Chromatographic separation of tocopherols

Biochemical changes in phenols, flavonoids, tannins, vitamin E, β–carotene and antioxidant activity during soaking of three white sorghum varieties

“…The data obtained were subjected to one-way analysis of variance (ANOVA) and least significant difference (LSD) was at P<0.05. 30.38% for total phenols, total flavonoids and tannins content, respectively. Table 2 presented the changes in phenolic acids composition before and after soaking.…”

“…A volume of 0.250 mL was mixed with 0.250 mL Folin-Ciocalteau reagent and 0.50 mL of 10% sodium carbonate (Na 2 CO 3 ) and the volume was completed to 5 mL with distilled water. After incubation in dark at room temperature for 30 min, the absorbance of the reaction mixture was measured at 725 nm against blank. Gallic acid was used as a standard.…”

“…毬 -carotene was determined using the method outlined by Santra et al [30] . Samples (2 g) were added to watersaturated n-butanol and mixed by handshaking, and kept in the dark for [16][17][18] h for extraction of 毬 -carotene.…”

“…Samples (1 g) were extracted with 80% methanol (10 mL) for 2 h. Briefly, 2.4 mg of DPPH in 100 mL methanol was prepared and 3.9 mL of this solution was added to 0.1 mL of methanolic extract. The mixture was shaken vigorously and allowed to stand at room temperature for 30 min in the dark. Then the absorbance was measured at 515 nm.…”

Phytochemical Screening, Antioxidant Activities and In Vitro Anticancer Potential of Egyptian Capsicum Spp.

“…β-carotene was determined according to the method of Pupin et al [51]. Dried (5 g) pepper samples were extracted with ethyl acetate (3 × 50 ml) containing BHT (0.004%).…”