Chromatographic immunoassays for Helicobacter pylori detection - are they reliable in Mali, West Africa?

Austarheim, Ingvild; Inngjerdingen, Kari Tvete; Paulsen, Berit Smestad; Togola, Adiaratou; Diakité, Chiaka; Diallo, Drissa

doi:10.11604/pamj.2013.14.72.2131

Cited by 3 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Surprisingly, an unusually low prevalence has been demonstrated in Mali where a rate of 21% was reported among persons with established gastric ulcer, 44% among the casually selected volunteers and 14% in those with gastrointestinal correlated illnesses. This low prevalence in the study population was attributed to the sensitivity of the detection kit [63] due to a possible H. pylori strain variability. Generally, the disease prevalence across countries in the West African area varies and there is no clear show of a steady overall increment or decrease with age.…”

Section: Resultsmentioning

confidence: 99%

Helicobacter pyloriInfection, Virulence Genes’ Distribution and Accompanying Clinical Outcomes: The West Africa Situation

Ofori

Adinortey

Bockarie

et al. 2019

BioMed Research International

View full text Add to dashboard Cite

Data on Helicobacter pylori (H. pylori) infection and virulence factors in countries across West Africa are scattered. This systematic review seeks to present an update on the status of H. pylori infection focusing on prevalence rate, distribution of virulent genes, and their link to clinical outcomes across countries in the western part of Africa. This information is expected to broaden the knowledge base of clinicians and researchers regarding H. pylori infection and associated virulence factors in West African countries. Search Method. A comprehensive search of the scientific literature in PubMed and ScienceDirect was conducted using the search terms including “Helicobacter pylori infection in West Africa”. Databases were sourced from January 1988 to December 2018. Results. Data on the incidence of H. pylori infection and related pathological factors were found for some countries, whereas others had no information on it. Smoking, alcohol, exposure to high levels of carcinogens and diet were reported to be involved in the pathogenesis of gastroduodenal diseases and gastric cancer. Besides the environmental factors and genetic characteristics, there are important characteristics of H. pylori such as the ability to infect, replicate, and persist in a host that have been associated with the pathogenesis of various gastroduodenal diseases. Concluding Remarks. This systematic search has provided information so far available on H. pylori virulence factors and clinical outcomes in West Africa. Accordingly, this piece has identified gaps in the body of knowledge highlighting the need for more studies to clarify the role of H. pylori virulence factors and associated clinical outcomes in the burden of this bacterial infection in West Africa, as data from these countries do not give the needed direct relation.

show abstract

Section: Resultsmentioning

confidence: 99%

Helicobacter pyloriInfection, Virulence Genes’ Distribution and Accompanying Clinical Outcomes: The West Africa Situation

Ofori

Adinortey

Bockarie

et al. 2019

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…In that report, Austarheim et al. found a sensitivity of 21% only for H. pylori stool antigen test in Mali in patients with gastric ulcer, although previous studies had reported 95% prevalence of H. pylori infection in Mali [21]. This lack of an appropriate gold standard for the diagnosis of H. pylori in Mali is therefore a limitation of this study.…”

Section: Discussionmentioning

confidence: 69%

“…In addition to this, it has been recently reported that the stool antigen test is a poor diagnostic test for the detection of H. pylori in Mali, which was not known when this study was conducted. In that report, Austarheim et al found a sensitivity of 21% only for H. pylori stool antigen test in Mali in patients with gastric ulcer, although previous studies had reported 95% prevalence of H. pylori infection in Mali[21]. This lack of an appropriate gold standard for the diagnosis of H. pylori in Mali is therefore limitation of this study.…”

mentioning

confidence: 66%

“…Both H. pylori and M. tuberculosis are widely endemic infections in Mali with a prevalence of 92 per 100 000 for TB and an estimated percentage of H. pylori infection ranging from 41 to 97% [2,21]. As co-infection with H. pylori and M. tuberculosis may confound results of the oral UBT, we evaluated the sensitivity and specificity of oral UBT to diagnose active TB and H. pylori in patients who were co-infected with H. pylori and M. tuberculosis, mono-infected with M. tuberculosis, and mono-infected with H. pylori.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Stool microbiome reveals diverse bacterial ureases as confounders of oral urea breath testing for Helicobacter pylori and Mycobacterium tuberculosis in Bamako, Mali

