Chromatographic characterization of ion exchangers for high-performance liquid chromatography of proteins

Bentrop, D.; Engelhardt, H.

doi:10.1016/s0021-9673(01)96233-8

Cited by 16 publications

(2 citation statements)

References 15 publications

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“…28 The ion-capacity quantified by frontal analysis was 0.81 meq/g and by elemental analysis was 0.87 meq/g. These values are consistent showing a deviation of less than 10%.…”

Section: Strong Anion-exchange Derivatization and Ion-exchange Capacitymentioning

confidence: 98%

Temperature-independent Detection of Heteroduplex and Homoduplex Fragments Applying Poly(glycidylmethacryate-co-divinylbenzene-based) Monoliths Modified to Strong Anion-exchanger

Lubbad

2015

ACSi

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Monoliths of poly (glycidylmethacrylate-co-divinylbenzene) were prepared in the confines of presilanized borosilicate glass columns (100 × 3 mm I.D.). These monoliths were surface modified into strong anion-exchangers with hydrochloric acid (10%) and triethylamine, successively. The strong anion-exchanger established good separation of 5-phosphorylated oligodeoxythymidylic acids fragments [d(pT) [12][13][14][15][16][17][18] ]. Moreover, heteroduplex and homoduplex fragments of a low-range mutation standard [of STS marker from the Y-chromosome (209 bp)] were separated at ambient and elevated temperatures using sodium phosphate buffer and a gradient former of sodium chloride, in anion-exchange high-performance liquid chromatography (AE-HPLC). This is a step forward for mutation detection as temperature-independent method, which is not the case in denatured ion-paired reversed-phase chromatography (D-IP-RP-HPLC), where mutation detection is temperature critical and might be bypassed if temperature changes slightly. Finally, reproducibility check from run-to-run and monolith-to-monolith showed a relative standard deviation (RSD) of less than 2%.

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“…28 The ion-capacity quantified by frontal analysis was 0.81 meq/g and by elemental analysis was 0.87 meq/g. These values are consistent showing a deviation of less than 10%.…”

Section: Strong Anion-exchange Derivatization and Ion-exchange Capacitymentioning

confidence: 98%

Temperature-independent Detection of Heteroduplex and Homoduplex Fragments Applying Poly(glycidylmethacryate-co-divinylbenzene-based) Monoliths Modified to Strong Anion-exchanger

Lubbad

2015

ACSi

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“…18 Particle size of silica particles is usually determined by electron microscopy, light scattering or the Coulter-Counter method, 19,20 surface area by nitrogen adsorption experiments according to the Brunnauer Emmet Teller (BET) theory and 19,20 pore size and volume by size-exclusion chromatography (SEC). [21][22][23][24][25][26] These methods are time-consuming, expensive and require a lot of experience.…”

Section: Introductionmentioning

confidence: 99%

Near Infrared Spectroscopy, Cluster and Multivariate Analysis—Characterisation of Silica Materials for Liquid Chromatography

Huck

Ohmacht

Szabó

et al. 2006

Journal of Near Infrared Spectroscopy

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Near infrared (NIR) refl ectance spectroscopy is a well established method within the analytical chemistry and a wide range of applications, for example in the fi eld of food and pharmaceutical products, is covered. In the era of material science and nano technology, we brought together, in this feasibility study, silica chemistry with NIR. NIR, in the fi bre-optics mode, is used for morphological studies. It enables differentiation between silica materials in a three-dimensional factor-plot depending on their functionalisation and physical properties such as particle diameter and pore diameter with Q-values of 0.95 and 0.99. The well established, common reference methods for the determination of surface area and porosity are the so called Brunnauer Emmett Teller method and size-exclusion chromatography. Both methods are time-consuming and require a lot of experience. The recently elaborated NIR method offers a physicochemical quantitative description at the nano-scale level of particle size, specifi c surface area, pore diameter, pore porosity, pore volume and total porosity with high linearity of R 2 > 0.97 for calibration and r 2 > 0.98 for validation and a bias of < 2.48 × 10-14. Compared to the reference method, which enables the determination of the individual parameters with a relative standard deviation between 6 and 28%, the NIR method shows RSD% between only 0.010 and 13.7%, which means a fundamental improvevement in precision. The measurement takes only a few seconds so high sample throughput is guaranteed. The suitability of the newly established method in material science is demonstrated, as an example, on the silica stationary phases for liquid chromatography.

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Steric mass‐action ion exchange: Displacement profiles and induced salt gradients

1992

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Chromatographic characterization of ion exchangers for high-performance liquid chromatography of proteins

Cited by 16 publications

References 15 publications

Temperature-independent Detection of Heteroduplex and Homoduplex Fragments Applying Poly(glycidylmethacryate-co-divinylbenzene-based) Monoliths Modified to Strong Anion-exchanger

Temperature-independent Detection of Heteroduplex and Homoduplex Fragments Applying Poly(glycidylmethacryate-co-divinylbenzene-based) Monoliths Modified to Strong Anion-exchanger

Near Infrared Spectroscopy, Cluster and Multivariate Analysis—Characterisation of Silica Materials for Liquid Chromatography

Steric mass‐action ion exchange: Displacement profiles and induced salt gradients

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