Chromatographic Determination of Carbonyl Compounds as Their 2,4-Dinitrophenylhydrazones II. High Pressure Liquid Chromatography

Papa, Louis J.; Turner, L. P.

doi:10.1093/chromsci/10.12.747

Cited by 75 publications

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“…When the reaction was completed (carbohydrates were in the solution), the formaldehyde concentration was determined colorimetrically by the reaction with chromotropic acid (Poludek-Fabini and Beirikh, 1981). Carbohydrates were HPLC analyzed with a Milichrom A-02 microcolumn chromatograph (Russia) using a preliminary derivatization with dinitrophenylhydrazine (Papa and Turner, 1972;Cherstiouk et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Putative mechanism of the sugar formation on prebiotic Earth initiated by UV-radiation

Pestunova

Simonov

Снытников

et al. 2005

Advances in Space Research

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Section: Discussionmentioning

confidence: 99%

Putative mechanism of the sugar formation on prebiotic Earth initiated by UV-radiation

Pestunova

Simonov

Снытников

et al. 2005

Advances in Space Research

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“…However, both the colorimetric and spectrophotometric methods (i) are time consuming, (ii) make use of toxic or anyhow hazardous chemicals, (iii) require a strict control of both reaction time and temperature, since the instability of the reaction product may lead to low recoveries and wide statistical variations of the results and (iv) none of the methods is specific [3][4][5][7][8][9][10]12,[21][22][23]. Consequently, several chromatographic methods were developed that allow the determination of the individual carbonyls by paper chromatography [24,25], classical liquid-liquid partition chromatography [26], thin-layer chromatography [27], gas chromatography [28][29][30] or high-pressure liquid chromatography (HPLC) [22,31]. These methods are less time consuming, offer improved accuracy, sensitivity and specificity as compared to the colorimetric and spectrophotometric procedures and utilize less hazardous reagents [4,9,10,21].…”

Section: Introductionmentioning

confidence: 99%

“…This allows the quantitation of trace amounts of carbonyls in several matrices [22,23,31]. The most widely utilized derivative is the corresponding 2,4-dinitrophenylhydrazone (DNPH-one); in fact, (i) the reaction between 2,4-dinitrophenylhydrazine (DNPH) and carbonyl compounds is highly specific and (ii) the use of DNPH as the reagent provides a rather rapid sample preparation, thus minimizing background effects [32].…”

Section: Introductionmentioning

confidence: 99%

High-performance liquid chromatographic determination of 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde in honey

Coco

Valentini²,

Novelli

et al. 1996

Journal of Chromatography A

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High performance liquid chromatographic determination of 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde in honey / LO COCO F.; VALENTINI C.; NOVELLI V.; CECCON L. -In: JOURNAL OF CHROMATOGRAPHY A. -ISSN 0021-9673. -749(1996), pp. 95-102. OriginalHigh performance liquid chromatographic determination of 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde in honey AbstractA method of determination of 2-furaldehyde (F) and 5-hydroxymethyl-2-furaldehyde (HMF) in honey by highperformance liquid chromatography (HPLC) is described. The method is based on the formation of the 2,4-dinitrophenylhydrazones of carbonyl compounds and subsequent reversed-phase separation of these derivatives. Derivatization is carried out by utilizing an acidic solution of 2,4-dinitrophenylhydrazine in acetonitrile. Precipitation of the derivatives of carbonyl compounds is thus avoided and direct injection of the sample into the HPLC system is allowed. The procedure offers a high specificity, since the derivatives of F and HMF are well separated from the other components of the mixture under examination. Recoveries of 95-99% were obtained from honey spiked at different levels with both analytes. The detection limit is of the order of 10/zg/kg of honey and reproducibility (mean of six determinations) is -+3% for F and +_2% tbr HMF.

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“…It can therefore be concluded that use of the method can save much time and money and it can be used in small laboratories with very high accuracy and a wide linear range. The method can be successfully used for routine analysis of aldehyde without interference (Papa and Turner 2 , 1972).…”

Section: High-performance Liquid Chromatographymentioning

confidence: 99%

Aldehyde, Ketone and Methane Emissions from Motor Vehicle Exhaust: A Critical Review

Kumar

2011

ACSJ

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Recent data indicate that, in many countries, mobile sources are responsible for the largest portion of emissions of aldehydes, ketones and certain other air toxic pollutants. These air toxic pollutants along with methane are either carcinogenic or pose significant human health threat. These pollutants also add to global warming in a substantial way. This paper gives an overview of their properties, basic chemistry and conditions of formation in internal combustion engines. Again worldwide many countries are promoting alternative fuels to tackle the crisis of traditional fuel. But the impact of this movement toward alternative fuels with respect to toxic emissions has not been well studied. Therefore, in this paper the analysis on the effects of engine operation and fuelling parameters is also reviewed with specific references in gasoline, diesel, natural gas, liquefied petroleum gas, ethanol blended petrol and Biodiesel fuelled engines. This is accompanied by the review of the studies of the performance of exhaust catalytic converters with respect to aldehydes, ketones and methane. Additionally, aldehydes, ketones and methane detection and measurement methods are summarized and analyzed from the view of their applicability to exhaust gas analysis.

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Chromatographic Determination of Carbonyl Compounds as Their 2,4-Dinitrophenylhydrazones II. High Pressure Liquid Chromatography

Cited by 75 publications

References 0 publications

Putative mechanism of the sugar formation on prebiotic Earth initiated by UV-radiation

Putative mechanism of the sugar formation on prebiotic Earth initiated by UV-radiation

High-performance liquid chromatographic determination of 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde in honey

Aldehyde, Ketone and Methane Emissions from Motor Vehicle Exhaust: A Critical Review

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