Chromatography of Proteins on Cellulose Ion-Exchangers

Chromatography of Serum Proteins in Normal and Pathologic Sera: The Distribution of Protein-Bound Carbohydrate and Cholesterol, Siderophilin, Thyroxin-Binding Protein, B12-Binding Protein, Alkaline and Acid Phosphatases, Radioiodinated Albumin and Myeloma Proteins

“…Cation-exchange cellulose columns have also been employed to fractionate human gamma globulin (31). These reports and the findings with anion-exchange cellulose chromatography serve to reemphasize the heterogeneity of materials normally present in the serum electrophoretic compartments.…”

Evidence that the Ciliary Inhibitor of Cystic Fibrosis is not an Antibody

“…Whole human serum was obtained from seven normal individuals whose serums did not inhibit oyster ciliary motion, 10 CF patients and 1 heterozygote whose serums inhibited ciliary activity. The IgG fractions were isolated by DEAE chromatography (12), starch block electrophoresis (13), and ammonium sulfate precipitation (14). The serum proteins present after fractionation were identified by immunoelectrophoresis (15).…”

“…Received for publication 3 April 1973 and in revised form 12 July 1973. 1 Abbreviations used in this paper: CF, cystic fibrosis; FITC, fluorescein isothiocyanate; F/P, fluorescein/protein; PBS, phosphate buffered saline; SRBC, sheep red blood cells.…”

Methacrylate‐based chromatographic media

“…However, these beads are mostly not suitable for the separations of biopolymers. The enormous popularity of ion exchange chromatography that has been introduced in the mid 1950s [6] gave birth to a very large number of commercial stationary phases built on a variety of supports including polysaccharides such as cellulose, dextran, and agarose, styrene-divinylbenzene, acrylamide, and methacrylate ester beads. Both HEMA and GMA-based supports are also amenable to a number of chemical modifications that lead to ion exchangers.…”