CHROMATOGRAPHY ON TREATED FILTER PAPER<sup>1</sup>

“…However, both the colorimetric and spectrophotometric methods (i) are time consuming, (ii) make use of toxic or anyhow hazardous chemicals, (iii) require a strict control of both reaction time and temperature, since the instability of the reaction product may lead to low recoveries and wide statistical variations of the results and (iv) none of the methods is specific [3][4][5][7][8][9][10]12,[21][22][23]. Consequently, several chromatographic methods were developed that allow the determination of the individual carbonyls by paper chromatography [24,25], classical liquid-liquid partition chromatography [26], thin-layer chromatography [27], gas chromatography [28][29][30] or high-pressure liquid chromatography (HPLC) [22,31]. These methods are less time consuming, offer improved accuracy, sensitivity and specificity as compared to the colorimetric and spectrophotometric procedures and utilize less hazardous reagents [4,9,10,21].…”

Section: Introductionmentioning

confidence: 99%

High-performance liquid chromatographic determination of 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde in honey

Coco

Valentini²,

Novelli

et al. 1996

Journal of Chromatography A

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High performance liquid chromatographic determination of 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde in honey / LO COCO F.; VALENTINI C.; NOVELLI V.; CECCON L. -In: JOURNAL OF CHROMATOGRAPHY A. -ISSN 0021-9673. -749(1996), pp. 95-102. OriginalHigh performance liquid chromatographic determination of 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde in honey AbstractA method of determination of 2-furaldehyde (F) and 5-hydroxymethyl-2-furaldehyde (HMF) in honey by highperformance liquid chromatography (HPLC) is described. The method is based on the formation of the 2,4-dinitrophenylhydrazones of carbonyl compounds and subsequent reversed-phase separation of these derivatives. Derivatization is carried out by utilizing an acidic solution of 2,4-dinitrophenylhydrazine in acetonitrile. Precipitation of the derivatives of carbonyl compounds is thus avoided and direct injection of the sample into the HPLC system is allowed. The procedure offers a high specificity, since the derivatives of F and HMF are well separated from the other components of the mixture under examination. Recoveries of 95-99% were obtained from honey spiked at different levels with both analytes. The detection limit is of the order of 10/zg/kg of honey and reproducibility (mean of six determinations) is -+3% for F and +_2% tbr HMF.

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“…As Kirchner and Keller state (13), it is possible to wash excessively in removing chloride, and thereby to leach out too much of the silica. It has been said that the papers should be washed free from chloride (8).…”

Section: Methodsmentioning

confidence: 99%

On the Relation of White Blood Cells and Platelets to Venom Hemolysis

Turner

1956

The Journal of Experimental Medicine

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Removal of the white cells and platelets from suspensions of red cells usually produces substantial reduction in the hemolytic activity of venoms. Guinea pig red cells constitute a notable exception and may be lysed by a direct action of venom. White blood cells and platelets appear to contribute to hemolysis by serving as sources of phosphatides for the formation of lysophosphatides. No correlation could be found between phospholipase activity and direct hemolytic activity of venoms. A recently described method (8) of paper chromatographic separation of phospholipides has been used successfully in part of the work.

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CHROMATOGRAPHY ON TREATED FILTER PAPER¹

Cited by 66 publications

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Phospholipids. 3. On the chromatographic separation of glycerophospholipids

Phospholipids. 3. On the chromatographic separation of glycerophospholipids

High-performance liquid chromatographic determination of 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde in honey

On the Relation of White Blood Cells and Platelets to Venom Hemolysis

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CHROMATOGRAPHY ON TREATED FILTER PAPER1

Cited by 66 publications

References 0 publications

Phospholipids. 3. On the chromatographic separation of glycerophospholipids

Phospholipids. 3. On the chromatographic separation of glycerophospholipids

High-performance liquid chromatographic determination of 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde in honey

On the Relation of White Blood Cells and Platelets to Venom Hemolysis

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CHROMATOGRAPHY ON TREATED FILTER PAPER¹