Chromatographic Methods for Analysis of the Metabolites of Acetophenetidin (Phenacetin)

Klutch, A.; Bordun, M.

doi:10.1002/jps.2600570337

Cited by 23 publications

(3 citation statements)

References 8 publications

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“…p-Hydroxyacetanilide-d3-p-Hydroxyaniline (535 mg, 5 mmoles), 12 M HCl (71 pl), deuterium oxide (8.43 ml)", 0.2 M sodium acetate (5 ml), acetic anhydride-d,j (510 mg, 468 pl, 5 mmoles)ll, and ether (10 ml) were combined in a 25-ml separator. The mixture was gently shaken every 5 min for 1 hr (8).…”

Section: Methodsmentioning

confidence: 99%

Quantitative Determination of Phenacetin and Its Metabolite Acetaminophen by GLC–Chemical Ionization Mass Spectrometry

Garland

Hsiao

Pantuck

et al. 1977

Journal of Pharmaceutical Sciences

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Section: Methodsmentioning

confidence: 99%

Quantitative Determination of Phenacetin and Its Metabolite Acetaminophen by GLC–Chemical Ionization Mass Spectrometry

Garland

Hsiao

Pantuck

et al. 1977

Journal of Pharmaceutical Sciences

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“…In addition, the urinary levels of presumed N-OHP metabolites reported were in no way an accurate reflection of the dose of N-OHP administered. There is only one report of the formation of N-OHP in a human after oral administration of P, and the method used for detection of N-OHP was not considered entirely satisfactory because of background interference (23). N-OHP was not identified in one of the two subjects given P, and only trace urinary amounts of N-OHP were identified in the other subject (23).…”

Section: By Vaught Andmentioning

confidence: 99%

“…There is only one report of the formation of N-OHP in a human after oral administration of P, and the method used for detection of N-OHP was not considered entirely satisfactory because of background interference (23). N-OHP was not identified in one of the two subjects given P, and only trace urinary amounts of N-OHP were identified in the other subject (23). N-OHP may at some point be definitively identified as a metabolite of P, other than by analogy with other aromatic amines.…”

Section: By Vaught Andmentioning

confidence: 99%

Phenacetin Safety

MACKLIN¹,

WELCH²

1980

Science

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Quantitative Bestimmung von Paracetamol im Blutserum durch HPLC mit Direktinjektion

Ebel

1980

Archiv der Pharmazie

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Es wird eine Bestimmung des Paracetamols durch HPLC mit fotometrischer Detektion beschrieben. Als einziger prachromatographischer Schritt wird eine EiweiBfallung so durchgefiihrt, daR keine Mitfallung des Arzneimittels erfolgt. Die Trennung erfolgt an reversed phase (RP8)-Fertigsiiulen. Die Erfassungsgrenze liegt bei etwa 100 ngiml, die Reproduzierbarkeit unter 3 % bei praktisch quantitativer Wiederfindungsrate. Etwa 15 % des Paracetamols liegen proteingebunden vor. Determination of Paracetamol in Blood Samples by HPLCA method is described for the determination of paracetamol (acetaminophen) in blood samples by HPLC and photometric detection. The only step before chromatography is the precipitation of plasma proteins without coprecipitation of the drug. For the separation reversed phase (RP8) material and ready made columns are used. The detection limit is about 100 ng/ml, the day to day precision is better than 3 %. Recovery is nearly quantitative. In blood serum, about 15 % of the paracetamol is bound to the plasma proteins.Paracetamol wird auRer in Monopraparaten vor allem in Kombination mit anderen ArzneisubstanZen wie z. B. Salicylamid, Coffein oder Pyrazolon-Derivaten arzneilich verwendet. Durch die Kombination mit anderen Arzneistoffen kann die Bioverfiigbarkeit des Paracetamols z. B. durch Konkurrenzreaktionen bei der Biotransformation beeinflufit werden. Im Rahmen von Untersuchungen zur Bioverfiigbarkeit der Paracetamols ist deshalb ein schnelles und wenig arbeitsintensives Verfahren zur Bestimmung der Konzentration im Serum von groBer Bedeutung. Wahrend friiher vorwiegend kolorimetrische Verfahren (Literatur vgl.')) angewendet wurden, wobei das auf Brodie

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Chromatographic Methods for Analysis of the Metabolites of Acetophenetidin (Phenacetin)

Cited by 23 publications

References 8 publications

Quantitative Determination of Phenacetin and Its Metabolite Acetaminophen by GLC–Chemical Ionization Mass Spectrometry

Quantitative Determination of Phenacetin and Its Metabolite Acetaminophen by GLC–Chemical Ionization Mass Spectrometry

Phenacetin Safety

Quantitative Bestimmung von Paracetamol im Blutserum durch HPLC mit Direktinjektion

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