Chromatographic characterisation of an estrogen-binding affinity column containing tetrapeptides selected by a combinatorial-binding approach

Tozzi, Cinzia; Anfossi, Laura; Giraudi, Gianfranco; Giovannoli, Cristina; Baggiani, Claudio; Vanni, Adriano

doi:10.1016/s0021-9673(02)00745-8

Cited by 26 publications

(23 citation statements)

References 7 publications

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“…These beads were also suitable for the synthesis work because of their large size (16-50 mesh) and their easiness to handle and separate. Also, this solid phase gave good results in liquid chromatography [10] and so these characteristics made the beads a suitable solid phase for packing SPE columns.…”

Section: Resultsmentioning

confidence: 95%

“…All the binding constants increased; moreover, we have to underline that the selectivities showed by the tetrapeptide agreed with the ones reported for a commercial antibody (third and fourth columns), although aflatoxin G 1 showed a binding constant similar to the one obtained for the dipeptide and therefore gave a lower selectivity. Furthermore, we could be quite optimistic about the binding properties of the tetrapeptide towards aflatoxins B 1 and B 2 as they showed a binding constant higher than 10 4 M -1 that could be sufficient for retaining the analytes in solution [10]; whereas we had to doubt the capacity of the tetrapeptide to bind the other two aflatoxins that showed slightly lower binding constants, above all for aflatoxin G 1 .…”

Section: Resultsmentioning

confidence: 99%

“…This solid phase with the spacer arm immobilised was used as a starting solid phase to prepare the amino acid libraries [10].…”

Section: Solid-phase Synthesesmentioning

confidence: 99%

“…Another approach for obtaining synthetic recognition systems could be combinatorial chemistry [10], which allows us to prepare great libraries of well-characterised compounds with different binding properties.…”

Section: Introductionmentioning

confidence: 99%

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A combinatorial approach to obtain affinity media with binding properties towards the aflatoxins

et al. 2003

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In our work we performed a combinatorial solid-phase synthesis in aqueous medium to prepare peptide libraries from which to select an amino acid sequence with binding properties towards aflatoxins. We used polystyrene beads, functionalised with carboxylic groups as solid support and eight amino acids as monomers. During the first step 64 different sequences of two amino acids were prepared by exploiting the principles of combinatorial chemistry; then the binding properties of all sequences towards aflatoxin B(1 )were checked. We determined binding constants towards aflatoxin B(1) and towards aflatoxins B(2), G(1) and G(2). Results were promising, so we prepared a new library by using the selected dipeptide as the starting solid phase. After selecting the best tetrapeptide sequences, we determined binding constants towards the quoted aflatoxins. We obtained binding constants ( K>10(4) M(-1)) similar to those shown by human serum albumin for similar compounds. Preliminary studies on an extraction column were promising for the development of an SPE system and for its application in food matrices.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

“…This solid phase with the spacer arm immobilised was used as a starting solid phase to prepare the amino acid libraries [10].…”

Section: Solid-phase Synthesesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A combinatorial approach to obtain affinity media with binding properties towards the aflatoxins

et al. 2003

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“…Although the analytical techniques are much more specific and sensitive, they are not affordable for most laboratories because of their specialty requirement, sophisticated sample preparation (from few hours to overnight hydrolysis), and the high cost of equipment. Other procedures use immunoaffinity [2, 29 -32], molecularly imprint [33], and tetrapeptide solid extraction phase columns [34]. However, their scope of application is limited to several compounds, and does not cover all hormones and hormonelike substances.…”

Section: Introductionmentioning

confidence: 99%

Analytical procedure to simultaneously measure trace amounts of trenbolone acetate and β‐trenbolone residues in porcine muscle using HPLC‐UVD and MS

Liu

El‐Aty

Choi

et al. 2008

J of Separation Science

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The current study was undertaken to validate the performance for the determination of both TBA and beta-trenbolone (beta-TB) residues in porcine muscle at concentrations required to monitor compliance with the maximum residue limit (MRL). The method involves a one phase liquid-liquid extraction, cleanup with low-temperature fat precipitation, separation of the respective compounds by HPLC on a Capcell pak C(18) column, use of a methanol-water isocratic system as an eluent, and measurement by UV absorbance detection at 340 nm. Both compounds were confirmed using LC-MS/MS with electrospray interface (ESI) and a triple quadrupole (QqQ) analyzer. The method was found to be precise and accurate, with a linearity range of 1-10 microg/kg (r(2) >0.973). The intra- and interday precision showed good reproducibility with RSDs < or =13.25%. The LODs were 0.12 and 0.22 microg/kg, and the LOQs were 0.37 and 0.66 microg/kg, for TBA and beta-TB, respectively. The applicability of the method was demonstrated by analyzing real samples collected from major cities in the Republic of Korea. No residues of the selected compounds were detected in any of the samples. The advantages of our method are that it is: selective, sensitive, requires a short time for analysis (13 min), and performs simple sample extraction and clean-up procedure with low-temperature fat precipitation as compared to the previously published methods.

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Towards a New SPE Material for EDCs: Fully Automated Synthesis of a Library of Tripodal Receptors Followed by Fast Screening by Affinity LC

et al. 2009

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A series of human estrogen receptor (hER) mimics were synthesised. On the basis of the knowledge on the structure of the hormone binding domain of the hER, three different peptide chains were constructed onto a tripodal scaffold. By using a fully automated solid-phase synthesis protocol, 120 members with known identity were synthesised in only a week. Affinity towards estrogenic compounds was checked by affinity LC. For this purpose, ethinylestradiol was "clicked" onto a modified-silica phase, and the obtained material was packed into an HPLC column. The results stemming from the affinity LC experiments were confirmed by clicking one library member to silica and by using this solid phase to extract different endocrine-disrupting chemicals (EDCs) from aqueous media

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Chromatographic characterisation of an estrogen-binding affinity column containing tetrapeptides selected by a combinatorial-binding approach

Cited by 26 publications

References 7 publications

A combinatorial approach to obtain affinity media with binding properties towards the aflatoxins

A combinatorial approach to obtain affinity media with binding properties towards the aflatoxins

Analytical procedure to simultaneously measure trace amounts of trenbolone acetate and β‐trenbolone residues in porcine muscle using HPLC‐UVD and MS

Towards a New SPE Material for EDCs: Fully Automated Synthesis of a Library of Tripodal Receptors Followed by Fast Screening by Affinity LC

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