Chromatography of Proteins

The enzymic degradation of laminarin. 2. The multicomponent nature of fungal laminarinases

“…Irrigation was made at 1-2°by a descending flow of one of these solvent systems: (a) ammonium sulphate (30%, w/v), pH 6-8 (Simonart & Kwang Yti Chow, 1950); (b) acetone-water (1:1, v/v) (Jermyn, 1953); (c) sodium chloride (0-3m) in citrate buffer (0-0Sm), pH 5-6 (Gilligan & Reese, 1954). Protein was detected with Solvay purple (Jones & Michael, 1950) or bromothymol blue (Papastamatis & Wilkinson, 1951). Enzyme movement was followed by spraying the chromatogram with laminarin solution (0-5 %, w/v), incubating at 370 in a humidity chamber for 12 hr.…”

The proteins of Arachis hypogaea and fibre formation

“…They found it was possible, by means of ammonium sulphate fractionation of a sodium chloride extract of groundnut meal, to separate the globulins into two fractions-' arachin' and 'conarachin '-which have been found to differ in optical rotation and in the content of sulphur, basic nitrogen, lysine, methionine, cystine, threonine, tryptophan and tyrosine. Later Jones & Horn (1930) stated that arachin could be prepared from 10 % sodium chloride extract by dilution until the extract became cloudy, followed by saturation with carbon dioxide, or by the addition of 2 vol. of saturated ammonium sulphate to 3 vol.…”

Chromatographic Systems

“…These methods fall into two principal groups: those based upon physical properties and those based upon chemical properties. The former include fluorescence, radioactivity (21,27,43,60,75,122,145,152,198,216,226,233,245,374,380,384,385,391), photography and photoelectric methods (102,166,344), indicators used with organic acids (7), interferometry (102,166,344), and various visual methods including staining techniques (39,201). Chemical methods for streak or brush methods include many colored and color-producing reagents (248,419).…”