Chromatographically pure fluorescein and tetramethylrhodamine isothiocyanates

Felton, Lloyd C.; McMillion, C. Robert

doi:10.1016/0003-2697(61)90068-9

Cited by 25 publications

(5 citation statements)

References 2 publications

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“…Fluorescein isothiocyanate; rather than the isocyanate, was chosen for thiv study because of its availability as a chromatographically pure crystalline product. 9 …”

Section: Tuuiumentioning

confidence: 99%

Chemical and Physical Variables Affecting Fluorescein Isothiocyanate and Its Protein Conjugates

Klugerman¹

1965

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“…Fluorescein isothiocyanate; rather than the isocyanate, was chosen for thiv study because of its availability as a chromatographically pure crystalline product. 9 …”

Section: Tuuiumentioning

confidence: 99%

Chemical and Physical Variables Affecting Fluorescein Isothiocyanate and Its Protein Conjugates

Klugerman¹

1965

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“…The high susceptibility of FITC to photobleaching (rapid fading subsequent to intense illumination) had since been improved ( 87 ) to sustain its popular usage to this day. At a maximum excitation of 498nm, FITC emits a green-coloured fluorescence (518nm); whilst another commonly used fluorochrome, tetramethylrhodamine isothiocyanate (TRITC) ( 88 , 89 ) emits a red-coloured fluorescence (582nm). The clear colour distinction between this fluorochrome pair has made them a common selection for two-colour fluorescent imaging.…”

Section: Ivm: the Associated Toolsmentioning

confidence: 99%

Diving head-first into brain intravital microscopy

Suthya,

Wong,

Bourne

2024

Front. Immunol.

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Tissue microenvironments during physiology and pathology are highly complex, meaning dynamic cellular activities and their interactions cannot be accurately modelled ex vivo or in vitro. In particular, tissue-specific resident cells which may function and behave differently after isolation and the heterogenous vascular beds in various organs highlight the importance of observing such processes in real-time in vivo. This challenge gave rise to intravital microscopy (IVM), which was discovered over two centuries ago. From the very early techniques of low-optical resolution brightfield microscopy, limited to transparent tissues, IVM techniques have significantly evolved in recent years. Combined with improved animal surgical preparations, modern IVM technologies have achieved significantly higher speed of image acquisition and enhanced image resolution which allow for the visualisation of biological activities within a wider variety of tissue beds. These advancements have dramatically expanded our understanding in cell migration and function, especially in organs which are not easily accessible, such as the brain. In this review, we will discuss the application of rodent IVM in neurobiology in health and disease. In particular, we will outline the capability and limitations of emerging technologies, including photoacoustic, two- and three-photon imaging for brain IVM. In addition, we will discuss the use of these technologies in the context of neuroinflammation.

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“…This relaxation time may be defined as the time required for the average value of cos () to change from unity (at time zero) to lie (at time e), where () is the angle between the directions of the emission oscillator at time zero and time (l. If the particles are spherical, then e becomes eo=3'fJV/RT, and Eq. [41] can be written [42] relating P also to the absolute temperature, T, and the viscosity, 'fJ, of the medium, and to the molar volume, V, of the spherical particle. Hence, a plot of (l/p -1/3) versus T/'fJ is linear with a slope of Rr/V and an intercept of (l/po -1/3).…”

Section: Polarization Of Fluorescencementioning

confidence: 99%