Chromatography Method

Interaction mechanism between TiO2 nanostructures and bovine leukemia virus proteins in photoluminescence-based immunosensors

“…[27][28][29] The gp51 is not a redox-protein therefore the charge transfer between gp51 and TiO 2 /glass is not possible. 30 But the gp51 protein, like many other proteins, contains a number of partially charged groups and domains, which in Fig. 3 and 4, are represented as partial charges "d-" and "d+".…”

Trends and challenges in microfluidic methods for protein manipulation—A review

“…Advantages of miniaturizing this technology are reduced sample and reagents consumption, fast analysis time, and improved separation efficiency [93]. An important aspect of LC is that it can be implemented through a wide variety of working principles (e.g., affinity, size, and charge), making it a quite complete technique suitable for different applications [74,75]. However, individually, the existence of several variations can make this technique very specific and intended only for a small part of the process where it is involved.…”

“…Chromatographic protein manipulation exploits physicochemical properties of the proteins such as net charge (ion-exchange chromatography [IEC]), or size and shape (size-exclusion chromatography [SEC]) [74,75]. The most used chromatographic method in analytical sciences is LC as it may be tailored to exploit all properties of proteins via high-performance liquid chromatography (HPLC), which can be combined with mass spectrometric detection [76,77].…”

“…Affinity chromatography exploits interactions (electrostatic, hydrophobic, van der Waals forces, and hydrogen bonding) between the affinity agents (immobilized affinity ligands) and biological molecules (analytes/targets, Figure S3A). For protein separation, affinity ligands include antibodies, aptamers, or other protein/peptide recognition molecules, which can be attached on a microfluidic channel surface [74,75]. Targeting low abundance analytes in complex fluids like blood and urine where proteins like IgG become interferents is a challenging task.…”