Chromatographic procedures for the isolation of plant steroids

Dinan, Laurence; Harmatha, Juraj; Lafont, René

doi:10.1016/s0021-9673(01)00992-x

Cited by 134 publications

(82 citation statements)

References 130 publications

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“…The bands observed in diethyl ether and acetone extracts, respectively at 1751.3 and 1711.1 cm -1 , and at 1741.0 and 1712.5 cm -1 are responsible for the stretching vibration of C=D group, however no bands are shown for methanol extract. For all peels fruits extracts and for methanol seeds extract, the very strong absorptions observed at 3385.0/ 3370.5/3319.0 cm -1 and 3322.4 cm -1 for hydroxyl group (─DH) indicating the presence of withanolides in the sample (Dinan et al, 2001). Dnly for methanol seeds and peels fruits extracts, the presence of bands at 1593.6 and 1605.9 cm -1 respectively, provide the richness of methanol extract on protein.…”

Section: Ft-ir Spectral Analysis Of Extractsmentioning

confidence: 93%

Solvent extraction effects on phytochemical constituents profiles, antioxidant and antimicrobial activities and functional group analysis of Ecballium elaterium seeds and peels fruits

et al. 2017

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Ecballium elaterium is a perennial herb with multiple medicinal properties. It was widely used in folk medicine as cathartic, anti-inflammatory and analgesic agents. The present study was devoted to investigate the effect of diethyl ether, acetone, and methanol solvent on the extraction, phytochemicals profiles, antioxidant and antimicrobial activities of Ecballium elaterium seeds and peels fruits. The total phenolic, flavonoid, flavonol, condensed tannins and carotenoids contents were estimated. Maximum phenolic (107 ± 4 mg GAE/g) and flavonoid (18 ± 0 mg QE/g) contents were also found in the methanol peels fruits extract. Results showed that methanol peels fruits extract have the highest antioxidant activity with IC 50 values of 1.2 ± 0.1 and 1 ± 0 mg/mL for DPPH and ABTS, respectively, and EC 50 value of 1040 ± 5 mg/mL for reducing power assays. Acetone and diethyl ether peels fruits extracts showed the best antibacterial agents especially against Micrococcus luteus, however no antifungal activity was observed. Spectral data of FT-IR analysis of Ecballium elaterium seeds and peels fruits extracts revealed the presence of functional groups such as ─DH, C─H, C─D and C=D. Due to their high antioxidant and antibacterial activities, E. elaterium seeds and peels fruits extracts have promising potential as future natural antioxidant and antibacterial agents in food industry.Keywords: Ecballium elaterium; solvent effects; phytochemical contents; antioxidant and antimicrobial properties; FT-IR analysis.Practical Application: Ecballium elaterium seeds and peels fruits can be potentially used as natural antioxidant and antibacterial inhibitor.

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Section: Ft-ir Spectral Analysis Of Extractsmentioning

confidence: 93%

Solvent extraction effects on phytochemical constituents profiles, antioxidant and antimicrobial activities and functional group analysis of Ecballium elaterium seeds and peels fruits

et al. 2017

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“…On the other hand, the quan tities of solasonine decreased during fruit maturation in both D1 and J10 genotypes. To explain this phenomenon, it has been demonstrated that during fruit maturation, GAs like solasonine are converted into nitrogen-free and non-toxic composites (44). Finally, according to the recommended food safety level (200 mg per kg), although both D1 and J10 genotypes are recommendable for human food consumption, D1 is more suitable for human daily diet.…”

Section: Solasonine Measurementmentioning

confidence: 99%

Evaluation of Solasonine Content and Expression Patterns of SGT1 Gene in Different Tissues of Two Iranian Eggplant (Solanum melongena L.) Genotypes

Bagheri

Bushehri

Hassandokht

et al. 2017

Food Technol. Biotechnol.

