Chromatofocusing profile of purified human α-fetoprotein and albumin differs from those of crude samples: Effect of protein concentration on the elution of the sample

Mammalian alpha-fetoproteln (AFP) Is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. Molecular variants of AFP have long been reported In the biomedical literature. Early studies Identified Isoelectrlc pH isoforms and lectin-binding variants of AFP, which differed In their physicochemical properties, but not In amino acid composition. Genetic variants of AFP, differing in mRNA kllobase length, were later extensively described in rodent models during fetal/perinatal stages, carcinogenesis, and organ regeneration. With the advent of monoclonal antibodies in the early 1980s, multiple antigenic epitopes on native AFP were detected and categorized, culminating In the Identification of six to seven major epltopes. During this period, various AFP-blndlng proteins and receptors were reported to inhibit certain AFP Immunoreactlons. Concomlttantly, human and rodent AFP were cloned and the amino acid sequences of the translated proteins were divulged. Once the amino acid composition of the AFP molecule was known, enzymatic fragments could be identified and synthetic peptide segments synthesized. Following discovery of the molten globule form In 1981,the existence of transitory, intermediate forms of AFP were acknowledged and their physiological significance was realized. In the present review, the various Isoforms and variants of AFP are discussed In light of their potential biological relevance.

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Chromatofocusing profile of purified human α-fetoprotein and albumin differs from those of crude samples: Effect of protein concentration on the elution of the sample

Cited by 4 publications

References 10 publications

Purification of alpha-fetoprotein from human cord serum with demonstration of its antiestrogenic activity

Purification of alpha-fetoprotein from human cord serum with demonstration of its antiestrogenic activity

Biochemical characterization of bovine alpha-fetoprotein and comparison with human alpha-fetoprotein

Alpha-fetoprotein Structure and Function: Relevance to Isoforms, Epitopes, and Conformational Variants

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