Chromatographic methods used for characterization of boswellic acids

This study was planned to develop a simple high-performance thin-layer chromatography method for qualitative and quantitative estimation of 3-acetyl-11-keto-β-boswellic acid (AKBBA), β-boswellic acid (BBA), 3-oxo-tirucallic acid (TCA) and serratol (SRT) with HPTLC-ESI-MS/MS for characterization in Boswellia serrata Roxb. oleo gum resin extract. The method was developed with hexane-ethyl acetate-toluene-chloroform-formic acid as mobile phase. RF values observed for AKBBA, BBA, TCA and SRT were 0.42, 0.39, 0.53 and 0.72, respectively. The method was validated according to International Council for Harmonisation guidelines. The concentration range for linearity was 100–500 ng/band for AKBBA and 200–700 ng/band for the other three markers with r2 > 0.99. The method resulted in good recoveries as 101.56, 100.68, 98.64 and 103.26%. The limit of detection was noticed as 25 , 37, 54 and 38 ng/band, with a limit of quantification as 76, 114, 116 and 115 ng/band, for AKBBA, BBA, TCA and SRT, respectively. The four markers were identified and confirmed in B. serrata extract using TLC-MS by indirect profiling by LC-ESI-MS/MS and were identified as terpenoids, TCA and cembranoids: AKBBA (mass/charge (m/z) = 513.00), BBA (m/z = 455.40), 3-oxo-tirucallic acid (m/z = 455.70) and SRT (m/z = 291.25), respectively.

show abstract

High Performance Thin Layer Chromatographic Method for The Simultaneous Quantification of Glimepiride and 3-Acetyl-11-Keto-Beta-Boswellic acid along with Robustness study using Fractional Factorial Design

Dalwadi,

Thakkar,

Shah

et al. 2023

RJPT

View full text Add to dashboard Cite

A simple, specific, and quantitative high-performance thin-layer chromatography method has been developed for the simultaneous determination of Glimepiride and 3-acetyl-11-keto-beta-boswellic acidin the formulation. Chromatographic development was performed using n-hexane: Ethyl acetate: Toluene: Glacial acetic acid (4:2:4:0.2, % v/v/v/v) as optimized mobile phase on TLC aluminum plate precoated withsilica gel 60 F254 and further densitometric measurement at 242nm.The drugs were satisfactorily resolved with Rfvalues of 0.38±0.02, 0.65±0.02, for Glimepiride and 3-acetyl-11-keto-beta-boswellic acid, respectively. The linear ranges were found to be 100-600 and 4000-24000ng/band for Glimepiride and 3-acetyl-11-keto-beta-boswellic acid. The accuracy and precision measured were less than 2% relative standard deviation for both drugs. The sensitivity of the method in terms of limit of detection and limit of quantitation were found to be 11.22 and 34ng/band for Glimepiride and 439.92 and 1333.102ng/band for 3-acetyl-11-keto-beta-boswellic acid respectively. 24-1 fractional factorial design was applied for robustness study to evaluate the small difference in independent variables. Among four factors, the significant factor found was the volume of n-hexane that resulted in a change in the Rf of drugs. The proposed method was found to be accurate, precise, reproducible, robust, and specific.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Chromatographic methods used for characterization of boswellic acids

Abstract: Different constraints affecting the quality of herbal drugs a) Generally, mixture of different constituents is present within the raw plant extract.

Cited by 3 publications

References 27 publications

In Vivo Antiinflammatory Activity of Facile Boswellic Acid Silver Nanoparticles and In Vitro Drug Release Kinetics

In Vivo Antiinflammatory Activity of Facile Boswellic Acid Silver Nanoparticles and In Vitro Drug Release Kinetics

A Simple HPTLC Approach of Quantification of Serratol and Tirucallic Acid with Boswellic Acids in Boswellia serrata by Validated Densitometric Method with MS/MS Characterization

High Performance Thin Layer Chromatographic Method for The Simultaneous Quantification of Glimepiride and 3-Acetyl-11-Keto-Beta-Boswellic acid along with Robustness study using Fractional Factorial Design

Contact Info

Product

Resources

About