et al. 2016

View full text Add to dashboard Cite

Detection of bacterial urease activity has been utilized successfully to diagnose Helicobacter pylori (H. pylori). While Mycobacterium tuberculosis (M. tb) also possesses an active urease, it is unknown whether detection of mycobacterial urease activity by oral urease breath test (UBT) can be exploited as a rapid point of care biomarker for tuberculosis (TB) in humans. We enrolled 34 individuals newly diagnosed with pulmonary TB and 46 healthy subjects in Bamako, Mali and performed oral UBT, mycobacterial sputum culture and H. pylori testing. Oral UBT had a sensitivity and specificity (95% CI) of 70% (46–88%) and 11% (3–26%), respectively, to diagnose culture-confirmed M. tb disease among patients without H. pylori, and 100% sensitivity (69–100%) and 11% specificity (3–26%) to diagnose H. pylori among patients without pulmonary TB. Stool microbiome analysis of controls without TB or H. pylori but with positive oral UBT detected high levels of non-H. pylori urease producing organisms, which likely explains the low specificity of oral UBT in this setting and in other reports of oral UBT studies in Africa.

show abstract

“…As opposed to Asian countries that have a very high GC incidence, African countries have less subjects that develop GC. This phenomenon is called the "African enigma" [8][9][10][11][12]. In addition, the prevalence of infection and GC is affected by factors like age, environment, ethnicity, region of origin, smoking, alcohol consumption, immune status, diet, and the performance of diagnostic methods [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Helicobacter pylori infection in Bamako (Mali): Polymorphism, diversity and prophage

Guindo¹,

Yaro²,

Keita³

et al. 2023

GSC Biol. Pharm. Sci.

View full text Add to dashboard Cite

Helicobacter pylori (Hp) is a bacteria associated with gastritis and gastric cancer (GC). Its prevalence is higher than 80% in Mali and affects the incidence of GC, which is a major cancer in the department of digestive surgery. VacA and CagA toxins are responsible of Hp strain polymorphism. With prophage, they are also associated with a region depending diversity. The aim of this study was to identify the variants of Hp strains and their prophage identity in Bamako (Mali). A total of 61 gastric biopsies were isolated in patients with gastric diseases. The extracted DNA was amplified by PCR targeting cagA, vacA (s, m) and phage integrase (int) genes. cagA and int amplicons were sequenced according to Sanger method. Hp was detected in 23 (37.7%) samples. A predominance of housewives, commercial workers, farmers and students was observed, confirming the importance of socio-economic conditions and the associated factors in Hp infection. Carcinogenic vacAs1m1 was the second dominant polymorph. This would explain the prevalence of Hp's GC in the country. The phylogenetic tree from cagA sequences has revealed the specific variants strain of Hp that causes gastritis in Mali (HpML). Their prophage integration protein shares more than 90% of its sequence with species of the Schmidvirus genus. Like the HpML probable strains, Malian’s Hp prophage (HpMLpΦ) is geographically clustered with hspWestAfrica1 (hpAfrica1). These results confirm the existence of probable Hp and bacteriophage specific strains to Mali and suggest that using the molecular diagnostics method for effective pathogen identification and rapid patient management.

show abstract

Chromatographic immunoassays for Helicobacter pylori detection - are they reliable in Mali, West Africa?

Cited by 3 publications

References 15 publications

Helicobacter pyloriInfection, Virulence Genes’ Distribution and Accompanying Clinical Outcomes: The West Africa Situation

Helicobacter pyloriInfection, Virulence Genes’ Distribution and Accompanying Clinical Outcomes: The West Africa Situation

Stool microbiome reveals diverse bacterial ureases as confounders of oral urea breath testing for Helicobacter pylori and Mycobacterium tuberculosis in Bamako, Mali

Helicobacter pylori infection in Bamako (Mali): Polymorphism, diversity and prophage

Contact Info

Product

Resources

About