Chromatographic methods for bile acid analysis

Street, Jackie; Setchell, Kenneth D.R.

doi:10.1002/bmc.1130020602

Cited by 32 publications

(14 citation statements)

References 159 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The derivatization of bile acids often involved two major reactions, the esterification of the carboxyl group and ether formation of the hydroxyl group as mentioned previously. Methyl, ethyl, n-propyl, n-butyl and isobutyl esters all had been used in the derivatization of carboxylic group for the GC analysis of bile acids [28,29]. Marschall et al [30] compared three different methods for derivatization: (i) diazomethane in diethyl ether, (ii) trimethylsilyldiazomethane in toluene and (iii) concentrated hydrochloric acid in dimethoxypropane for methylation of bile acids in GC-MS analysis.…”

Section: Optimization Of the Derivatization Condition For Bile Acidsmentioning

confidence: 99%

Determination of bile acids in pig liver, pig kidney and bovine liver by gas chromatography-chemical ionization tandem mass spectrometry with total ion chromatograms and extraction ion chromatograms

Tsai

Zhong

Weng

et al. 2011

Journal of Chromatography A

View full text Add to dashboard Cite

Section: Optimization Of the Derivatization Condition For Bile Acidsmentioning

confidence: 99%

Determination of bile acids in pig liver, pig kidney and bovine liver by gas chromatography-chemical ionization tandem mass spectrometry with total ion chromatograms and extraction ion chromatograms

Tsai

Zhong

Weng

et al. 2011

Journal of Chromatography A

View full text Add to dashboard Cite

“…A portion of each bile extract was subjected to HPLC analysis (18 Typical negative-ion FAB-MS spectra obtained from the bile of CF patients with associated liver disease, before (top spectrum) and after (bottom spectrum) UDCA administration. The following cholanoic acid structures are indicated from the ions: m/z 432 (glycomonohydroxy), m/z 448 (glyco-dihydroxy), m/z 464 (glyco-trihydroxy), m/z 480 (glyco-tetrahydroxy), m/z 498 (tauro-dihydroxy), m/z 514 (tauro-hydroxy), m/z 528 (glyco-dihydroxy-sulfate).…”

Section: Fast Atom Bombardment-mass Spectrometry Of Intactmentioning

confidence: 99%

Comprehensive Study of the Biliary Bile Acid Composition of Patients With Cystic Fibrosis and Associated Liver Disease Before and After Udca Administration

1990

View full text Add to dashboard Cite

The biliary bile acid composition was determined for patients with cystic fibrosis and associated liver disease before and after the administration of ursodeoxycholic acid (10 to 15 mg/kg body wt/day). Bile acids were analyzed by fast atom bombardment ionization-mass spectrometry, high performance liquid chromatography and gas chromatography-mass spectrometry after individual bile acids were separated according to their mode of conjugation using the lipophilic anion exchanger, diethylaminohydroxypropyl Sephadex LH-20. More than 50 individual bile acids were identified in the bile of cystic fibrosis patients and these acids were predominantly secreted as glycine and taurine conjugates. Small proportions (less than 8% of the total) of unconjugated and sulfate conjugates were present. Of interest was the identification of two side-chain-elongated (C25) bile acids, homocholic and homochenodeoxycholic acids. After ursodeoxycholic acid was administered, duodenal bile became enriched with the conjugated species of ursodeoxycholic acid (accounting for 11.9% to 32.5% of the total biliary bile acids), but to a lesser extent than reported previously for patients with other liver diseases or gallstones who received comparable doses of ursodeoxycholic acid, and this presumably occurs because of bile acid malabsorption that is a feature of cystic fibrosis. The mean glycine/taurine ratio increased from 2.4 before ursodeoxycholic acid administration to 5 after ursodeoxycholic acid administration even though these patients also received taurine. Despite the relatively low enrichment of the bile by ursodeoxycholic acid, biochemical indices of liver function all improved in these patients after ursodeoxycholic acid administration.

show abstract

“…There have been a number of reports on the liquid chromatographic separation, identification and quantification of bile acids in biological fluids (Swobodnik et al., 1987;Hasegawa et al, 1984;Li et al, 1989;Street and Setchell, 1989;Zhang et al, 1992;Chen ef al., 1992). At present, reversed phase high performance liquid chromatography (RP-HPLC) is the method of choice for the assay of free and conjugated bile acids in biological samples at elevated levels.…”

Section: Introductionmentioning

confidence: 97%

High performance liquid chromatographic determination of individual bile acids in serum for automatic diagnosis of various liver and biliary diseases

Zhao

Li²,

Chen

et al. 1993

Biomedical Chromatography

View full text Add to dashboard Cite

A reversed phase high performance liquid chromatographic method for the high sensitivity determination of individual bile acids in serum using a C18 column with a ternary solvent system combined with fluorometric techniques using immobilized enzymes is described. A computer-assisted diagnosis system using pattern recognition was developed to assist the clinical diagnosis of various liver and biliary diseases. A total consistency rate of 95% can be reached using this system.

show abstract

Chromatographic methods for bile acid analysis

Cited by 32 publications

References 159 publications

Determination of bile acids in pig liver, pig kidney and bovine liver by gas chromatography-chemical ionization tandem mass spectrometry with total ion chromatograms and extraction ion chromatograms

Determination of bile acids in pig liver, pig kidney and bovine liver by gas chromatography-chemical ionization tandem mass spectrometry with total ion chromatograms and extraction ion chromatograms

Comprehensive Study of the Biliary Bile Acid Composition of Patients With Cystic Fibrosis and Associated Liver Disease Before and After Udca Administration

High performance liquid chromatographic determination of individual bile acids in serum for automatic diagnosis of various liver and biliary diseases

Contact Info

Product

Resources

About