Chromatographic Fingerprinting as a Means of Quality Control: Distinction Between Actaea Racemosa and Four Different Actaea Species

Gafner, Stefan; Sudberg, Sidney; Sudberg, Elan M; Villinski, Jacquelyn R.; Gauthier, Robert; Bergeron, Chantal

doi:10.17660/actahortic.2006.720.8

Cited by 8 publications

(5 citation statements)

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“…Hence, it can be a useful tool for species identification of Actaea (Avula et al ., 2007). Recent reports from other groups have described methods for identification of species of Actaea based on TLC or HPTLC techniques (Gafner et al ., 2006; Reich et al ., 2008; Verbitski et al ., 2008). However, since TLC or HPTLC experiments often show lower selectivity and sensitivity than HPLC, the results from TLC analysis are less reliable.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical fingerprinting to thwart black cohosh adulteration: a 15 Actaea species analysis

Jiang

Motley

et al. 2011

Phytochemical Analysis

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Section: Resultsmentioning

confidence: 99%

Phytochemical fingerprinting to thwart black cohosh adulteration: a 15 Actaea species analysis

Jiang

Motley

et al. 2011

Phytochemical Analysis

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“…A study on two case reports demonstrated that the mechanism of liver injury due to black cohosh is idiosyncratic, leading to gradual hepatocellular disappearance [37]. However, it is suspected that some case reports may be due to the presence of an adulterant, specifically a species other than Actaea racemosa L. [38]. In a study from 2019, of 36 dietary supplements claimed to contain black cohosh (Actaea racemosa L.), nine were found to contain other Asian Actaea species (A. cimicifuga, A. dahurica, and A. simplex) rather than black cohosh, which may be hepatotoxic to humans [39].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Safety Concerns on Herbal Products with Assumed Phytoestrogenic Activity

et al. 2023

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Phytoestrogens (PEs) are plant-based compounds that can interact with estrogen receptors and are mainly used to treat menopausal complaints. However, the safety of products with assumed phytoestrogenic activity is not fully understood. This study aimed to identify plant species with assumed phytoestrogenic activity, review existing literature on their use and safety, and critically evaluate adverse reaction (AR) reports of single-herb, multi-herb, and mixed-multiple products, as submitted to the Netherlands Pharmacovigilance Centre Lareb and to VigiBase of the World Health Organization (WHO). In the Lareb database, the most commonly reported plant species to cause ARs (total of 67 reports) were Actaea racemosa L. (black cohosh) (47.8%), Humulus lupulus L. (hops) (32.8%), and Glycine max (L.) Merr. (soybean) (22.4%). In the VigiBase database (total of 21,944 reports), the top three consisted of Glycine max (L.) Merr. (71.4%), Actaea racemosa L. (11.6%), and Vitex agnus-castus L. (chaste tree) (6.4%). In the scoping review (total of 73 articles), Actaea racemosa L. (30.1%), Glycine max (L.) Merr. (28.8%), and Trifolium pratense L. (13.7%) were the most frequently mentioned plant species. ARs were most frequently reported in the system organ classes “gastrointestinal disorders”, “skin and subcutaneous tissue disorders”, “reproductive system and breast disorders”, and “general disorders and administration site conditions”. Furthermore, from the scoping review, it appeared that the use of products with assumed phytoestrogenic activity was associated with postmenopausal bleeding. It was concluded that, while the potential benefits of products with assumed phytoestrogenic activity have been extensively pursued, the potential occurrence of ARs after using these products is less well understood. This study highlights the need for further investigation and careful monitoring of these products to better understand their effects and ensure the safety and well-being of individuals using them.

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“…Although BCE exhibits genotoxic potential, the use of BCE should be considered in light of risk–benefit assessments. BCE is marketed to women to alleviate symptoms of gynecological ailments, suggesting that it may contain estrogenic constituents (Gafner ). However, BCE lacked estrogenic activity in animal studies conducted by the NTP (Mercado‐Feliciano et al ), and systematic reviews of clinical studies suggest that BCE is no more effective than placebo for alleviating symptoms of menopause (Laakmann et al ; Leach and Moore ; Franco et al ).…”

Section: Discussionmentioning

confidence: 99%

“…It is primarily marketed to older women with the stated intention of alleviating symptoms of menopause and is represented as an alternative to hormone replacement therapy. BCE is also used by younger women with the goal of alleviating symptoms of premenstrual syndrome (PMS) and to regulate the menstrual cycle (Gafner ).…”

Section: Introductionmentioning

confidence: 99%

Evidence for an Aneugenic Mechanism of Action for Micronucleus Induction by Black Cohosh Extract

Bernacki

Bryce

Bemis

et al. 2019

Environ and Mol Mutagen

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Black cohosh extract (BCE) is a popular botanical dietary supplement marketed to relieve symptoms of various gynecological ailments. Studies conducted by the National Toxicology Program (NTP) showed that BCE induces micronucleated erythrocytes in female rats and mice. Subsequently, the NTP showed that a variety of BCEs, including the sample that induced micronuclei (MN) in vivo (“NTP BCE”) had a similar effect in human TK6 cells. Further testing with the MultiFlow® DNA Damage Assay revealed that TK6 cells exposed to NTP BCE, as well as a BCE reference material (BC XRM), exhibited a signature consistent with aneugenic activity in TK6 cells. Results from experiments reported herein confirmed these in vitro observations with NTP BCE and BC XRM. We extended these studies to include a novel test system, the MultiFlow Aneugen Molecular Mechanism Assay. For these experiments, TK6 cells were exposed to NTP BCE and BC XRM over a range of concentrations in the presence of fluorescent Taxol (488 Taxol). After 4 h, nuclei from lysed cells were stained with a nucleic acid dye and labeled with fluorescent antibodies against phospho‐histone H3 (p‐H3) and Ki‐67. Whereas BCEs did not affect p‐H3:Ki‐67 ratios (a signature of aneugenic mitotic kinase inhibitors), 488 Taxol‐associated fluorescence (a tubulin binder‐sensitive endpoint) was affected. More specifically, 488 Taxol‐associated fluorescence was reduced over the same concentration range that was previously observed to induce MN. These results provide direct evidence that BCEs destabilize microtubules in vitro, and this is the molecular mechanism responsible for the aneugenicity findings. Environ. Mol. Mutagen. 2019. © 2019 The Authors. Environmental and Molecular Mutagenesis published by Wiley Periodicals, Inc. on behalf of Environmental Mutagen Society.

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Chromatographic Fingerprinting as a Means of Quality Control: Distinction Between Actaea Racemosa and Four Different Actaea Species

Cited by 8 publications

References 0 publications

Phytochemical fingerprinting to thwart black cohosh adulteration: a 15 Actaea species analysis

Phytochemical fingerprinting to thwart black cohosh adulteration: a 15 Actaea species analysis

Analysis of Safety Concerns on Herbal Products with Assumed Phytoestrogenic Activity

Evidence for an Aneugenic Mechanism of Action for Micronucleus Induction by Black Cohosh Extract

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