Chromatographic analysis of carbamazepine binding to human serum albumin

Studies by biointeraction chromatography of binding by phenytoin metabolites to human serum albumin

“…As demonstrated previously [7,19], this situation is described by Equation (5) even if I (but not A) has multiple binding regions on the ligand. In this case, the value of K IL that is obtained from a plot of 1/k versus [I] gives the association equilibrium constant for I at its site of competition of A.…”

“…The binding of m-HPPH and p-HPPH to HSA was studied by using the chromatographic method of zonal elution [16], [18], [19], [20], [21] and [22]. In this technique, a competing agent (I) is placed in the mobile phase at a known concentration and continuously applied to an affi nity column containing an immobilized ligand (L), such as HSA.…”

“…If A is known to bind to a given region on the ligand, this allows the competition of I at that site to also be examined and quantitated. By using different probe compounds as the injected analyte, each with a different binding region on the ligand, the interactions of I at other sites on the same ligand can be examined in the same manner [4,11,12,16,18,19].…”

“…The location of these binding regions will be identifi ed through studies using warfarin, l-tryptophan, digitoxin, and cis-clomiphene as site-selective probes [11,[16][17][18][19]. The competition of these phenytoin metabolites with phenytoin will also be considered.…”

Fases estacionárias quirais para cromatografia líquida de alta eficiência

“…Os resultados obtidos neste estudo confirmaram a existência de interação alostérica entre os sítios ativos dos benzodiazepínicos e da warfarina. 21 A literatura também descreve estudos das interações carbamazepina-proteína, 22 ibuprofeno/acetato de S-lorazepam, Soxazepam hemisuccinato/R-oxazepam hemisuccinato e L-triptofano/ fenitoína 23 em colunas de HSA e estudos das interações de diversos compostos ácidos, básicos e neutros em coluna de a 1 -glicoproteína ácida. 24 …”