Chromatographic Separation of Proteins on A Pdms-Polymer Chip by Pressure Flow

Polymer Microchips Integrating Solid-Phase Extraction and High-Performance Liquid Chromatography Using Reversed-Phase Polymethacrylate Monoliths

“…Following the introduction of microfabricated silicon LC chips by Manz and co-workers in 1990, , research on microfabricated pressure-driven HPLC remained scarce until 2000, when Erickson et al demonstrated rapid anion-exchange LC separation of four model proteins within 1 min using a quartz HPLC microchip . Seki and colleagues later separated bovine serum albumin (BSA) and immunoglobulin G (IgG) by anion-exchange chromatography using a polydimethylsiloxane (PDMS) microchip, while Vahey et al fabricated a PDMS pressure-driven open tubular liquid chromatography (OTLC) microchip to analyze salt, indicator, and dyes, and Schlund et al reported an OTLC microchip driven by compressed air to separate phenols and vitamins . Following the development of high pressure on-chip valve and injector components by researchers at Sandia National Laboratories using an in situ photopolymerization technique, Reichmuth et al integrated these components for the development of an HPLC microchip enabling RPLC separations of peptides and proteins .…”

“…Significant efforts have focused on fabricating efficient chromatographic columns on pressure-driven HPLC microchips. Microfluidic open-tubular columns, ,, packed-bed columns, ,,,, micromachined pillar array columns, and continuous-bed or monolithic columns ,, have been reported. While open-tubular columns were the focus of initial chip-based HLPC development due to their low hydraulic resistance, moderate separation performance, and ease of fabrication, they suffer from low sample loading capacity resulting from limited total surface area.…”

Pressure-Driven Sample Injection with Quantitative Liquid Dispensing for On-Chip Electrophoresis

“…There are two major modes for introducing a sample liquid into the separation field: electrokinetic injection [2][3][4][5] and pressure-driven injection. [6][7][8] Electrokinetic injection is the most commonly used injection method for performing electrophoresis and electrochromatography on microfabricated devices. With this method, no mechanical devices are demanded, and injection is relatively easily achieved.…”

“…However, low reproducibility in the injection volume cannot be avoided using conventional methods on the nanoliter scale, even if the pressure is controlled in a precise manner. 6,8 In this study, we present a novel method for obtaining highly reproducible injection by pneumatic pressure and its application to on-chip electrophoresis.…”