Chromatographic Fingerprint analysis for Determining the Presence of Vitexin and Isovitexin on Seven Varieties of Ficus Deltoidea

Abstract

Ficus deltoidea Jack or locally known as mas cotek from the family of Moraceae has been acknowledged for its occurrence of several varieties based on the variations of leaf morphology, namely F. deltoidea var. angustifolia (Miq.) Corner, F. deltoidea var. bilobata Corner, F. deltoidea var. kunstleri (King) Corner, F. deltoidea var. lutescens (Desf.) Corner, F. deltoidea var. trengganuensis Corner, F. deltoidea var. motleyana and F. deltoidea var. deltoidea Jack. In this work, we report on the chemical profiles using chromatographic techniques on the seven varieties of F. deltoidea by analysing the presence of the bioactive compounds, vitexin and isovitexin and characterising the other major peaks in the chromatograms. Observation on the HPLC chromatogram showed the presence of both compounds in all samples except FDv. K (vitexin only) and FDv. I. Other peaks showed a characteristic of flavonoids based on the UV spectra pattern with UV maxima at 270 and 338 nm except for peaks 6, 7 and 8 with UV maxima at 294, 319 and 210, respectively. Both reference compounds were presence in varies concentration in all samples analysed.