Chromatographic properties of macroporous beads from poly(GMA‐co‐EDMA)

Abstract

A series of macroporous glycidyl methacrylate-ethylene dimethacrylate copolymers have been prepared by the suspension polymerization according to a statistical design of experimental approach in which concentration of ethylene dimethacrylate and time needed for raising the polymerization temperature from 20 to 70 "C have been varied. The porous properties of hydrolyzed copolymers have been characterized by means of size exclusion chromatography using polystyrene standards in tetrahydrofuran. Pore volume, specific surface area, and pore size are affected by the crosslinking agent concentration rather than by the rate of temperature increase during the polymerization reaction. ZUSAMMENFASSUNG:Eine Reihe makroporoser Glycidylmethacrylat-Ethylendimethacrylat-Copolymerer (GMA-co-EDMA) wurde durch Suspensionspolymerisation hergestellt; dabei wurden nach einer statistisch geplanten experimentellen Durchfiihrung die Ethylendimethacrylatkonzentration und die Heizrate der Polymerisationstemperatur von 20 auf 70 "C variiert. Die Porositatseigenschaften der hydrolysierten Copolymeren wurden mit Hilfe der GroBenausschluBchromatographie unter Verwendung von Polystyrolstandards in Tetrahydrofuran charakterisiert. Das Porenvolumen, die spezifische Oberflache und die PorengroBe werden dabei durch die Konzentration des Vernetzungsmittels starker beeinflufit als durch die Heizrate wahrend der Polymerisation.

Polar Polymeric Stationary Phases for Normal-Phase HPLC Based on Monodisperse Macroporous Poly(2,3-dihydroxypropyl methacrylate-co-ethylene dimethacrylate) Beads

“…In contrast, much less is known about the effect of the kinetics of the polymerization that, in turn, depend on both the concentration and type of free-radical initiator and the polymerization temperature. [51][52][53][54][55][56] Our systematic work concerning the effect of temperature on the porous properties of beads prepared by "classical" suspension polymerization has demonstrated that temperature is probably the most convenient variable to regulate the pore size distribution of macroporous media since it allows for significant adjustment without requiring a change in the composition of the reaction mixture. 57 None of the previous studies mentioned above concerned monodisperse beads or provided a correlation between polymerization temperature and chromatographic performance of the resulting stationary phases.…”

“…Typically, the porous properties of macroporous beads are controlled by the type of porogen and the percentages of both porogen and cross-linker in the polymerization mixture. − These variables are closely related to the solubility of the polymer molecules in the polymerization system. In contrast, much less is known about the effect of the kinetics of the polymerization that, in turn, depend on both the concentration and type of free-radical initiator and the polymerization temperature. − Our systematic work concerning the effect of temperature on the porous properties of beads prepared by “classical” suspension polymerization has demonstrated that temperature is probably the most convenient variable to regulate the pore size distribution of macroporous media since it allows for significant adjustment without requiring a change in the composition of the reaction mixture …”

Methacrylate‐based chromatographic media

“…For example, poly(2,3-dihydroxypropyl methacrylate-co-EDMA) (DHPMA-EDMA) beads prepared by hydrolysis of epoxide groups of GMA-EDMA beads proved to be well suited for SEC in both aqueous and organic mobile phases [85,86,96]. The monodisperse version of these polar beads was expected to afford columns with enhanced efficiency, thus significantly increasing their value.…”

Three‐component terpolymers of glycidyl methacrylate with good swelling characteristics

“…For instance, GMA suspension copolymers are used as supports for enzymes [1][2][3] and as sorbents for chromatography. [4][5][6][7] They can also be used in removing metal ions or impurities from a solution (scavenger resins). [8][9][10][11][12][13][14] GMA polymers have been used as biomaterials as well, such as protein-separation and purification agents.…”

“…Further chemical modification of these groups opens a route to materials with diverse properties. For instance, GMA suspension copolymers are used as supports for enzymes1–3 and as sorbents for chromatography 4–7. They can also be used in removing metal ions or impurities from a solution (scavenger resins) 8–14.…”