Chromatography, Absorption, and Fluorescence: A New Instrumental Analysis Experiment on the Measurement of Polycyclic Aromatic Hydrocarbons in Cigarette Smoke

Wingen, L. M.; Low, Jason C.; Finlayson-Pitts, Barbara J.

doi:10.1021/ed075p1599

Cited by 17 publications

(14 citation statements)

References 15 publications

(22 reference statements)

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“…Tobacco smoke deposits were dissolved in a 1:1 mixture of methanol:methylene chloride. For BaP measurements the samples were filtered using a 0.2 µm Acrodisc CR (Gelman Sciences, Ann Arbor, MI) then evaporated to dry- 38 Recovery rates for spiked Pallflex filter samples and the hollow tracheobronchial airway model were similar and averaged 60.8 ± 1.7%. The recovery rate was low because of the multiple sequential containers that were required because of sample size requirements and because UVPM and BaP were measured from each sample.…”

Section: Model Preparationmentioning

confidence: 99%

Experimental and Numerical Smoke Carcinogen Deposition in a Multi-Generation Human Replica Tracheobronchial Model

Robinson

Oldham²,

Clinkenbeard

et al. 2006

Ann Biomed Eng

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A better understanding of submicron particle deposition in the respiratory tract is needed to study the health effects caused by carcinogenic particles. Recent studies indicate that random diffusion is not sufficient to describe the motion of these particles in complex geometries, rendering conventional models inaccurate. A solid replica of excised human lung segments was used to create digital and hollow models of the tracheobronchial region to investigate deposition of mainstream (MS) and sidestream (SS) cigarette smoke particles. Particle sizes for the carcinogen Benzo(a)pyrene (BaP) in SS smoke, and total particulate matter (UVPM) in SS and MS smoke were measured and used to compare the simulation to experimental data. Excellent agreement was found between predicted and measured results. Random diffusion was not found to be significant for submicron particles indicating that particles were instead transported to the airway wall by convective diffusion. BaP in SS smoke was an average 0.3 µm compared to 0.36 µm for UVPM in SS smoke. The trends in both experimental and numerical results indicated that the BaP in SS smoke deposits at a slightly higher efficiency than the UVPM, indicating that carcinogen-specific deposition, rather than total particulate matter should be considered when investigating health effects.

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Section: Model Preparationmentioning

confidence: 99%

Experimental and Numerical Smoke Carcinogen Deposition in a Multi-Generation Human Replica Tracheobronchial Model

Robinson

Oldham²,

Clinkenbeard

et al. 2006

Ann Biomed Eng

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“…Analysis of PAHs in TPM has been achieved by two major instrumental methods: high pressure liquid chromatography with fluorimetric detection (HPLC-FD) (15,(26)(27)(28) and gas chromatography-mass spectrometry (GC-MS) selective ion monitoring (SIM) mode analysis (16)(17)(18). Gas chromatography-flame ionization detection (GC-FID) and GC-MS have been employed for PAH analysis in gas phase of MSS (18,19).…”

Section: Determination Of Pahs In Mssmentioning

confidence: 99%

Distribution of Polycyclic Aromatic Hydrocarbons between the Particulate and the Gas Phase of Mainstream Cigarette Smoke in Relation to Cigarette Technological Characteristics

Kalaitzoglou¹,

Samara²

2005

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

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Particulate- and gas-phase polycyclic aromatic hydrocarbons (PAHs) were determined in the mainstream smoke (MSS) of 59 manufactured cigarette brands (commercially available brands of unknown tobacco and blend type) with variable ‘tar’ yields and physical/technological characteristics. Depending on the existence/absence of filter, the ‘tar’ yield indicated on the packet, and the cigarette length and diameter, the examined cigarette brands were classified into 15 groups: non filter (NF), high (H), medium (M), light (L), super light (SL), ultra light (UL), one-tar yields (O), 100 mm long cigarettes (H-100, L-100, SL-100, UL-100, O-100), and slim cigarettes (SL-SLIM, UL-SLIM, O-SLIM). Cigarettes were smoked in a reference smoking machine equipped with glass fibre filters for collection of PAHs bound to total particulate matter (TPM), and polyurethane foam plugs (PUF) for collection of gas-phase PAHs. The relationships of gas- and particulate-phase concentrations of PAHs (ng/cig) with the contents of typical MSS components, such as TPM, ‘tar’, nicotine and carbon monoxide were investigated. In addition, the phase partitioning of PAHs in MSS was evaluated in relation to the technological characteristics of cigarettes.

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“…These included gas chromatography [2,3], high-performance liquid chromatography (HPLC) [4][5][6] and hyphenated methods such as gas chromatography-mass spectrometry (GC-MS) [4,[5][6][7][8][9][10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Characterization of complex hydrocarbons in cigarette smoke condensate by gas chromatography–mass spectrometry and comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry

Zhao

Kong

Cai

et al. 2004

Journal of Chromatography A

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Chromatography, Absorption, and Fluorescence: A New Instrumental Analysis Experiment on the Measurement of Polycyclic Aromatic Hydrocarbons in Cigarette Smoke

Cited by 17 publications

References 15 publications

Experimental and Numerical Smoke Carcinogen Deposition in a Multi-Generation Human Replica Tracheobronchial Model

Experimental and Numerical Smoke Carcinogen Deposition in a Multi-Generation Human Replica Tracheobronchial Model

Distribution of Polycyclic Aromatic Hydrocarbons between the Particulate and the Gas Phase of Mainstream Cigarette Smoke in Relation to Cigarette Technological Characteristics

Characterization of complex hydrocarbons in cigarette smoke condensate by gas chromatography–mass spectrometry and comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry

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