Chromatographic (GC-MS, HPLC) and virological evaluations of Salvia sclarea infected by BBWV-I

Hudaib, Mohammad; Bellardi, Maria Grazia; Rubies‐Autonell, C.; Fiori, Jessica; Cavrini, Vanni

doi:10.1016/s0014-827x(01)01038-2

Cited by 29 publications

(25 citation statements)

References 17 publications

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“…The composition of essential oils from healthy and infected S. sclarea plants was performed using GC-MS and HPLC analysis, as reported by Hudaib et al [18].…”

Section: Introductionmentioning

confidence: 99%

LC determination of citral in Cymbopogon citratus volatile oil

Rauber

Guterres

Schapoval

2005

Journal of Pharmaceutical and Biomedical Analysis

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“…The composition of essential oils from healthy and infected S. sclarea plants was performed using GC-MS and HPLC analysis, as reported by Hudaib et al [18].…”

Section: Introductionmentioning

confidence: 99%

LC determination of citral in Cymbopogon citratus volatile oil

Rauber

Guterres

Schapoval

2005

Journal of Pharmaceutical and Biomedical Analysis

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“…Temperature of the injector base, transfer line and ion source were maintained at 250, 250 and 2007C, respectively. System operation, temperature programs, qualitative and quantitative GC-MS analyses were all performed as reported in a previous paper [22].…”

Section: Gc-ms Systemmentioning

confidence: 99%

“…Identification of the different alkamide components of the lipophilic fraction was realized by a combination of RP-HPLC and GC-MS (on nonpolar column) analyses as described by Hudaib et al [22]. Briefly, the different alkamide fractions (compounds 1-9, Figs.…”

Section: Identification Of Alkamidesmentioning

confidence: 99%

“…The organic extract was evaporated and the residue was dissolved in 0.3 mL of methanol. This concentrated extract solution from each single fraction was simultaneously subjected to both the GC-MS [22] and the developed CD-MEKC analyses as a reference to confirm the peak identity in the total ion current (TIC)-GC-MS traces and CD-MEKC electropherograms of the tested samples, respectively. Moreover, for better confirmation of the GC-MS peaks corresponding to the smallest HPLC (and CE) peaks (minor alkamide fractions, e.g., 4, 5, 6, Figs.…”

Section: Identification Of Alkamidesmentioning

confidence: 99%

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Separation of alkamides fromEchinacea purpurea extracts by cyclodextrin-modified micellar electrokinetic chromatography

et al. 2002

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Separation of nine important alkyl methylbutyl- and isobutylamides (known as alkamides) obtained from Echinacea purpurea extracts was investigated by using cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC). Hydrophobic alkamides interact strongly with the micelles from the most common surfactants used in MEKC and this lead to predominant partition of the analytes into the micellar phase, resulting in poor resolution. The addition of neutral CDs to the alkaline (10 mM phosphate buffer pH 8.0) micellar system of sodium dodecyl sulfate (SDS), sodium cholate (SC) and sodium deoxycholate (SDC) was found to improve the separation of the studied alkamides. Among the several combinations surfactant/CD, three different systems showed to be particularly effective: SDS/hydroxypropyl-beta-CD (110 mM/100 mM) and SC/heptakis (2, 3, 6-tri-O-methyl)-beta-CD (200 mM/40 mM) which provided a complete separation of the studied compounds, and SDC/heptakis (2, 6-di-O-methyl)-beta-CD. The importance of appropriate surfactant vs. CD concentration ratio as well as that of total concentration of both surfactant and CD was considered. The optimization of the separation was performed by focussing the need for a rapid separation of nine alkamides diagnostically useful to define the fingerprint of Echinacea species.

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“…In clary sage, essential oil is obtained by steam distillation of fresh inflorescences in full bloom and mostly contains mono- and sesquiterpenes [3], [10]–[13]. Even though clary sage essential oil contains some sclareol, the main site of its accumulation is unknown and cannot be assumed to be within or outside the glandular trichomes.…”

Section: Introductionmentioning

confidence: 99%

Extracellular Localization of the Diterpene Sclareol in Clary Sage (Salvia sclarea L., Lamiaceae)

Caissard

Thomas

Delbecque³

et al. 2012

PLoS ONE

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Sclareol is a high-value natural product obtained by solid/liquid extraction of clary sage (Salvia sclarea L.) inflorescences. Because processes of excretion and accumulation of this labdane diterpene are unknown, the aim of this work was to gain knowledge on its sites of accumulation in planta. Samples were collected in natura or during different steps of the industrial process of extraction (steam distillation and solid/liquid extraction). Samples were then analysed with a combination of complementary analytical techniques (gas chromatography coupled to a mass spectrometer, polarized light microscopy, environmental scanning electron microscopy, two-photon fluorescence microscopy, second harmonic generation microscopy). According to the literature, it is hypothesized that sclareol is localized in oil pockets of secretory trichomes. This study demonstrates that this is not the case and that sclareol accumulates in a crystalline epicuticular form, mostly on calyces.

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Chromatographic (GC-MS, HPLC) and virological evaluations of Salvia sclarea infected by BBWV-I

Cited by 29 publications

References 17 publications

LC determination of citral in Cymbopogon citratus volatile oil

LC determination of citral in Cymbopogon citratus volatile oil

Separation of alkamides fromEchinacea purpurea extracts by cyclodextrin-modified micellar electrokinetic chromatography

Extracellular Localization of the Diterpene Sclareol in Clary Sage (Salvia sclarea L., Lamiaceae)

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