Abstract:In the present study, the RP-HPLC based separation and quantification of four common vitamins: B 1 , B 2 , B 6 , and folic acid in multivitamin pharmaceutical dosages was performed. Simultaneous determination of these vitamins was performed usin Promosil HPLC column C18 at 35 º C with methanol-0.05 M sodium acid phosphate (80 + 20, v/v) as mobile phase at pH 7.0 using UV-Visible detection system at wavelength 254 nm. Sample selection was made on the basis of products that declare the presence and mount of B gr… Show more
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