Chromatographic determination of impurity binding affinities on biomineral crystals

Mazzarino, Randall C.; Thomas, Tissa J.; Sours, Ryan E.

doi:10.1039/c0ce00454e

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…The investigation of adsorption properties of biomolecules on these materials is important due to their relevance to biomineralization processes. 42 Furthermore, application of these materials as the stationary phase in chromatography allows to perform separation of biomolecules (e.g., peptides and proteins) under mild separation conditions, which reduces the risk of their destruction (e.g., denaturation) and provides higher recovery of bioactivity. 37 This way, alternative materials to the common silica-/alumina-based materials for TLC could be introduced broadening the application range and enabling to directly test interactions of, for example, proteins involved in bone formation with hydroxyapatitethe main constituent of bone.…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies show that CaCO 3 with hierarchical structures can be developed as new stationary phases for chromatography. , Calcium phosphate materials, particularly hydroxyapatite, have been used as columns for protein separation and purification. , In this work, nanosized Mg-ACC, MCSH, and HAp were applied as the stationary phases and water as the solvent. The investigation of adsorption properties of biomolecules on these materials is important due to their relevance to biomineralization processes . Furthermore, application of these materials as the stationary phase in chromatography allows to perform separation of biomolecules (e.g., peptides and proteins) under mild separation conditions, which reduces the risk of their destruction (e.g., denaturation) and provides higher recovery of bioactivity .…”

Section: Introductionmentioning

confidence: 99%

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Inorganic Porous Bulk Discs as a Matrix for Thin-Layer Chromatography and Translucent Hard Composite Materials

Chen

Knaus

Luo

et al. 2019

ACS Appl. Mater. Interfaces

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Magnesium-stabilized amorphous calcium carbonate (Mg-ACC), amorphous magnesium calcium silicate hydrate (MCSH), and hydroxyapatite (HAp) are prepared by a precipitation method. By cold-pressing these particles, it is possible to produce porous bulk discs with a narrow pore size distribution. These porous inorganic discs (Mg-ACC, MCSH, and HAp) are investigated as stationary phases to study the chromatographic behavior and adsorption ability of rhodamine B, methylene blue, and ribonuclease. The adsorption affinities of different biomolecules can be easily observed and evaluated through this method. Furthermore, by infiltrating fabricated opaque porous discs with benzyl ether, which has a similar refractive index as the used inorganic particles (Mg-ACC, MCSH, and HAp), their optical properties significantly change and the discs become translucent. Moreover, by infiltrating the MCSH discs with a light-curing polymer, translucent composites with good surface hardness are fabricated. By doping particles with ions such as Ni2+, Co2+, Fe3+, and Eu3+, the color and UV–visible spectrum of the bulk discs can be adjusted. Typically, by using iron-doped MCSH particles as the inorganic matrix, nanocomposites, which show a steep UV-absorption edge at 400 nm, are fabricated. Our work provides a simple and economical method to evaluate the affinity of biomolecules to inorganic materials and a novel way to fabricate translucent hard composite materials. The fabricated nanocomposite discs show a great UV shielding effect and superior surface hardness compared to polymethyl methacrylate and commercial sunglasses, suggesting their potential as new sunglass materials.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inorganic Porous Bulk Discs as a Matrix for Thin-Layer Chromatography and Translucent Hard Composite Materials

Chen

Knaus

Luo

et al. 2019

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

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Hierarchical CaCO₃ Chromatography: A Stationary Phase Based on Biominerals

Sato

Oaki

Takahashi

et al. 2015

Chemistry A European J

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In biomineralization, acidic macromolecules play important roles for the growth control of crystals through a specific interaction. Inspired by this interaction, we report on an application of the hierarchical structures in CaCO3 biominerals to a stationary phase of chromatography. The separation and purification of acidic small organic molecules are achieved by thin-layer chromatography and flash chromatography using the powder of biominerals as the stationary phase. The unit nanocrystals and their oriented assembly, the hierarchical structure, are suitable for the adsorption site of the target organic molecules and the flow path of the elution solvents, respectively. The separation mode is ascribed to the specific adsorption of the acidic molecules on the crystal face and the coordination of the functional groups to the calcium ions. The results imply that a new family of stationary phase of chromatography can be developed by the fine tuning of hierarchical structures in CaCO3 materials.

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Chromatographic determination of impurity binding affinities on biomineral crystals

Cited by 2 publications

References 25 publications

Inorganic Porous Bulk Discs as a Matrix for Thin-Layer Chromatography and Translucent Hard Composite Materials

Inorganic Porous Bulk Discs as a Matrix for Thin-Layer Chromatography and Translucent Hard Composite Materials

Hierarchical CaCO₃ Chromatography: A Stationary Phase Based on Biominerals

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Chromatographic determination of impurity binding affinities on biomineral crystals

Cited by 2 publications

References 25 publications

Inorganic Porous Bulk Discs as a Matrix for Thin-Layer Chromatography and Translucent Hard Composite Materials

Inorganic Porous Bulk Discs as a Matrix for Thin-Layer Chromatography and Translucent Hard Composite Materials

Hierarchical CaCO3 Chromatography: A Stationary Phase Based on Biominerals

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Hierarchical CaCO₃ Chromatography: A Stationary Phase Based on Biominerals