Chromatography of Quorum Sensing Peptides: An Important Functional Class of the Bacterial Peptidome

Debunne, Nathan; Verbeke, Frederick; Janssens, Yorick; Wynendaele, Evelien; Spiegeleer, Bart De

doi:10.1007/s10337-017-3411-2

Cited by 16 publications

(14 citation statements)

References 120 publications

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“…The CSP is a peptide pheromone where the competence regulon engaged in the regulation of biofilm formation, stress response, and bacteriocin production is centered. To characterize the structure of peptides LC-MS method may be used (Wynendaele et al 2013;Verbeke et al 2017;Bikash et al 2018;Debunne et al 2018).…”

Section: Measurement Of Ai Productionmentioning

confidence: 99%

Prevention of biofilm formation by quorum quenching

Paluch

Rewak‐Soroczyńska

Jędrusik

et al. 2020

Appl Microbiol Biotechnol

274

151

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Quorum sensing (QS) is a mechanism that enables microbial communication. It is based on the constant secretion of signaling molecules to the environment. The main role of QS is the regulation of vital processes in the cell such as virulence factor production or biofilm formation. Due to still growing bacterial resistance to antibiotics that have been overused, it is necessary to search for alternative antimicrobial therapies. One of them is quorum quenching (QQ) that disrupts microbial communication. QQ-driving molecules can decrease or even completely inhibit the production of virulence factors (including biofilm formation). There are few QQ strategies that comprise the use of the structural analogues of QS receptor autoinductors (AI). They may be found in nature or be designed and synthesized via chemical engineering. Many of the characterized QQ molecules are enzymes with the ability to degrade signaling molecules. They can also impede cellular signaling cascades. There are different techniques used for testing QS/QQ, including chromatography-mass spectroscopy, bioluminescence, chemiluminescence, fluorescence, electrochemistry, and colorimetry. They all enable qualitative and quantitative measurements of QS/QQ molecules. This article gathers the information about the mechanisms of QS and QQ, and their effect on microbial biofilm formation. Basic methods used to study QS/QQ, as well as the medical and biotechnological applications of QQ, are also described. Basis research methods are also described as well as medical and biotechnological application.

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Section: Measurement Of Ai Productionmentioning

confidence: 99%

Prevention of biofilm formation by quorum quenching

Paluch

Rewak‐Soroczyńska

Jędrusik

et al. 2020

Appl Microbiol Biotechnol

274

151

View full text Add to dashboard Cite

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“…The process of quorum sensing is not only limited to bacteria, but it is also observed in other microbial cell types such as yeasts and fungi 2 , 3 . Different types of quorum sensing molecules in bacteria exist: Gram-negative bacteria mostly use N -acyl homoserine lactones, while Gram-positive bacteria produce oligopeptides for their communication, which are called quorum sensing peptides (QSPs) 4 – 6 . Other quorum sensing molecules, such as furan borate derivatives and other miscellaneous molecules, exist as well 7 , 8 .…”

Section: Introductionmentioning

confidence: 99%

“…They then interact with neighboring microbial cells via two possible mechanisms: either with membrane-located receptors (mainly histidine kinases) for signal transduction, or directly with cytoplasmic sensors (e.g. the RNPP family) after being transported over the microbial cell membrane by oligopeptide permeases 4 . The QSPs are chemically and microbiologically described in the Quorumpeps database which currently contains over 350 different peptides 9 .…”

Section: Introductionmentioning

confidence: 99%

PapRIV, a BV-2 microglial cell activating quorum sensing peptide

Janssens

Debunne

Spiegeleer

et al. 2021

Sci Rep

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Quorum sensing peptides (QSPs) are bacterial peptides produced by Gram-positive bacteria to communicate with their peers in a cell-density dependent manner. These peptides do not only act as interbacterial communication signals, but can also have effects on the host. Compelling evidence demonstrates the presence of a gut-brain axis and more specifically, the role of the gut microbiota in microglial functioning. The aim of this study is to investigate microglial activating properties of a selected QSP (PapRIV) which is produced by Bacillus cereus species. PapRIV showed in vitro activating properties of BV-2 microglia cells and was able to cross the in vitro Caco-2 cell model and reach the brain. In vivo peptide presence was also demonstrated in mouse plasma. The peptide caused induction of IL-6, TNFα and ROS expression and increased the fraction of ameboid BV-2 microglia cells in an NF-κB dependent manner. Different metabolites were identified in serum, of which the main metabolite still remained active. PapRIV is thus able to cross the gastro-intestinal tract and the blood–brain barrier and shows in vitro activating properties in BV-2 microglia cells, hereby indicating a potential role of this quorum sensing peptide in gut-brain interaction.

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“…Iodination of QSP with 125 I has already been performed to investigate the blood-brain barrier influx properties of some selected QSP; however, analytical quality information is lacking [6] . Moreover, Verbeke et al [7] give an overview of the currently used detection methods for unmodified QSP, while their chromatographic analysis is detailed by Debunne et al [8] .…”

Section: Introductionmentioning

confidence: 99%

Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry

Janssens

Debunne

Wynendaele

et al. 2018

Journal of Pharmaceutical Analysis

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Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C18 reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ionization (ESI) ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current (TIC) spectra. Non-iodinated peptides and mono- and di-iodinated peptides (NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low (2%) to high (57%).

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Chromatography of Quorum Sensing Peptides: An Important Functional Class of the Bacterial Peptidome

Cited by 16 publications

References 120 publications

Prevention of biofilm formation by quorum quenching

Prevention of biofilm formation by quorum quenching

PapRIV, a BV-2 microglial cell activating quorum sensing peptide

Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry

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