Chromatin structure and nucleic acid synthesis in human lymphocyte activation by phytohemagglutinin

Pompidou, A; Rousset, S.; Mace, B.; Michel, P.; Esnous, D.; Renard, Nathalie

doi:10.1016/0014-4827(84)90716-x

Cited by 27 publications

(13 citation statements)

References 26 publications

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“…Morphological analysis shows that significant chromatin decondensation and increased nuclear and nucleolar volume occur in cycling PWM-PBLs. This is in good agreement with previous studies (Pompidou et al, 1984). We also document, however, that a significant increase in both the size and number of interchromatin granule clusters occurs in cycling PWM-PBLs.…”

Section: Discussionsupporting

confidence: 93%

Modulation of the nuclear antigen p105 as a function of cell‐cycle progression

Clevenger

Epstein

Bauer

1987

Journal Cellular Physiology

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The characterization of the proliferation-associated nuclear antigen designated p105 in quiescent and proliferating lymphocytes is described. Through the use of novel flow cytometric and cell-sorting strategies the intracellular content of p105 was assessed in situ on a per cell basis. These analyses demonstrated the presence of multiple cellular subpopulations within the cell cycle differing significantly in p105 content. The data revealed that the flow cytometric quantitation of p105 levels may effectively discriminate cycling from noncycling cells. Immunogold electron microscopy revealed that the modulation of this interchromatin-associated antigen was correlated with a significant degree of nuclear restructuring. In conjunction with cell sorting, immunogold electron microscopy and immunoblot controls demonstrated that the cell-cycle-related modulation in p105 cannot be accounted for by increased cellular mass or antigen sequestration. The significance of these controls and of the potential role of p105 in cellular proliferation is discussed.

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Section: Discussionsupporting

confidence: 93%

Modulation of the nuclear antigen p105 as a function of cell‐cycle progression

Clevenger

Epstein

Bauer

1987

Journal Cellular Physiology

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“…Contrasting differentiation, lymphocyte activation is much more rapid and dynamic and involves significant genome restructuring (Drings and Sonnemann 1974). Compacted peripheral chromatin dissipates upon activation concomitant with large-scale gene activation (Pompidou et al 1984). Our previous finding of changes in nuclear membrane protein composition during lymphocyte activation (Korfali et al 2010) led us to investigate whether there are also specifically regulated gene exchanges at the nuclear envelope.…”

Section: [Supplemental Materials Is Available For This Article]mentioning

confidence: 99%

“…The extensive electron-dense peripheral heterochromatin of resting T cells dissipates during activation concomitantly with the induction of immunogenic genes (Hirschhorn et al 1971;Pompidou et al 1984;Manteifel et al 1992;Rawlings et al 2011). However, it is unclear whether this reorganization is wholescale or whether there is also more specific reorganization for immune activation.…”

Section: Mapping Gene Expression and Repositioning Changes During T-cmentioning

confidence: 99%

Constrained release of lamina-associated enhancers and genes from the nuclear envelope during T-cell activation facilitates their association in chromosome compartments

et al. 2017

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The 3D organization of the genome changes concomitantly with expression changes during hematopoiesis and immune activation. Studies have focused either on lamina-associated domains (LADs) or on topologically associated domains (TADs), defined by preferential local chromatin interactions, and chromosome compartments, defined as higher-order interactions between TADs sharing functionally similar states. However, few studies have investigated how these affect one another. To address this, we mapped LADs using Lamin B1-DamID during Jurkat T-cell activation, finding significant genome reorganization at the nuclear periphery dominated by release of loci frequently important for T-cell function. To assess how these changes at the nuclear periphery influence wider genome organization, our DamID data sets were contrasted with TADs and compartments. Features of specific repositioning events were then tested by fluorescence in situ hybridization during T-cell activation. First, considerable overlap between TADs and LADs was observed with the TAD repositioning as a unit. Second, A1 and A2 subcompartments are segregated in 3D space through differences in proximity to LADs along chromosomes. Third, genes and a putative enhancer in LADs that were released from the periphery during T-cell activation became preferentially associated with A2 subcompartments and were constrained to the relative proximity of the lamina. Thus, lamina associations influence internal nuclear organization, and changes in LADs during T-cell activation may provide an important additional mode of gene regulation.

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“…Because alterations in cellular morphology are frequently associated with changes in cellular function (33,34), we next wanted to explore whether CD44 stimulation resulted in the functional activation of the hybridomas. As with the morphology experiments, we used plate-bound CD44-specific (IM7) and isotype control Abs to assess cytokine release from the hybridomas as a primary measure of their activation state.…”

Section: Ab-specific Cross-linking Of Cd44 Activates Nkt But Not Cd4mentioning

confidence: 99%

CD44 Differentially Activates Mouse NK T Cells and Conventional T Cells

Larkin

Renukaradhya

Sriram

et al. 2006

The Journal of Immunology

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NK T (NKT) cells are an important component of the innate immune system and recognize the MHC class I-like CD1d molecule. NKT cells possess significant immunoregulatory activity due to their rapid secretion of large quantities of pro- and anti-inflammatory cytokines following CD1d-dependent stimulation. Because the innate immune system is programmed to respond to a multitude of diverse stimuli and must be able to quickly differentiate between pathogenic and endogenous signals, we hypothesized that, apart from stimulation via the TCR (e.g., CD1d-dependent activation), there must be multiple activation pathways that can be triggered through other cell surface receptors on NKT cells. Therefore, we analyzed the ability of CD44, a structurally diverse cell surface receptor expressed on most cells, to stimulate murine NKT cells, compared with conventional T cells. Stimulation of CD44 through Ab cross-linking or binding to its natural ligands hyaluronan and osteopontin induced NKT cells to secrete cytokines, up-regulate activation markers, undergo morphological changes, and resist activation-induced cell death, whereas conventional T cells only exhibited changes in morphology and protection from activation-induced cell death. This CD44-specific stimulation of NKT cells correlated with their ability to bind hyaluronan. Thus, fundamental differences in CD44 function between these lymphocyte subsets suggest an important biological role for CD44 in the innate immune response.

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Chromatin structure and nucleic acid synthesis in human lymphocyte activation by phytohemagglutinin

Cited by 27 publications

References 26 publications

Modulation of the nuclear antigen p105 as a function of cell‐cycle progression

Modulation of the nuclear antigen p105 as a function of cell‐cycle progression

Constrained release of lamina-associated enhancers and genes from the nuclear envelope during T-cell activation facilitates their association in chromosome compartments

CD44 Differentially Activates Mouse NK T Cells and Conventional T Cells

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