Chromatography Combined with Bioassays and Other Hyphenations – The Direct Link to the Compound Indicating the Effect

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

“…10 A number of microbiological and biochemical assays has been transferred to planar chromatography like HPTLC even though this field of research is still largely underexplored despite the evident advantage of linking directly to bioactive compounds. 3 …”

“…2 The process of separating components before biological analysis is also costly, requires great efforts and complex analytical system as well as database for the detection and identification of large number of substances. 3 Thus, the combination of efficient separation technique with bioassay seem to be the way forward and current advances made in the field of the application of bioassays in direct combination with chromatographic techniques has led to adequate characterization of unknown samples with respect to their profile of activity. [3] Bioautography, which refers to the hyphenation of chromatographic analysis with biological detection 4 can be done in both highly developed as well as small research laboratories with minimum sophisticated equipment.…”

“…3 Thus, the combination of efficient separation technique with bioassay seem to be the way forward and current advances made in the field of the application of bioassays in direct combination with chromatographic techniques has led to adequate characterization of unknown samples with respect to their profile of activity. [3] Bioautography, which refers to the hyphenation of chromatographic analysis with biological detection 4 can be done in both highly developed as well as small research laboratories with minimum sophisticated equipment. 5 The combination of bioassays, with separation methods and spectroscopic techniques has become a very attractive tool in the analysis of medicinal plant samples.…”

“…In the search for active compounds from highly complex sample mixtures such as in the field of natural product research, target analysis only is ineffective. 3,9 Combination of a database with high sophisticated hyphenated and comprehensive online methods are also not necessarily the key to the search for bioactive molecules as any disruption at a small link in the chain can force the whole process to a stop 3 thus reinforcing the need for bioassay-coupled chromatography. Bioassay-coupled chromatography also has the ability to reduce thousands of compounds in a complex mixture to a few important bioactive ones as well as reduce the rate of false positive seen in several experimental methods by complementing existing methodologies and providing specific information.…”

“…Bioassay-coupled chromatography also has the ability to reduce thousands of compounds in a complex mixture to a few important bioactive ones as well as reduce the rate of false positive seen in several experimental methods by complementing existing methodologies and providing specific information. 3 The application of hybrid chromatography in the analysis of plant samples have been discussed. 8 Characteristic points in the coupling process are bioassay, separation and detection.…”