Chromatographic separation of estrone and 17β-estradiol conjugates on DEAE-Sephadex

Hobkirk, R.; Musey, Paul I.; Nilsen, Mona

doi:10.1016/0039-128x(69)90033-6

Cited by 66 publications

(9 citation statements)

References 14 publications

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“…Material eluted in the methanol and methanol-water fractions (glucosiduronates) from the Florisil column was combined with additional estrone glucosiduronate, estradiol-17U3-3-glucosiduronate, and estradiol-17p-17-glucosiduronate standards and chromatographed with DEAE-Sephadex, using as eluent increasing concentrations of NaCl (0.1-0.2 mole/liter) in water in the form of a linear gradient (21). This system separates estrone and estradiol-17P glucosiduronates.…”

Section: Methodsmentioning

confidence: 99%

Metabolism of arterial plasma estrogens by the splanchnic organs of the dog in vivo

Collins¹,

Robinson²,

Howard³

et al. 1970

J. Clin. Invest.

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A B S T R A C T In order to study the splanchnic metabolism of blood-borne estrogens, a constant infusion of estrone-6,7-2H was made in a series of dogs, and arteriovenous (A-V) differences at equilibrium were determined for estrone-6,7-2H and for its products estradiol-17P, estrone estradiol-17,8 by the intestine, associated with substantial net production of estrone glucosiduronate. There is net uptake of estrone by the spleen and a small but significant net uptake of estrone glucosiduronate.

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Section: Methodsmentioning

confidence: 99%

Metabolism of arterial plasma estrogens by the splanchnic organs of the dog in vivo

Collins¹,

Robinson²,

Howard³

et al. 1970

J. Clin. Invest.

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“…Radioactive peaks corresponding to free steroids (15) were extracted into ether and in several instances submitted to a Girard reaction (16) followed by crystallization of ketonic and won-ketonic fractions with carrier El and Eu, respectively, both before and after acetylation (13). Well separated peaks (12,14) corresponding to E13S and E23S were incubated with mylase P (12) and extracted with ether. Crystallhatisn was performed as above.…”

Section: Bnvestigatio~~ Of Productsmentioning

confidence: 99%

17-Oxidoreduction of 17β-Estradiol, Estrone and Their 3-Sulfates by Kidney Slices from Guinea Pig and Human

Hobkirk¹,

Nilsen²,

Jennings³

1975

Can. J. Biochem.

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Slices of whole kidney and kidney cortex from the female guinea pig catalyzed a marked reduction of estrone 3-sulfate (E13S) and estrone (E1) to 17beta-estradiol 3-sulfate (E23S) and 17beta-estradiol (E2), respectively, as well as the reverse (dehydrogenation) reactions. Slices of medulla did not appear active in E23S-E13S interconversion but did possess the ability to interconvert E2 and E1, besides possessing considerable sulfatase activity. The use of [3H-55S]E13S and [3H-55S]E23S as substrates, together with a demonstrated lack of estrogen sulfate synthesis by the tissue slices, provided ample evidence that the intact sulfates were involved in direct oxidoreduction. Slices of human kidney cortex catalyzed the reduction of E13S to a very limited extent. Slices of whole kidney and of cortex from guinea pig formed small amounts of estrogen glucuronide(s).

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“…Guinea pig liver systems have been known for some time to be capable of forming estrogen glucuronides ( l , 2 ) and sulfates (3,4). Several in vivo studies in the same species suggested that little other than glucuronides were to be found in urine ( 5 , 6 ) or bile (5).…”

Section: Introductionmentioning

confidence: 96%

“…[4-14C]E3 with guinea pig liver slices and isolating the conjugate in question by DEAE-Sephadex (A25) chromatography (4). All labelled steroids and their conjugates were purified where necessary and stored for use as previously described (4,12).…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo studies on the metabolism of estrogens and their sulfates in guinea pigs

Hobkirk¹,

Freeman²,

Harvey³

et al. 1977

Can. J. Biochem.

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Labelled estradiol-17 beta(E2) or estrone (E1), when incubated with guinea pig liver slices, is metabolized by two main pathways. Part of each substrate is converted to estrone-3-glucuronide and estradiol-3-glucuronide. A further part of each is metabolized to estradiol-3-sulfate (E23S) and estrone-3-sulfate (E13S), which are interconverted. The latter conjugate appears to be the substrate for a 16 alpha-hydroxylase forming 16 alpha-hydroxyestrone-3-sulfate (16 alpha OHE13S). This, in turn, is further sulfurylated to yield 16 alpha-hydroxyestrone-3, 16-disulfate, accompanied by estriol-3,16-disulfate. A relatively small amount of tentatively indentified '6-hydroxyestrone disulfate accompanies these other two diconjugates. The guinea pig liver system suggests itself as a useful and relatively simple model for further study of 16 alpha-hydroxylation of E13S. The use of the latter as a natural subd E23S are present in liver, kidney, blood, gallbladder bile, intestine, uterus, and placenta after injection of labelled E2 into mature male and female guinea pigs. Some evidence has been obtained for the disulfate fraction (above) in liver and bile after injection of labelled E1.

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Chromatographic separation of estrone and 17β-estradiol conjugates on DEAE-Sephadex

Cited by 66 publications

References 14 publications

Metabolism of arterial plasma estrogens by the splanchnic organs of the dog in vivo

Metabolism of arterial plasma estrogens by the splanchnic organs of the dog in vivo

17-Oxidoreduction of 17β-Estradiol, Estrone and Their 3-Sulfates by Kidney Slices from Guinea Pig and Human

In vitro and in vivo studies on the metabolism of estrogens and their sulfates in guinea pigs

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