Chromatography of crotamiton and its application to the determination of active ingredients in ointments

Izumoto, Shin‐ichi; Machida, Yoshio; Nishi, Hiroyuki; Nakamura, Kouji; Nakai, Hideo; Satô, Tadashi

doi:10.1016/s0731-7085(96)02052-3

Cited by 18 publications

(9 citation statements)

References 6 publications

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“…An isocratic reversed-phase liquid chromatographic method was developed for determination of five active substances, including chlorhexidine, in an ointment using Zorbax RX-C 8 (4.6 mm × 150 mm, 5 m) column and a mobile phase containing sodium dodecyl sulfate and 2-propanol [13]. The hydrolytic pathway of chlorhexidine was investigated.…”

Section: Introductionmentioning

confidence: 99%

HPLC determination of chlorhexidine gluconate and p-chloroaniline in topical ointment

Havlíková

Matysová

Nováková

et al. 2007

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

HPLC determination of chlorhexidine gluconate and p-chloroaniline in topical ointment

Havlíková

Matysová

Nováková

et al. 2007

Journal of Pharmaceutical and Biomedical Analysis

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“…Several procedures have been reported in the literature for analysis of cinchocaine including spectrophotometry (Saleh and Askal, 1995;El-Gindy et al, 1998;Abdel-Ghani et al, 2005), fluorometry (Essig and Kovar, 2001), liquid chromatography (Padmanabhan, 1983;Izumoto et al, 1997), gas chromatography (Alkalay et al, 1981;Padmanabhan, 1983), thin-layer chromatography (Padmanabhan, 1983;Turan et al, 1989), polarography (Fernandez-Marcote et al, 1996) and potentiometric titration (European Pharmacopoeia, 2002), among others (Shirahama et al, 1983;Netesina et al, 1992;Cherkaoui and Veuthey, 1999;Okamoto et al, 2001). Development of suitable techniques for selective and sensitive determination of drugs in biological samples is of great interest, since the complexity of these samples, including large numbers of endogenous compounds, makes selective detection quite difficult.…”

Section: Cinchocainementioning

confidence: 99%

Rapid determination of cinchocaine in skin by high‐performance liquid chromatography

Elsayed

2007

Biomedical Chromatography

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A simple, rapid, accurate, precise and specific analytical method has been developed, validated and applied for determination of cinchocaine in guinea pig and albino rabbit dorsal skins, after in vivo application of cinchocaine formulations. Extraction was performed using a solvent mixture of ethanol and 0.1 M hydrochloric acid (90:10; v/v). Samples were chromatographed on Spheri-5, RP(18) column with a particle size of 5 microm and 220 mm x 4.6 mm i.d. The mobile phase was a mixture of acetonitrile and triethylamine phosphate buffer (pH 2.8; 0.04 M) (60:40, v/v). UV detection was carried out at 247 nm and the run time was 6 min with typical retention time of cinchocaine of 3.63 +/- 0.02 min. Specificity was demonstrated, showing that the cinchocaine peak was free of interference from skin endogenous components. The detector response was found to be linear in the concentration range 0.96-56.00 microg/mL with a coefficient of correlation r = 0.99996. The relative standard deviations of within- and between-day analyses were all below 5%. The drug extraction procedure was validated. Satisfactory recoveries with relative standard deviation values below 5% were obtained, indicating efficient quantitative reproducible extraction procedure.

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“…There are a few studies in the literature describing the determination of fluocortolone and its esters FCC and FCP using thin layer chromatography 122 [3] (TLC), high performance liquid chromatography [4][5] (HPLC), raman spectroscopy [6][7], and spectrophotometry [8]. Unlike fluocortolone, many studies including the use of spectrophotometry [2,9], fluorimetry [10], TLC [11], liquid chromatography-mass spectrometry [12], sequential injection chromatography [13], HPLC [2,[14][15][16], gas chromatography [17], chemometry [18] and electroanalytical methods [19][20][21] have been used for the determination of CIN. A HPLC method has been reported for the simultaneous determination of the three drugs [22].…”

Section: Introductionmentioning

confidence: 99%

Multivariate calibration method for Simultaneous estimation of Fluocortolone caproate, Fluocortolone pivalate and Cinchocaine HCl in suppository

Dönmez¹

2019

Studia UBB Chemia

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In this paper, the simultaneous quantitative assay of Fluocortolone caproate (FCC), Fluocortolone pivalate (FCP) and Cinchocaine HCl (CIN) in suppository has been reported as a simple alternative analytical method. A partial least squares (PLS-2) regression was used for resolution of the overlapped spectrophotometric signals from mixtures of the three drugs. A calibration set containing FCC, FCP and CIN was constructed by using 3 3 full factorial design in the concentration range of 4.5-8.5, 5-7 and 8-12 μg/mL, respectively. Absorption spectra were recorded in the range 240-350 nm. To check the quality of the proposed method, it was applied to the determination of these compounds in synthetic mixtures and pharmaceutical formulation, suppository. The PLS-2 method yielded recoveries ranging from 91 to 106%. The results compared well with those from the reported HPLC method.

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Chromatography of crotamiton and its application to the determination of active ingredients in ointments

Cited by 18 publications

References 6 publications

HPLC determination of chlorhexidine gluconate and p-chloroaniline in topical ointment

HPLC determination of chlorhexidine gluconate and p-chloroaniline in topical ointment

Rapid determination of cinchocaine in skin by high‐performance liquid chromatography

Multivariate calibration method for Simultaneous estimation of Fluocortolone caproate, Fluocortolone pivalate and Cinchocaine HCl in suppository

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