB0

Albarracín

Winik

Kothe

et al. 2008

J. Basic Microbiol.

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“…The first report on Cr(VI) reduction by Streptomyces was by Das and Chandra [21]. Amoroso et al [22] reported on Cr(VI) bioaccumulation by Streptomyces strains, whereas Laxman and More [18] determined Cr(VI) reduction by Streptomyces griseus.…”

Section: Introduction *mentioning

confidence: 97%

Bioremediation of chromium(VI) contaminated soil by Streptomyces sp. MC1

Polti

García

Amoroso

et al. 2009

J. Basic Microbiol.

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This work provides quantitative information on Cr(VI) reduction in soil samples by an indigenous actinomycete. Streptomyces sp. MC1, previously isolated from sugarcane, has shown ability to reduce Cr(VI) in liquid minimal medium. A reduction of 100 and 75% was obtained at initial Cr(VI) concentrations of 5 and 50 mg l(-1), respectively, after 48 h of incubation. Bioremediation ability of Streptomyces sp. MC1 was assayed in soil extracts and soil samples. Relative growth of Streptomyces sp. MC1 was 77 and 38% when grown in soil extract with 10 and 50 mg l(-1) of Cr(VI), respectively. MC1 was able to reduce 30% of Cr(VI) after 96 h of incubation with 10 mg l(-1) of Cr(VI), and reduction coincided with the exponential growth phase at pH 7 and 30 degrees C.In soil samples, Streptomyces sp. MC1 was able to reduce up to 94% of the Cr(VI) bioavailability (50 mg kg(-1)) after 7 d. These results were compared with non-inoculated soil samples with Cr(VI). Bioremediation activity of Streptomyces sp. MC1 was not inhibited by natural soil microbial flora. Besides, Streptomyces sp. MC1 growth was not inhibited by 50 mg kg(-1) of Cr(VI). In contrast to findings obtained by other authors, our results showed almost complete Cr(VI) removal from soil without any previous treatment, and without addition of any substrate and with a normal soil humidity level. These results confirm the Cr(VI)-contaminated soil bioremediation potential of Streptomyces sp. MC1.

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“…The first report on Cr(VI) reduction by Streptomyces was documented by Das and Chandra (1990). Amoroso et al (2001), reported on Cr(VI) bioaccumulation by Streptomyces strains, whereas Laxman and More (2002) determined Cr(VI) reduction by Streptomyces griseus. Polti et al (2009), determined Cr(VI) reduction by Streptomyces sp.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance and Chromium Reduction Pattern Among Actinomycetes

Ali¹

2012

American Journal of Biochemistry and Biotechnology

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Actinomycetes, one of the most important groups of microbes, exhibit many interesting activities such as degradation and transformation of organic and metal substrates together with production of antibiotics. With these bioactivities, actinomycetes would play an important role in the webs of the marine environment. The present study was designed to evaluate the antibiotic resistance pattern, antibiotic producing potential and chromium resistance as well as chromium reduction potential of a range of actinomycetes isolated from marine environments. Actinomycetes were isolated from marine sediment samples obtained from St. Martin's Island in Bangladesh. Antibiotic resistance among the selected isolates was studied against 10 different antibiotics by disc diffusion method and antibiotic producing potential was assessed by the perpendicular streak method. The isolates were screened for resistance towards heavy metal Cr(VI) on culture plates supplemented with Cr(VI) at concentrations ranging from 1-5 mM of Cr(VI). Highly resistant isolates were subjected to screening for Cr(VI) reduction activity, which was estimated using the Cr(VI) specific colorimetric reagent 1, 5-diphenylcarbazide. Out of the total 30 different selected isolates, 25 (83.33%) showed resistance against more than three antibiotics and 6 (20%) showed resistance to more than six antibiotics. Ninety three percent of the isolates showed MAR index greater than 0.2 and tolerance to Cr(VI) at 1mM of initial Cr(VI). None of the isolates displayed antimicrobial activity against the organisms tested. Among the isolates tested for chromate reduction, two were most efficient showing complete reduction of 1mM Cr(VI) within 24 h. These two isolates were capable of reducing chromate even at high initial Cr(VI) concentrations. Remarkably, the isolate SM-11 was found to reduce 82.67%, 44.34% of Cr(VI) at 2.5mM, 5mM of initial Cr(VI) concentrations respectively, within 72h of incubation. The majority of the actinomycetes isolates displayed resistance to both antibiotics and heavy metal chromium which indicates the possible acquisition of resistance factors due to environmental or human activities. The study also demonstrates possible correlation between antibiotic resistance and metal tolerance. Two of the isolates which showed considerable chromium reduction activity even at high chromium concentrations, may find potential application in bioremediation approaches.

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Chromium accumulation by two Streptomyces spp. isolated from riverine sediments

Cited by 58 publications

References 25 publications

Copper bioaccumulation by the actinobacterium Amycolatopsis sp. AB0

Copper bioaccumulation by the actinobacterium Amycolatopsis sp. AB0

Bioremediation of chromium(VI) contaminated soil by Streptomyces sp. MC1

Antibiotic Resistance and Chromium Reduction Pattern Among Actinomycetes

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