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The family of Solanaceae (also known as nightshade family) is considered as a large plant family including essential crops such as potato, tomato, sweet pepper and lastly eggplant (1). In both tropical and temperate regions of the world, eggplant (Solanum melongena L., 2n=2x=24) has been cultivated for years, and regarded currently as an economically important vegetable plant (2). Phylogenetically, contrary to the other important Solanaceae fam- SummaryEggplant (Solanum melongena L.) is one of the most consumed vegetables in the world. The eggplant glycoalkaloids (GAs) are toxic secondary metabolites that may have detrimental effects on human health, particularly if the magnitudes of GAs are higher than the recommended food safety level (200 mg per kg of fresh mass). In this study, the content of solasonine compound and the expression patterns of solasodine galactosyltransferase (SGT1) gene were assessed in different tissues (mature leaves, flower buds, young, mature, and physiologically ripe fruits) of two Iranian eggplant genotypes (D1 and J10) under field conditions. The maximum mass fraction of solasonine in D1 was detected in flower buds (135.63 μg/g), followed by leaf (113.29 μg/g), physiologically ripe fruit (74.74 μg/g), young fruit (61.33 μg/g), and mature fruit (21.55 μg/g). Comparing both genotypes, the genotype of bitter fruits (J10) contained higher mass fraction of solasonine, as one of the main factors for producing bitter flavour of the plant. Regarding the expression profiles of SGT1, in both genotypes, the activity of the gene was increased nearly parallel with the concentration of solasonine. In the J10 geno type, transcript level of the gene was significantly higher than the genotype of sweet fruits (D1). Although both D1 and J10 genotypes are possibly recommendable for human food consumption, D1 is more suitable for daily diet.

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“…The rate at which the components of a mixture are separated depends on the activity of the adsorbent and polarity of the solvent. [5] In the past few years, gas chromatography-mass spectrum (GC-MS) analysis has become established as a key tool in secondary metabolite profiling. [6,7] GC separates volatile substances by percolating a gas stream over a stationary phase.…”

Section: Original Articlementioning

confidence: 99%

“…It was eluted sequentially with n-hexane and chloroform. The chloroform fraction was studied by GC-MS. [5] …”

Section: Isolation By Column Chromatographymentioning

confidence: 99%

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Mamillapalli¹

2017

AJP

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Aim: This investigation has been conducted to evaluate the antiasthmatic activity and phytochemical characterization using gas chromatography-mass spectrum (GC-MS) analysis of the leaf extracts of Talinum portulacifolium. Materials and Methods: Hydroalcoholic and acetone extracts of the plant were prepared. Preliminary phytochemical screening has been conducted. Antiasthmatic activity was determined by two experimental models. In vivo methods, histamine and acetylcholine (Ach)-induced bronchospasm in guinea pigs were studied. Pre convulsion time (PCT) was calculated. In vitro, experimental methods such as histamine and Ach-induced contractions in ileum were also studied. Percentage inhibition of contractions was calculated. Phytochemical characterization was studied using GC-MS analysis. Results and Discussion: In histamine and Ach-induced bronchospasm studies acetone extracts of the plant have significantly increased PCT 10.69 and 10.52 (**P < 0.01), one-way analysis of variance (ANOVA) Tukey's test compared with control. Histamine and Ach-induced ileum contraction studies also showed that the acetone extracts exhibited response 2.6 with 47% and 2.2 with 40% inhibition (*P < 0.05). The results were expressed by one-way ANOVA, Dunnett's test. The results of GC-MS analysis depicted following phytoconstituents with major peak area, namely, 79.29% methoxy-bis (cyclopentadiene), 2. 83% -5,10-dihexyl-5,10-dihydroindolo[3,2-b]indole-2,7-dicarbaldehyde, and 1.84% -1,2-bis[3,4-dimethoxy benzyl]-1,2-bis (methoxymethyl) ethane. Conclusion: The results of this study clearly indicate that the hydroalcoholic and acetone extracts of T. portulacifolium can be used as promising antiasthmatic agents. The activity may be due to the presence of phytochemicals reported through GC-MS.

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Chromatographic procedures for the isolation of plant steroids

Cited by 134 publications

References 130 publications

Solvent extraction effects on phytochemical constituents profiles, antioxidant and antimicrobial activities and functional group analysis of Ecballium elaterium seeds and peels fruits

Solvent extraction effects on phytochemical constituents profiles, antioxidant and antimicrobial activities and functional group analysis of Ecballium elaterium seeds and peels fruits

Evaluation of Solasonine Content and Expression Patterns of SGT1 Gene in Different Tissues of Two Iranian Eggplant (Solanum melongena L.) Genotypes

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