Chromatographic Behavior of Uric Acid and Methyl Uric Acids on a Diol Column in HILIC

Quiming, Noel S.; Denola, Nerissa L.; Saito, Yoshihiro; Catabay, A. P.; Jinno, Kiyokatsu

doi:10.1365/s10337-008-0559-9

Cited by 39 publications

(20 citation statements)

References 42 publications

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“…A significant effect of the salt concentration in the eluent on retention properties of neutral-bonded HILIC phases was reported earlier [9,18,30] and also found in present study, both under AQ-and NA-HILIC conditions. Higher amounts of salt in AQ-HILIC mobile phases exerted mostly a pronounced positive effect on retention while in the NA-HILIC mode some analytes departed from this trend and showed even a remarkable decrease in retention (Fig.…”

Section: Effects Of Salt Concentrationsupporting

confidence: 90%

Hydrophilic interaction chromatography in nonaqueous elution mode for separation of hydrophilic analytes on silica‐based packings with noncharged polar bondings*

Bicker¹,

Lämmerhofer

et al. 2008

J of Separation Science

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Chromatographic effects of dedicated stationary and mobile phase variations in hydrophilic interaction chromatography (HILIC) were investigated using a set of nucleobases, nucleosides and deoxynucleosides as polar test solutes. Retention and selectivity profiles were comparatively mapped on four in-house developed silica materials modified with short alkyl chains (C4, C5) which carry hydroxyl functionalities (including diol motifs) as well as embedded sulphide or sulphoxide groups. These data were complemented by results obtained with two commercially available diol-type phases and a bare silica column. Besides elucidation of packing-related aspects this work concentrated specifically on extending aqueous HILIC (AQ-HILIC) to nonaqueous polar-organic elution conditions herein termed NA-HILIC. The exchange of the polar modifier water by various alcohols in ACN-rich mobile phases containing 5 mM ammonium acetate decreased the eluotropic strength of the resulting eluents. The gain in retention largely followed the order ethanol (EtOH)>methanol (MeOH)>1,2-ethanediol (Et(OH)2) and was accompanied by distinct effects on chromatographic selectivity. For example, on the most polar home-made packing the purine nucleoside selectivity guanosine/adenosine increased from 2.25 in the AQ-HILIC (kguanosine=8.3) to 7.33 (kguanosine=59) in the NA-HILIC mode when EtOH was employed as NA modifier while this value was 5.84 and 2.93 with MeOH and Et(OH)2, respectively (eluent: 5 mM ammonium acetate in ACN/modifier 90:10 v/v). Besides the type of protic modifier its percentage as well the retention and selectivity effects upon varying the ammonium acetate concentration and column temperature, respectively, were also investigated. Notable inter-column differences were found for all of these elution parameters. A mixed-mode retention model composed of partitioning and adsorption is proposed for both AQ- and NA-HILIC retention processes. The potential of (i) the implementation of novel polar bondings (such as ones containing sulphoxide functionalities) and (ii) the comprehensive exploitation of elution variables (type of protic modifiers, salt, etc.) for providing new selectivity increments to the separation of polar analytes in HILIC is emphasised.

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Section: Effects Of Salt Concentrationsupporting

confidence: 90%

Hydrophilic interaction chromatography in nonaqueous elution mode for separation of hydrophilic analytes on silica‐based packings with noncharged polar bondings*

Bicker¹,

Lämmerhofer

et al. 2008

J of Separation Science

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“…As displayed in the chromatograms, the higher acetate concentrations yielded stronger retention of benzoic acid on titania monolith. This phenomenon, increased retention with the increase of salt concentration, was in accordance with the previous results reported on a variety of HILIC columns [18][19][20][21][22]. The possible causes for this result are that, firstly, higher buffer concentration may drive the solvated salt to the water-enriched layer so that the water layer volume increases and therefore the retention time increases.…”

Section: Buffer Concentrationsupporting

confidence: 91%

Preparation of Titania Monolith Column and Application in Determination of Benzoic Acid by HILIC

Wang

Jiang

et al. 2012

Chromatographia

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The titania monolith column has been synthesized through a template-free sol-gel route, and a simple and reliable method for the determination of benzoic acid by hydrophilic interaction liquid chromatography using the prepared titania monolith has been developed. The influences of acetonitrile, acetate buffer and buffer pH on the retention of benzoic acid were investigated. Benzoic acid in carbonated drinks and fruit beverages samples were determined within 5 min and quantitative analysis was carried out by external standard method with a correlation coefficient (R 2 ) of 0.9984. The relative standard deviation was 0.91 % and the recovery ranged from 92.5 to 101.3 %. The proposed method is suitable for the analysis of benzoic acid in beverage samples.

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“…Development of novel fibrous extraction/separation media having different chemical structures and then different functionalities on the surface could be realized in the near future based on the concept of molecular shape recognition [3][4][5][6][7][8] and the systematic retention mechanism analysis with a help of various modern computational data calculations in LC [106][107][108][109][110][111][112][113][114][115][116][117][118]. Miniaturization of packed columns in GC [119][120][121][122][123] is also possible for high speed analysis with a unique selectivity but without a loss of sample loading capacity.…”

Section: Future Prospectivementioning

confidence: 99%

Miniaturization for the Development of High Performance Separation Systems

Saito

Ueta

2017

Chromatography

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Miniaturization is a key technological approach to realize high performance separation systems that have several important features required in modern analytical methods. A smaller scale of the stationary phase synthesis enables a systematic analysis of the retention behavior and also a more efficient development of novel stationary phases in liquid chromatography. Development of microscale sample preparation technique has been regarded as a valuable supporting approach to the development of microscale separation methods. Effective on-line coupling of the microscale sample preparation and separation allows an efficient and rapid analysis without a loss of sensitivity for most of the analytical separations. In this review, development of the miniaturized separation techniques and the applications have been described from the view point of the authors' research group, including the development of novel stationary phase, microscale sample preparation techniques for liquid and gas sample matrices.

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Chromatographic Behavior of Uric Acid and Methyl Uric Acids on a Diol Column in HILIC

Cited by 39 publications

References 42 publications

Hydrophilic interaction chromatography in nonaqueous elution mode for separation of hydrophilic analytes on silica‐based packings with noncharged polar bondings*

Hydrophilic interaction chromatography in nonaqueous elution mode for separation of hydrophilic analytes on silica‐based packings with noncharged polar bondings*

Preparation of Titania Monolith Column and Application in Determination of Benzoic Acid by HILIC

Miniaturization for the Development of High Performance Separation Systems

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