Chromatographic evaluation of perfusion on charcoal in uraemia

Perfusion of Uremic Blood Ultrafiltrate on Uncoated Charcoal

“…Inexpensive uncoated granular charcoal could be the appropriate complement to improve the efficiency of dialysis. The charcoal is able to remove most of the compounds present in the ultrafiltrate, whose chromatograms downstream charcoal, either by gel chromatography or by HPLC are quite flat, as reported elsewhere (14). During charcoal treatment, chromatograms of ultrafiltrates sampled at the beginning of each subsequent session appear to be constantly decreased and reach stable and low values after 16 treatments as reported both in Fig.…”

“…This simple technique is an acceptable screening method when the goal is to obtain a "fingerprint" (i.e., a snapshot) of the humoral situation of the uremic patient and not the separation or the identification of "uremic toxins." Biogel P2 chromatography gives a very poor pattern which, however, when considered as a whole, does not offer less information than those obtained by the richer high-performance liquid chromatography (HPLC) pattern as remarked in a previous technical chromatographic report (14).…”